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Research Detail

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M Islam
Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, Bangladesh

A Habib
Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, Bangladesh

S Khan
Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, Bangladesh

S Akter
Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205,

B Goswami
Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205,

H Khan
Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh

TA Banu
Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205,

Twelve varieties of oil seed Brassica spp. were characterized at molecular level using Random Amplified Polymorphic DNA (RAPD) primers to explore genetic diversity and to find out relationship among them. Twelve random primers used in the study generated 94 RAPD fragments and 53 (56.38%) of them were considered as polymorphic indicating high level of polymorphism within the materials. The size of amplified fragments ranged between 300-3000 bp. The values of pair-wise genetic distance ranged from 0.1613 to 0.5543. To find out phylogenetic relationships among the varieties, dendrogram based on Nei’s genetic distance was constructed using Unweighted Pair Group Method of Arithmetic Means (UPGMA) separating the 12 Brassica spp into two major clusters C1 and C2. This result will be useful for designing future breeding programmes for improvement of Brassica varieties.

 

  Mustard; RAPD markers; PCR; Polymorphisms
  Bangladesh Agricultural Research Institute (BARI), Gazipur.
  
  
  Variety and Species
  Mustard

To find out phylogenetic relationships among the varieties, dendrogram based on Nei’s genetic distance was constructed using Unweighted Pair Group Method of Arithmetic Means (UPGMA) separating the 12 Brassica spp into two major clusters C1 and C2.

A total of 12 varieties of Brassica spp. namely Brassica campestris var. BARI Sarisha-1 (Tori-7), Brassica juncea var. BARI Sarisha-2 (Rai-5), Brassica campestris var. BARI Sarisha-6 (Dhali), Brassica campestris var. BARI Sarisha-9 Brassica juncea var., BARI sarisha-10, Brassica juncea var. BARI Sarisha-11, Brassica campestris var. BARI Sarisha-12, Brassica napus var. BARI Sarisha-13, Brassica campestris var. BARI Sarisha-14, Brassica campestris var. BARI Sarisha-15, Brassica juncea var. BARI Sarisha-16 and Brassica campestris var. BARI Sarisha-17 were investigated in this study. These 12 varieties of Brassica were collected from Bangladesh Agricultural Research Institute (BARI), Gazipur. The experiment was performed in the Tissue Culture Laboratory of Bangladesh Council of Scientific and Industrial Research, (BCSIR), Dhaka-1205. To extract genomic DNA, young and actively growing fresh leaves were collected from each of the 12 varieties of Brassica spp. seedlings. Total genomic DNA was extracted by using a modified CTAB method (Doyle and Doyle, 1987). DNA quality was checked by electrophoresis in 1% agarose gel and quantified through a spectrophotometer (Analylikjena, Specord 50, Germany) at 260 nm wave length. To perform RAPD analysis, seventeen decamer primers (Operon Technology, USA and Sigma, USA) were applied of which twelve primers exhibited good quality banding patterns and sufficient variability. These twelve primers were selected for further analysis.

  Bangladesh J. Sci. Ind. Res. 55 (1), 1-8, 2020
  DOI: https://doi.org/10.3329/bjsir.v55i1.46726
Funding Source:
1.   Budget:  
  

The results of the present investigation showed high level of polymorphisms (56.38%) among the twelve varieties of Brassica. Broad range of polymorphism revealed wide variability in Brassica spp. variety. This observed variability would be helpful for future breeding program to improve Brassica varieties in Bangladesh.

  Journal
  

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