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Effect of Gamma Radiation on the Pupal Radiosensitivity and Male Sterility of the Peach Fruit Fly, Bactrocera Zonata (saunders) (diptera: Tephritidae)

M.A. Hossain
Principal Scientific Officer
Insect Biotechnology Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Ganakbari, Savar, Dhaka-1349, Bangladesh

S.A. Khan
Chief Scientific Officer
Insect Biotechnology Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Ganakbari, Savar, Dhaka-1349, Bangladesh

A.J. Howlader
Professor
Department of Zoology, Jahangirnagar University, Dhaka-1342, Bangladesh.

Abstract/ Executive Summary:

An experiment was conducted to know the effect of gamma radiation on the pupal radiosensitivity and male sterility of the peach fruit fly. Radiosensitivity of pupae was found to be decreased as the age increased. No adults were emerged from 1, 2, 3, 4, 5 and 6 day old pupae treated with 40, 150, 200, 300, 350 and 400 Gy doses accordingly. The LD50 values for 1, 2, 3, 4, 5 and 6 day old pupae were 23.98, 31.18, 38.73, 96.93, 112.07 and 123.55 Gy respectively. It revealed that late aged pupae are comparatively radioresistant than that of early aged pupae. Gamma ray significantly (p<0.01) increased the pupal duration and decreased the longevity of the peach fruit fly. The sterilizing dose of 60 Gy was recorded for 6 days old pupae of B. zonata.

Keywords: Gamma radiation, Sensitivity, Sterility, Fruit fly

Location: Insect Biotechnology Division, Institute of Food and Radiation Biology (IFRB), Atomic Energy Research Establishment (AERE), Savar, Dhaka

Start Date:

End Date:

Program Area: Pest Management

Commodity: Insects

Objectives:

To study the effect of Gamma radiation on the pupal radio sensitivity and male sterility of the peach fruit fly, Bactrocera zonata (Saunders).

Methodology:

Laboratory cultures of B. zonata were maintained in the fruit fly laboratory, Insect Biotechnology Division, Institute of Food and Radiation Biology (IFRB), Atomic Energy Research Establishment (AERE), Savar, Dhaka. Larvae were maintained on wheat bran and sweet potato based artificial diet. Adult peach flies were stocked in a wooden frame cage (60×50×45 cm) covered with nylon net. The culture was usually supplied with an artificial diet (yeast extract: sugar: caesin/1:2:1) and water soaked cotton. In general, 1500-2000 adult fruit flies were maintained in a stock cage. Temperature and relative humidity of the rearing room were maintained at 28±2^oC and 70-80% respectively. The larvae of B. zonata in the artificial larval diets were kept in plastic bowls with saw dusts at the bottom for pupation. The saw dusts were sieved after pupation for the collection of dust free pupae. Collected 100 pupae each of 1, 2, 3, 4, 5 and 6 days old were transferred into Perti-dishes and irradiated in a Co⁶⁰ gamma irradiator of Institute of Food and radiation Biology, Atomic Energy Research Establishment, Savar, Dhaka. For pupal radiosensitivity studies, radiation doses ranging from 10 to 400 Gy were applied and for male sterility dose determination radiation doses ranging from 10 to 70 Gy were applied with a dose rate of 10 Gy/minute. Each dose group had 3 replications and was repeated 5 times. One batch of control was also maintained with equal number of replications. Fifty male flies were collected from each of 10, 20, 30, 40, 50, 60 and 70 Gy treated 6 days old pupae and fifty females were also isolated from the control batch and allowed to mate with individual dose treated males in laboratory rearing cages. This experiment had 5 replications. After 2 days of mating, eggs were collected from a piece of banana placed inside as oviposition medium. Data on pupal duration, deformity, adult emergence and longevity were recorded for analysis. Corrected mortality was calculated using Abbott’s formula (Abbott 1925). Probit analysis was done according to Finney (1947). ANOVA was done using Microsoft Excel Programme.

Source of Information: Int. J. Expt. Agric. 7 (3):14-18 (November 2017)

Article Link (Online Journal): http://ggfjournals.com/e-journals archive

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Data generated from this experiment on sterility dose, pupal duration and longevity of irradiated males of B. zonata clearly revealed that 60 Gy could be used as a sterilizing dose for possible field application in SIT and adult emergence data indicated that 400 Gy dose completely stopped the emergence of peach fruit fly. Further research on male competitiveness of sterile males and male ratio optimization will be required to generate a more suitable data for field application of SIT.

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