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Evaluation of Semen Parameters of Brahman Graded Bull Compared to Holstein Graded and Local Bulls Using Computer Assisted Sperm Analyzer

Md. Rafikul Islam
Department of Animal Breeding & Genetics, Bangladesh Agricultural University, Mymensingh, Bangladesh

Syed Sakhawat Husain
Department of Animal Breeding & Genetics, Bangladesh Agricultural University, Mymensingh, Bangladesh

Md. Kutub Uddin Talukder
American Dairy Limited, Gazipur, Dhaka, Bangladesh

Md. Shafiqur Rahman
Department of Livestock Services, Dhaka, Bangladesh

Abstract/ Executive Summary:

An experiment was conducted to characterize, compare, estimate of heritability, and genetic and phenotypic correlations of semen parameters of three genetic groups of bulls. The study was carried out at the Central Cattle Breeding and Dairy Farm, Saver, Dhaka from April to November, 2015. A total of 16 bulls from three genetic groups namely Brahman graded (8), Friesian graded (5) and Local (3) bulls were selected. Motility % and sperm concentration (×10⁶/ml) were observed using Computer Assisted Sperm Analyzer (CASA). Data were analyzed using SAS Computer Package Program, version 9.1.3. Analysis of data showed that, individual genotypes had significant (p<0.05) effect on semen parameters. The maximum sperm concentration (×106/ml) was found as 1516.59±33.07 for Local group (LG), intermediate as 1380.38±19.06 for Brahman groups (BG) and lowest as 1105.03±22.54 in Friesian group (FG), respectively. The season had also significant (p<0.05) effect on semen parameters. The estimated heritability for the semen production traits were medium to high as 0.39 to 0.51, 0.36 to 0.48 and 0.35 to 0.44 for BG, FG and LG, respectively. The strongest positive genetic and phenotypic correlations were noticed between the related semen parameters for all the genetic groups. Through ranking of different traits used in this study for each genotypes it is signified that Brahman genotypes stood 1st position in terms of selection index value which has newly been introduced for beef production in Bangladesh.

Keywords: Bull semen, Heritability, Genetic and phenotypic correlation

Location: The Central Cattle Breeding and Dairy Farm (CCBDF) at Saver, Dhaka, Bangladesh

Start Date: 00-04-2015

End Date: 00-11-2015

Program Area: Variety and Species

Commodity: Bull

Objectives:

To determination and evaluation of semen parameters of Brahman graded bull compared to Holstein graded and Local bulls using Computer Assisted Sperm Analyzer

Methodology:

Research site, selection of breeding bulls and semen collection: The present study was conducted the Central Cattle Breeding and Dairy Farm (CCBDF) at Saver, Dhaka from April to November, 2015. A total of eight Brahman graded bulls of G1 generation (Brahman x Local=50:50) were selected from “Beef cattle development project”. The other experimental bulls were five Friesian graded (Friesian × Local =50:50) and three Local bulls selected from CCBDF. The age of the bulls were determined by the date of birth from the record book maintained by AI Center. The bulls were between 3.11 to 5.5 years in age for Brahman, 4.5 to 6.5 years for Friesian and 4 to 7 years for Local. The body weight and scrotal circumference of bull were 570 to 710 kg and 31.5 to 40.1 cm for Brahman, 550 to 750 kg and 34 to 37.5 cm for Friesian, and 400 to 450 kg and 33.5 to 38 cm for Local, respectively. The semen was collected once a week with two ejaculations during each collection session by means of AV (artificial vaginal method). Before collection all the parts of Artificial Vagina were cleaned, sterilized, assembled properly and the internal temperature was maintained at 42 to 450 C. After collection, semen was placed immediately into water bath at 370 C until going for further handling. A total of 266, 170 and 99 ejaculates were collected from three genetic groups viz. Brahman graded, Friesian graded and Local bulls, respectively. Physical evaluation: The ejaculate volume was recorded by reading the graduated mark of the collection vial in milliliter. The color of semen was recorded as milky and creamy white depending on the thickness and pigments of the semen and was assigned a numerical value 1 for milky white and 2 for creamy white for statistical analysis. The consistency of semen was observed by inclining and moving the semen in collection vial with care. It was recorded and scored in 4 scales viz. 1= thin milky, 2= thick milky, 3= thin creamy and 4= thick creamy. Microscopic evaluation (mass activity, sperm concentration and motility) The mass activity of fresh semen was evaluated using phase contrast microscope (Nikon Eclipse YHDO9628) with 10X zoom and a heated stretching table. One drop of fresh semen was placed on pre-heated table at 370 C and covered with a cover slip. The mass activity was scored from 1-4 scales as follows:
1 = weak motion without forming any wave;
2 = small, slow moving wave;
3 = vigorous movement with moderate rapid waves and eddies and
4 = dense, very rapidly moving waves and eddies.

Source of Information: J Bangladesh Agril Univ 18(2): 442–448, 2020

Article Link (Online Journal): https://doi.org/10.5455/JBAU.81689

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The individual genotypes had significant (p<0.05) effect on different semen parameters. According to the phenotypic value of semen parameters, the local genetic group was found superior to other genotypes but BG stood intermediate position. In seasonal comparison, FG performed better at winter and rainy, but BG performed better in winter and summer and LG performed average all over the seasons. The estimated heritability among three genotypes was medium to high for all the genetics groups. In the present study, most of the estimated heritability from three genetic groups was fairly appreciable. However, the differences between estimates obtained from literature and present study could partly be explained by greater environmental changes in which the individuals were exposed during the study, sample size used and genetic constitution of the population from which samples were picked up. Among semen characteristics, the strongest genetic and phenotypic correlations were found between sperm concentration with semen consistency but most of the favorable genetic and phenotypic correlations was found in BG. On the basis of phenotypic, economic weight value and heritability value, Brahman graded bull got the 1st ranking out of three genotypes. So semen of Brahman graded bull could satisfactorily be used for genetic improvement of cattle in relation to meat production.

Knowledge Management: Journal