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Impact of Short and Long Term Heat Induced Treatment on Blow Fly Egg Tolerance (lucilia Cuprina, Diptera: Calliphoridae) for an Effective Control/suppress Program

Muhsina Yasmin
Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Gonakbari, Ashulia, Savar, Bangladesh Atomic Energy Commission, Agargoan, Dhaka-1207

Mahmudul Hossain
Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Gonakbari, Ashulia, Savar, Bangladesh Atomic Energy Commission, Agargoan, Dhaka-1207

Md. Shahinur Islam
Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Gonakbari, Ashulia, Savar, Bangladesh Atomic Energy Commission, Agargoan, Dhaka-1207

A.T.M. Fayezul Islam
Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Gonakbari, Ashulia, Savar, Bangladesh Atomic Energy Commission, Agargoan, Dhaka-1207

Abstract/ Executive Summary:

The farmers of fish drying areas of Bangladesh are facing a great tricky in controlling the pests of sun-drying fishes and most of these invasions occurred by blowfly (Lucilia cuprina). The present trial is to investigate the blowfly egg tolerance towards a heat-induced treatment with a view to developing an effective blowfly control/suppression program. In this experiment, both a short and long-term heat treatment were persuaded through a water bath system on 1.5-hour old blowfly eggs at 35⁰C and 37.5 ⁰C of temperature respectively. A control batch was also placed at room temperature (28± 2 ⁰C). In the case of 5 minutes heat induction, the egg hatching was significantly (p <0.01) higher than that of the 45 minutes of heat exposure. Only 3.6 ± 0.74 % of the egg was hatched at 37.5 ⁰C through a 4-hour long heat treatment. The observation disclosed that the longer the heat exposure the shorter the percentage of egg hatching. Again, it was revealed that 35 ⁰C of temperature is flawless for a higher number of eggs hatching by short-term heat exposure and 37.5 ⁰C is acknowledged as the best temperature regime for the highest egg mortality.

Keywords: Blow fly, Heat treatment, Short and long term, Egg hatching

Location: Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Gonakbari, Ashulia, Savar, Bangladesh Atomic Energy Commission, Agargoan, Dhaka-1207

Start Date:

End Date:

Program Area: Postharvest and Agro-processing

Commodity: Fish

Objectives:

To investigate the blowfly egg tolerance towards a heat-induced treatment with a view to developing an effective blowfly control/ suppress program.

Methodology:

Collection of L. cuprina egg- Egg clusters (1.5 hr old) of L. cuprina were collected in a petridish from the laboratory stock (reared on fish) maintained at a temperature of 25± 2⁰C and 65± 5 % of RH. There were five replications and a control batch also. Each cluster of eggs was immersed into six different small test tubes with normal tap water. The test tubes were vigorously shaken in order to break the cluster of eggs. Heat treatment- The eggs were exposed to hot water of the water bath ((BS- 06, JEIO TECH) in which the temperature was calibrated at 35⁰C and 37.5⁰C respectively, and was confirmed with a standard thermometer. The heat was tempted on the egg stage for a short period of 5, 15, 30, 45 minutes and a long period of 1, 2, 3, and 4 hours at 35⁰C and 37.5 ⁰C of temperature. Eggs for the controlled batch were placed in a test tube containing water of controlled temperature (28± 2⁰C) for respective time duration. Counting of Egg hatching- After the treatment, the eggs from the test tubes were placed in six different petridish that contained water-soaked cotton and black cloth. 100 eggs were positioned in a line with a brush on the black cloth and kept for hatching. After hatching the petridish were held under the microscope (Liedet, MZ- 720X) to observe the condition of the eggs and notice the number of hatches. The number of unhatched eggs was defined as the number of egg mortality. Finally, the percentage of male and female adult emergence will be checked in order to detect female embryonic lethality. Data analysis- All the data were analyzed via one-way ANOVA (analysis of variance) by Microsoft Excel (XLSTAT by Addinsoft) and detected as significant through P-value and F value estimation at p< 0.01 level.

Source of Information: International Journal of Biosciences - Vol. 16, No. 2, p. 137-142, 2020

Article Link (Online Journal): http://dx.doi.org/10.12692/ijb/16.2.137-142

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The present study and the above literature from the previous discussions indicate a contextual signal for the production of male flies by heat induction on eggs and also a proposition for the farmers for rescuing their sun-drying marine fish from blowfly infestation in the coastal area of Bangladesh.

Knowledge Management: Journal