The present checklist is prepared on the basis mainly of the reviews of published literatures, several recent catalogues on spiders (Platnick 2012,World Spider Catalog, version 19.5, 2019,Proszynski 1990, Mikhailov1997, Logunov and Marusik 2000), Checklists and Monographs (Biswas et al.1993, Okumaet al.1993, Begum and Biswas 1997, Sadana and Molour 1997, Keswaniet al. 2012), Fauna volumes (Pocock 1900, Tikader 1980, 2082, Chen and Zhang 1991, Kim and Lee 2012, Gajbe 2006, Majumder and Tikader 1991. Zhuet al. 2003, Yinet al.1997, Songet al. 1999, Zhu 1998, Zhuetal. 2003, Barrion and Litsinger 1995, Biswas 2009 ), recent spider books (Tikader 1987 Koh1989, Yaginuma 1986, Zhao1993, Roberts1995, Saha and Raychaudhuri, 2003, 2004, 2007 a,b,c,d, Benjamin 2016, Sebastian and Peter 2009) and many papers published in national and international journals and computer databases (e.g.Proszynski’s Catalogue of jumping spiders of the world). Beside these, there are some identified species those are not included in this list as these are not published as yet. All those are at present in the collection of the Department of Zoology, Khulna Govt. Womens’ College, Khulna, Bangladesh and will include in future revision of the checklist.These specimens after description will be deposited to the Museum of the Department of Zoology, University of Dhaka, Dhaka-1000, Bangladesh in due course of time. The present checklist is made in a taxonomic order of classification of spiders viz., Infra-order, family, genera and species. For each of the families, of this checklist,the information are given in the following sequence-name of the species, author, year, sex and possibly distribution. As a first preparation, there may be some lacking of information from the old literatures. So, in those cases no distribution and other information were cited. It will be completed in future.Consideringthe study of the students and researchers of the future generation in the field of arachnology in Bangladesh, a brief diagnosis for each of the families are given along with the list of genera and species. Therefore, the present checklist may be a taxonomic tool in the history of the study of arachnids (spiders) of Bangladesh.