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Comparative Serological Responses and Protection Conferred by Vaccination with V4hr and Bcrdv in Chickens

M. A. Kafi
Animal Health Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh.

M. B. Rahman
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. M. Amin
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. R. Islam
Animal Health Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh.

M. M. Rahman
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. K. Rahman
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

The serological responses and protection of Sonali breed chickens with Australian Newcastle disease V4 heat resistant ( NDV4HR ) live freeze-dried vaccine ( Australian Webster Pvt. Ltd., Sydney ) was compared with that of locally produced conventional Lentogenic F-strain Baby Chick Ranikhet Disease vaccine ( BCRDV, DLS, Dhaka ) of Bangladesh. Thirty-day-old chicks were purchased from Mirpur Govt. Poultry Farm, Dhaka and maintained hygienically with commercial feed and water supply ad libitum during the experimental period from November 2002 to January 2003. These birds were divided into three groups ( A, B and C ), each consisting of 10 birds. Each birds of group A was vaccinated with NDV4HR and group B with BCRDV intraocularly. primary vaccination at 7 days and booster vaccination at 28 days of age, whereas the birds of group C kept as control. Sera samples of each of the birds of all the three groups were collected at 14 days of post-vaccination following each of the primary and booster vaccination at 23 and 44 days of the age of birds. Each of the serum samples of all the three groups of birds was titrated by using the haemagglutination inhibition ( HI ) test and results recorded that both the NDV4HR ( 32.49 t 23.94 ) and BCRDV ( 28.28 t 10.54 ) produced more or less similar serological responses at two weeks after booster vaccination. The results of the challenged experiment showed that the NDV4HR vaccine ( 80% ) apparently conferred higher protection to birds than the BCRDV vaccine ( 70% ). Therefore both the vaccines may be recommended to control ND in commercial chickens but NDV4HR vaccine could provide a practical method of control ND in rural scavenging chickens.

Keywords: Serological responses, F-strain (BCRDV), NDV4HR vaccine, HI antibody titre, Protection, Chickens

Location: The experimental house of the Department of Microbiology and Hygiene, BAU, Mymensingh, during the period from November 2002 to January 2003.

Start Date: 00-11-2002

End Date: 00-01-2003

Program Area: Animal Health and Management

Commodity: Chicken, Vaccine

Objectives:

To determine the Comparative Serological Responses and Protection Conferred by Vaccination with V4hr and BCRDV in Chickens.

Methodology:

ad libitum throughout the experiment. The Australian heat resistant V4 strain of ND virus (NDV4HR) vaccine (obtained from Dr. Jonathan Bell, Senior Advisor, SLDP-2 project ) and F strain ( Baby Chick Ranikhet Disease Vaccine, BCRDV) vaccine locally produced by the Director of Livestock Services ( DLS ) were used for this comparative trial experiment. Each bird of group A were inoculated with NDV4HR and each bird of group B was inoculated with BCRDV intraocularly @ 2 drops/bird two times at the age of 7 and 28 days, whereas the birds of group C kept as unvaccinated control. Blood samples were collected from the wing vein of each of the thirty birds of all the three groups at the age of 6 days, Le., one day before of primary vaccination and at the age of 23 days, i.e., two weeks after the primary vaccination and 44 days, i.e., two weeks after booster vaccination. Sera were separated and stored at —20° C until tested. Each of the collected serum samples was titrated for NDV antibody using the Haemagglutination Inhibition ( HI ) test by using reference Newcastle disease HA antigen ( Nisseiken Co. Ltd., Japan ) as per the method of Allan and Gough ( 1974 ). Each of 20 vaccinated birds of group A and B, and. of 10 unvaccinated control birds was challenged IM at the age of 45 days, i.e.,16 days of post-booster vaccination @ 0.25 ml of 103 EID50 NDV obtained from Department of Microbiology and Hygiene, BAU, Mymensingh. Challenged birds were observed for two weeks twice daily. Any bird showing the symptoms of ND was recorded, and necropsy examination was conducted in all the dead birds. Results were analyzed statistically by using a computerized statistical program ( SPSS, Version-7.5 ).

Source of Information: Bangl. J. Vet. Med. ( 2003 ). 1 ( 1 ) : 25 - 27

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The results of the present study indicate that both the locally produced conventional BCRDV and Australian NDV4HR vaccines stimulated similar and satisfactory humoral responses that are subjected to protect a virulent challenge. Therefore both vaccines could be recommended for field use but vaccination with NDV4HR vaccine could be an easy and effective method to immunize chickens.

Knowledge Management: Journal