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Isolation and Enumeration of Escherichia Coli from Soil and Water

Anindita Bhowmik
Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh

Sunjukta Ahsan*
Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh

Abstract/ Executive Summary:

Majority of the population of Bangladesh depend on tap or surface water as their source of water supply. This study was carried out to examine the microbial quality of both water and soil collected from different places using the multiple tube fermentation technique to determine coliform count by the most probable number (MPN) method in brilliant green lactose broth (BGLB) media.Inoculum from positive tubes of the presumptive test were further transferred on eosinemethylene blue (EMB) and MacConkey agar. The organisms isolated were further characterized using biochemical tests. Out of 93 water samples, 30 (32.26%) indicated the presence of lactose fermenter and gas producer in all 3 tubes of dilution series using inoculum quantities of 1.0, 0.1 and 0.01 ml, whereas out of 85 soil samples, 45 (52.94%) showed acid and gas production in all 3 tubes of dilution series.Among 85 soil samples, 40 samples that contained at least one positive in each dilution series and among 93 water samples, 31 samples that contained at least one positive in each dilution series were further re-identified with biochemical tests.This study showed 30.59% soil isolates and 26.88% water isolates were Escherichia coli which highlighted the fact that both water and soil act as a major reservoir of E.coli, which indicates possible fecal contamination as well as presence of potentially pathogenic E. coli.

Keywords: Escherichia coli, Fecal contamination

Location: Dhaka, Chandpur, Norsingdi, Barisal, Tangail,

Start Date:

End Date:

Program Area: Pest Management

Commodity: Nematodes

Objectives:

E. coli has been used as an indicator of recent fecal contamination and represents a threat to human and environmental health. However, for rapid detection of indicator organisms in drinking water as well as in soil, the most probable number (MPN) method, which is not that common in usage, might be considered. It is actually a qualitative test rather than quantitative indicating only the presence of coliforms, not their numerical presentation.

Methodology:

Sample collection:The soil samples were collected from different places of Bangladesh like Dhaka,Chandpur, Norsingdi, Barisal, Tangail, whereas the water samples include both tap and surface water in which tap water were collected from different lacations of Dhaka City and surface water were collected from different ponds of Dhaka, Chandpur, Tangail and Mymensingh districts. Rivers include Buriganga and Dakatia. Soil samples were collected from 85 different locations in UV-treated plastic bags and 93 water samples were collected in UV treated plastic screw capped bottles and transported to the laboratory within one hour and immediately subjected to microbiological analysis.

Source of Information: Bangladesh J Microbiol, Volume 36, Number 2, December 2019, pp 75-77

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

In this study we have found the presence of E.coli both in tap and surface water which are used for drinking, bathing swimming and for many household purposes. Likewise we have isolated a considerable percentage of E.coli from soil samples collected from different places of Bangladesh.

Knowledge Management: Journal