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Investigation of Self-incompatibility in Mustard Through Seed Set Analysis

Amirul Bahrain
Upazila Agriculture Officer
Department of Agriculture Extension, Derai, Sunamganj, Bangladesh

Md. Ashikul Islam
Senior scientific officer
Spices Research Centre, BARI, Shibganj, Bogra, Bangladesh

Afiza Kakon
Student
Hajee Mohammad Danesh Science and Technology University, Dinajpur, Banhladesh.

Mst. Shamsun Naher
Scientific officer
Spices Research Centre, BARI, Shibganj, Bogra, Bangladesh

Md. Motaher Hossain
Scientific officer
Spices Research Centre, BARI, Shibganj, Bogra, Bangladesh

Abstract/ Executive Summary:

Twenty genotypes of mustard were grown and investigated for self-incompatibility at the Bangabandhu Sheikh MujiburRahman Agricultural University, Gazipur 1703, Bangladesh during rabi season of 1997-98. Seed set analysis were used to identify the self-incompatibility mechanism which indicated that the genotype Safal, Binasarisha-3, SS-75, Vardon, Na-8509, Dhali, Daulat, Seeta, Kranti, Rai-5, Nap-3142, Jun-3018, Jun-4008, Nap-3015 and PT-30 were classed as self-compatible; while intermediate fertility were observed for TS-72, PT-303, M-27 and Tori-7 as those four genotypes showed more than 10 percent but less than 50 percent seed set ratio. Self-incompatibility study indicated that none of the genotypes under study was found full self-incompatible

Keywords: Investigation, Self-incompatibility, Mustard, Seed set analysis

Location: Bangabandhu SheikhMujiburRahman Agricultural University, Gazipur-1703, Bangladesh

Start Date: 10-11-1997

End Date: 20-02-1998

Program Area: Variety and Species

Commodity: Mustard

Objectives:

Estimate the nature of self-incompatibility mechanism in twenty local mustard genotypes.

Methodology:

The experiment was conducted at the Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, during the winter season of 1997-98. The seeds of twenty mustard genotypes (13 cultivars and 7 advanced lines) were collected from Oil Seeds Research Center of Bangladesh Agricultural Research Institute Joydebpur, Gazipur . The genotypes were Vardon. Seeta, Daulat, Dhali, Kranti, Nap-8509, Nap-3142, Rai-5, Tori-7, PT-30, PT -303, Jun-4008, Jun-3018, Jun-2592, TS-72 (Kallanya), SS-75 onali Sorisha), M-27, Binasarisha-1 (Safal), Binasarisha-3. The seed of 20 genotypes were sown in the experimental field on 10 November, 1997 and single row of 5m each constitute the experimental plot. The row spacing was 50 cm having plant spacing 30 cm with in the row. Fertilizers were applied @ 120:80:60:40:4 kg ha^-1of N, P2O5, K20, S and Zn respectively. Two to three seeds of the genotype were sown per hill. Seeds were planted at 0.2-0.5 cm depth. The seedlings emerged three to four days after sowing. Irrigation were applied before flowering and grain filling stages. The data were collected from seed set analysis method. Seed set analysis were used to identify the self-incompatibility mechanism. Hand pollination method was used in this experiment. To determine the incompatibility of mustard, selfing was done to compare the difference of fertility between flowering stage and bud stage. Data were collected through i) seed set analysis method and ii) pollen germination and pollen tube growth method.

Pollen germination and pollen tube growth

Fluorescence microscopy (8,10,22) was employed to test the self-incompatibility mechanism by observing pollen germination on the stigma and pollen tube growth in the style of each cultivar. Three type of pollination viz bud self, bloom self and crosspollination were performed on these cultivars. Ten flowers were pollinated in each treatment. Cross pollination was done by using pollen from PT 303. However,PT 303 was crossed with SS 75 as male parent. The pollinated flowers were removed from the plants after 24 hours of pollination.

Source of Information: Journal of Scientific Achievements, Volume 2,Issue 5,May 2017,Page:12–17

Article Link (Online Journal): http://jsciachv.sinaweb.net/article_80727.html

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Self-incompatibility study indicated that none of the genotypes under study was found full self-incompatible. For hybrid seed production in mustard using self-incompatible, an extensive collection program should be undertaken for local and exotic genotypes. Then testing for self-incompatibility should be made through seed set analysis to identify desired incompatible but cross compatible types.

Knowledge Management: Journal