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Tiba Induced Morphological and Root Anatomical Changes in Lentil

Saiful Islam
Plant Physiology Laboratory, Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Abstract/ Executive Summary:

A field experiment was conducted to evaluate the effect of 2,3,5-triiodobenzoic acid (TIBA) at 0, 10, 20, 30 and 40 ppm on the morphological and root anatomical changes of lentil (Lens culinaris Medik. cv. BARI Mosur-7). Foliarly applied TIBA decreased plant height significantly with the increasing concentrations of TIBA. Significantly maximum number of branches and leaves per plant were recorded due to 10 ppm TIBA treatment. Leaves were darker green in plants receiving 10 ppm TIBA due to high chl-a, -b and low carotenoid contents compared to control. Ten ppm TIBA increased root length significantly and root diameter non-significantly than the control plants. Root diameter in plants treated with 10 ppm TIBA increased due to the increase in the width of the cortex, decrease in vessel diameter and enhancement of the formation of more secondary xylem vessels.

Keywords: Mosur, TIBA, Foliar application, Growth, Anatomy

Location: Bangladesh Agricultural Research Institute), Joydebpur, Gazipur

Start Date: 00-00-2017

End Date: 00-00-2018

Program Area: Variety and Species

Commodity: Lentil

Objectives:

To study the effects of Triiodobenzoic acid (TIBA) on the morphological and root anatomical changes of lentil.

Methodology:

A field experiment was carried out at the research field of the Department of Botany, University of Dhaka during Rabi (Nobember- March) season of 2017-2018. Seeds of BARI Mosur-7 were collected from BARI (Bangladesh Agricultural Research Institute), Joydebpur, Gazipur. The experimental soil was analyzed and low levels of potassium and very low levels of nitrogen and phosphorus were recorded (FRG, 2012). Cowdung, urea, TSP and MoP were applied as basal amount during final land preparation. The experiment was laid out in randomized block design (RBD) with four replications. The unit plot size was 2 m×1.1 m. Seeds were sown on 29^th November, 2017 in rows having a gap of 40 cm. Plants in rows were maintained 15 cm apart by thinning seedlings at 15 days after sowing. Intercultural operations were done as and when necessary. Five foliar treatments viz., water spray (control), 10, 20, 30 and 40 ppm TIBA were tested against lentil var. BARI Mosur-7. The foliar spray was done at 45 days after sowing by using separate sprayers. Three weeks after treatment, control plants and TIBA treated plants were collected. Data on length of stem and root, number of branches and leaves per plant, stem and root diameter were recorded. The plants were preserved in FAA (formalin-acetic acid-alcohol) solution for anatomical study. Free hand cross sections of root were taken and stained in safranin. Root sections were taken 0.5 cm below the root collar. Photomicrograph of the stained specimens were taken using Trinocular Res. Microscope with digital camera system (Axiocam ERc 5s, Zeiss, Germany). Crude leaf chlorophyll extracts were made using 80% acetone. Spectrophotometer (UV-1800, Shimadzu) readings were recorded at 663, 645, 440.5 nm and the concentrations of chlorophyll-a and -b were determined by using specific absorption coefficients of MacKinney (1941). The amount of carotenoid was determined by the equation of Von Wettstein (1957). The data were compiled and subjected to statistical analysis and treatment means were compared for significance by using statistical program software ‘R’ at 5 % level of probability.

Source of Information: J. Bangladesh Acad. Sci., Vol. 43, No. 2, 107-112, 2019

Article Link (Online Journal): DOI: https://doi.org/10.3329/jbas.v43i2.45731

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The study confirms that the effect of TIBA on the induction of morphological and anatomical changes may be mediated by changing the hormonal balance of the plant. Of all the five treatments, application of TIBA at 10 ppm showed positive stimulation.

Knowledge Management: Journal