Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Rhizobiumsp. BARIrgm901 Inoculation Response to Soybean Genotypes in Northern Region of Bangladesh

F. ALAM
Senior Scientific Officer
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

M.W. RAHMAN
Scientific Officer
Breeder Seed Production Centre (BSPC), Bangladesh Agricultural Research Institute, Debigonj, Bangladesh

M.M. HOSSAIN
Senior Scientific Officer
Regional Agricultural Research Station, Burirhat, Rangpur, Bangladesh

M.B. BANU
Scientific Officer
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

S.S. ALAM
Scientific Officer
Plant Breeding Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

M.A.H. BHUIYAN
Principal Scientific Officer
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

Abstract/ Executive Summary:

A field experiment was conducted by soybean (Glycine max L. Merill) genotypes with Rhizobium sp. BARIRGm901inoculation at the Breeder Seed Production Centre (BSPC), Bangladesh Agricultural Research Institute (BARI), Debigonj, Panchagarh, Bangladesh during 2009-2010 and 2011-2012 with the objectives to find out the response of Rhizobium sp. BARIRGm901 inoculation with different plant genotypes of soybean. Two soybean varieties viz. Shohag and BARI Soybean-6, and two advance lines MTD-10 and BGM-02026 were studied with and without Rhizobium sp. BARIRGm901inoculationin Factorial Randomized Complete Block Design (RCBD) with four replications. Peat based rhizobial inoculum (strain BARIRGm901) was used at the rate of 1.5 kg ha^-1 as seed inoculant. Rhizobium sp. BARIRGm901inoculation significantly increased nodulation, dry matter production, pods plant^-1 and seed yields of soybean in both years. Nodulation was higher in BARI Soybean-6 and this variety also performed better in respect of shoot weight, pods per plant, stover and seed yield. Among the varieties and advance lines, the highest seed yield 1.73 t ha^-1 in 2009-2010 and 1.44 t ha^-1 in 2011-2012 was recorded. Interaction effects revealed that inoculated BARI Soybean-6 gave the highest seed yield (1.84 t ha^-1 in 2009-2010 and inoculated BGM- 02026 (1.60 t ha^-1) in 2011-2012. Nodule number and nodule weight was the highest in inoculated BARI Soybean-6 in both years. Therefore, Rhizobium sp. BARIRGm901inoculation established a symbiotic relationship with soybean genotypes and thus increased the nodulation, growth, and yield of soybean grown in northern region of Bangladesh.

Keywords: Advanced line, Inoculum, Nodulation, Biofertilizers

Location: Breeder Seed Production Centre (BSPC), Bangladesh Agricultural Research Institute (BARI), Debigonj, Panchagarh (Old Himalayan Piedmont Plain), Bangladesh.

Start Date: 00-00-2009

End Date: 00-00-2012

Program Area: Variety and Species

Commodity: Soybean

Objectives:

1. To study the effects of inoculated Rhizobium sp. BARIRGm901strain on nodulation, dry matter production and yield of soybean (Glycine max L. Merill) varieties/advance lines, and

2. To determine the main and interaction effects of Rhizobium sp. BARIRGm901strain and soybean varieties under northern region soils of Bangladesh.

Methodology:

The experiment was conducted on 20 December 2009 and 13 December 2011 at the Breeder Seed Production Centre (BSPC), Bangladesh Agricultural Research Institute (BARI), Debigonj, Panchagarh (Old Himalayan Piedmont Plain, AEZ-1; latitude 26°11´N, and longitude 88°73 E), Bangladesh. The experiment was laid out in Factorial Randomized Complete Block Design (RCBD) with four replications. The treatments included four soybean genotypes, two soybean varieties (Shohag and BARI Soybean-6), and two advance lines (MTD-10 and BGM-02026) which were each studied with and without Rhizobium (Rhizobium sp. BARIRGm901) inoculation. The unit plots were 2 × 3 m in size and seeds were planted at 70 kg ha^-1. The seeds of newly released varieties and advanced lines were collected from Oilseed Research Center of BARI, Gazipur, Bangladesh. Rhizobiumsp. was grown in YEM liquid medium to a population density of 1.2 × 10⁸ (CFU ml^-1, estimated by serial dilution method) and injected into peat soil (100 g packet). The peat based rhizobial inoculum was used in field experiment at 1.5 kg ha^-1. Soybean seeds were mixed thoroughly with the peat based inoculum and small quantity of water before sowing. Fertilizers were used at the rate of 22/42/40/5 kg PKSZn ha^-1, respectively, as a basal dose. Triple super-phosphate, muriate of potash, gypsum and zinc sulphates were used as the sources of phosphorus, potassium, sulphur and zinc, respectively. Weeding, thinning and other intercultural operations were done when necessary. Nodules were collected by carefully uprooting five sample plants selected randomly from each unit plot at the 50% flowering stage. The crop was finally harvested on 15 April 2010 and 25 April 2012. Nodules were separated from the roots aseptically during harvesting and nodule properties were studied without damaging roots and biomass. Fresh weight of roots and above ground biomass was carefully measured. The samples of nodules, root, and above ground biomass were dried at 70ºC for 72 h for dry weight measurements. The dried plant samples were ground and stored for further chemical analysis. At maturity stage, five plants were sampled randomly from each plot, pods were counted for all the five plants and the average value was reported as number of pod per plant. The soybean pods, stover (leaves with stem) and seeds were separated from plants and measured the fresh weight. The pods, stover, and seeds were dried at 70ºC for 72 h for measurements of dry weight of yield parameters. Soil samples were collected, air dried, and ground (2 mm sieve) for chemical analysis. Soil pH was measured (soil: water, 1:5 w/v) by a combined glass calomel electrode (Jackson 1958). Organic carbon was determined by wet oxidation method (Walkley and Black). Total N was determined by modified kjeldahl method. Soil samples were digested with di-acid mixture (HNO₃:HCLO₄ 3:1 v/v). The extract containing Ca, K and Mg were determined by NH₄OAc extraction method. Copper, Fe, Mn and Zn were determined by DTPA extraction followed by AAS reading. Boron was determined by CaCl₂ extraction method. Phosphorus was determined by Bray and Kurtz method. Sulphur was determined by CaH₄(PO₄)₂.H₂O extraction followed by turbidimetric method with BaCl_2.Statistical analyses will be conducted using standard statistical procedures implement in Statistix 10. The data will be examined by analysis of variance (ANOVA) and differences between the treatments will be determined by Fisher’s protected least significant difference (LSD) will be calculated at the 0.05 probability level for treatment mean comparisons.

Source of Information: J. Soil Nature 9 (2):11-19 (November 2016)

Article Link (Online Journal): http://ggfjournals.com/e-journals archive

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The results of the present study have indicated that it is important to promote the appropriate use of biofertilizers through national fertilizer programs. Efforts should be made, wherever possible, to introduce inoculation technology to the farming community. More research based on standard methods needs to be undertaken to assess the contribution of nitrogen-fixing plants to the overall nitrogen budget. The use of rhizobial inoculants is at its infancy in Bangladesh, and so far it is not one of the research priorities of Agricultural Research Institutions in the country. Rhizobial inoculants are not locally available, and farmers are not aware about this new technology. Therefore, more efforts need to be done to popularize this cheap and eco-friendly technology among resource poor farming community of the nation. For an alternative use, Rhizobium sp. BARIRGm901 can be recommended for soybean inoculum production.

Knowledge Management: Journal