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Assessment of Chlorophyll Loss Due to Infestation of Gall Mite in Bay Leaf

M. M. Hossain
Scientific Officer
Spices Research Centre (SRC), Bangladesh Agricultural Research Institute (BARI), Shibganj, Bogura, Bangladesh

K. M. Khalequzzaman
Principal Scientific Officer (Plant Pathology)
Spices Research Centre (SRC), Bangladesh Agricultural Research Institute (BARI), Shibganj, Bogura, Bangladesh

M. A. Sarkar
Senior Scientific Officer
Entomology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh

D. Sarker
Principal Scientific Officer
Entomology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh

Abstract/ Executive Summary:

The field experiment was conducted at Spices Research Centre, BARI, Shibganj, Bogura, Bangladesh during May, 2018 to analyze the percent loss of chlorophyll a and b induced by Aceria doctersi and to assess the damage potential of gall mite within the leaf galls of Cinnamomum tamala. The highest chlorophyll a (8.26 milligram/g) and chlorophyll b (2.31 milligram/g) content was recorded from control sample containing no gall tissue and the lowest chlorophyll a (2.20 milligram/g) and chlorophyll b (0.66 milligram/g) content was recorded from the sample containing ≥50 gall /1g leaf sample. Eriophyid mite galls were negatively correlated with chlorophyll a (r= -0.98245**) and chlorophyll b (r= -0.95525**) content of bay leaf.

Keywords: Assessment, Chlorophyll, Infestation, Gall mite, Bay leaf

Location: Spices Research Centre, BARI, Shibganj, Bogura, Bangladesh

Start Date: 01-05-2018

End Date: 31-12-2018

Program Area: Pest Management

Commodity: Mite, Bay Leaf

Objectives:

To analyze the percent loss of chlorophyll a and b induced by Aceria doctersi and to assess the damage potential of gall mite within the leaf galls of Cinnamomum tamala.

Methodology:

The study was conducted at Spices Research Centre, BARI, Shibganj, Bogura, Bangladesh (geographic coordinates 25.0167° N, 89.3167° E) during May, 2018 to analyze the percent loss of chlorophyll a and b induced by Aceria doctersi and to assess the damage potential of gall mite within the leaf galls of Cinnamomum tamala. BARI Tejpata-1 variety was used for the study. For this, two sets representing the experimental and control samples were selected. The experiment was carried out in May when the gall formation was found maximum in the field. For quantitative assessment of chlorophyll, 1 gram tissue each from the normal and galled leaves tissues bearing 10, 20, 30, 40, >50 galls, respectively were weighed out separately. Each sample cut into small pieces and then ground into a fine pulp in a clean mortar with the addition of 10 ml of 80% acetone (Nasareen and Ramani, 2014). The pulp prepared of each sample was then centrifuged individually at 5000 rpm for 5 minutes and the supernatant was transferred separately to volumetric flasks and made up to 100 ml with 80% acetone. The procedure was repeated until the residue became colorless. The absorbance of each solution was measured at 645 nm and 663 nm against the solvent blank in a UV-VIS spectrophotometer.

The amount of chlorophyll ‘a’/ gram tissue was calculated based on the following formula Ekanayaka and Adeleke (1996);

Milligram chlorophyll ‘a’/ gram tissue = 20.2 (A 645) (50/1000) (100/5) (1/2) and

Milligram chlorophyll‘b’/ gram tissue = 8.02 (A 663) (50/1000) (100/5) (1/2),

Where A= Absorbance at specific wavelength.

The experiment was repeated 5 times with the normal and control samples for the confirmation of results. Data on chlorophyll a (Milligram/g) and chlorophyll b (Milligram/g) were recorded. Percent loss of chlorophyll a and chlorophyll b content of bay leaf were calculated from chlorophyll a (Milligram/g) and chlorophyll b (Milligram/g) over control sample, respectively. The recorded data were analyzed and mean values were adjusted and separated by Duncan’s Multiple Range Test (DMRT) according to Gomez and Gomez (1984). Percent chlorophyll a and chlorophyll b reduction over control was calculated using following formula of Dutta et al. (2014).

Source of Information: Asian Journal of Applied Science and Engineering, 6(3), 123-130; (2017). ISSN 2305-915X(p); 2307-9584(e)

Article Link (Online Journal): URL: https://abc.us.org/ojs/index.php/ajase/article/view/150/315

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The highest chlorophyll a (8.26 milligram/g) and chlorophyll b (2.31 milligram/g) content was recorded from control sample containing no gall tissue and the lowest chlorophyll a (2.20 milligram/g) and chlorophyll b (0.66 milligram/g) content was recorded from the sample containing more than 50 gall/1g leaf sample. Number of eriophyid mite galls is negatively correlated with chlorophyll a and chlorophyll b content of bay leaf.

Knowledge Management: Journal