Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Evaluation of Jute Leaf as Substitute of Fish Meal in the Diet of Mrigal (cirrhinus Cirrhosus) Fingerlings

K.M.S. Rana
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

P. Biswas
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M.A. Salam
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Abstract/ Executive Summary:

The study was conducted to identify jute leaf powder as an alternate to fish meal in diets of juvenile mrigal (Cirrhinus cirrhosus) for 60 days. Tossa jute (Corchorus olitorius) leaf was selected to utilize this unexplored nutritious resource rather leaving under water for potential pollution. Three isonitrogenous test diets were prepared and applied as treatments (T) in triplicates (R). In control (T₀) dietary inclusion rate of fish meal was 30%, of which 10% was substituted with jute leaf powder in T₁₀ and in T₂₀ replacement was 20%. Mrigal fingerlings (9.38±0.13 cm and 7.94 ±0.26 g) were stocked in nine plastic half drums (0.26 m² each) at 10 fish per drum and fed test diets. Although, growth parameters among the treatments were statistically similar, the highest mean length gain, weight gain, SGR and production were 1.51 (±0.18) cm, 2.96 (±0.13) g, 0.53 (±0.03) %/day and 4084.00 (±50.67) kg ha^-1, respectively in T₁₀. However, significantly higher (P<0.05) survival was found in T₁₀ (93.33%) and T₂₀ (90.00%) compared to T₀ (83.33%). Juveniles in T₁₀ and T₂₀ showed better tolerance to low pH stress than T₀. Water quality parameters were within acceptable range in all the treatments. Moreover, carcass composition of fish was statistically similar among the treatments. Importantly, feed formulation cost was reduced by 3.7% and 20.4% in T₁₀ and T₂₀, respectively compared to T₀. Therefore, the results signify that jute leaf powder could be a promising substitute of fishmeal in mrigal diet without hampering growth along with improved survival and low feed cost.

Keywords: Fish meal, Isonitrogenous, Jute leaf, Mrigal.

Location: Department of Aquaculture, Bangladesh Agricultural University, Mymensingh.

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Fish

Objectives:

To evaluate jute leaf powder as feed ingredient for juvenile mrigal by progressively replacing fishmeal in their diet.

Methodology:

The study was carried out for 60 days in the “BAU Aquaponics Oasis” at the Department of Aquaculture, Bangladesh Agricultural University, Mymensingh. The experimental design comprised of nine well aerated fish holding tanks (each of 0.26 m²), labeled and placed in two rows following complete randomized block design for experimental congeniality. The tanks were prepared by cutting, washing, drying, setting with aerators, filling up with water and covered with net frame to prevent the fish jumping out or predatory animals invasion. Mrigal fingerlings (average size: 9.38±0.13 cm and 7.94 ±0.26 g) were stocked at a density of 10 fingerlings/ 0.26 m² tank after proper acclimatization. Collection and preparation jute leaf powder- The jute leaves were collected from local market. After collection, the leaves were separated from stems, washed with tap water, aerated with electric fan in room condition to evaporate residual moisture and then dried in a homemade dryer at 40°C. When jute leaves became crunchy they were finely ground using blender and then sieved. However, prior to formulating feed, prepared jute leaf powder was analyzed for nutritional profile. Ingredients selection and fish feed formulation- The following ingredients were selected for feed formulation based on their availability, nutrients content and market price. Feeds (test diets) were formulated by emphasizing progressive replacement of fishmeal with jute leaf powder. Three different test diets were formulated by replacing 0, 10 and 20 % of fish meal with jute leaf powder, which were designated as T₀, T₁₀ and T₂₀, respectively. In the control (T₀), dietary inclusion of fishmeal was 30% (but no jute leaf powder), which was substituted with jute leaf powder and subsequently reduced to 27% and 24% in T₁0 and T₂₀, respectively. Inclusion rates of different feed ingredients were determined following Pearson’s technique to prepare isonitrogenous (around 30% protein) test diets. Feeding of fish, sampling and data analysis- The mrigal fingerlings were fed with the experimental diets at the rate of 10% of their body weight twice daily. The uneaten feed and faces were removed from the tanks with 25% daily water exchange through siphoning and the entire water changed fortnightly. Moreover, fish were sampled fortnightly throughout the study period in order to observe their growth response to the test diets by calculating the growth parameters such as length gain (cm), weight gain (g), percent weight gain, specific growth rate (SGR, % day^-1), food conversion ratio (FCR), survival rate (%) and fish production (kg ha^-1). Fish carcass profile was also determined following the standard procedure of AOAC (1990). Besides, water quality parameters such as dissolved oxygen (mg L^-1), water temperature ( °C), pH, ammonia and nitrite contents were measured using portable DO meter, thermometer, pH meter and ammonia testing kits, respectively. However, collected data were subjected to one-way ANOVA for statistical analysis (Snedecor and Cochran, 1994). The least significant difference was used for comparison of the mean values ascertained from different treatments. Low pH stress test Tolerance of the test fish against low pH stressor (pH 3) was also observed to determine the effect (if any) of the test diets on fish fitness. Therefore, after the feeding trial, two fish from each rearing tank (6 fish per treatment) were randomly selected and transferred to a 20 L bucket containing water having pH 3. Deep tube-well water was strongly aerated for 24 h and gradually mixed with nitric acid (HNO₃) to avail this low pH water (pH 3). The tanks for stress test were equipped with continuous aeration and kept under ambient temperature. The passing of time to reach 50% mortality was calculated as median lethal time (LT₅₀).

Source of Information: Int. J. Agril. Res. Innov. Tech. 10(1): 117-122, June 2020

Article Link (Online Journal): DOI: https://doi.org/10.3329/ijarit.v10i1.48103

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Reducing feed cost has always been a crucial issue in aquaculture. The current venture promotes the dietary inclusion of jute leaf powder in order to cost effectively replace fish meal in the diets of mrigal fingerlings without adversely affecting fish growth and carcass composition at improved survival.

Knowledge Management: Journal