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Larval Locomotion Behavior in Three Species of Drosophila

Mohammad Shamimul Alam
Genetics and Molecular Biology Laboratory, Department of Zoology, University of Dhaka, Bangladesh

Farjana Sultana Poly
Genetics and Molecular Biology Laboratory, Department of Zoology, University of Dhaka, Bangladesh

Sadniman Rahman
Genetics and Molecular Biology Laboratory, Department of Zoology, University of Dhaka, Bangladesh

Rowshan Ara Begum
Genetics and Molecular Biology Laboratory, Department of Zoology, University of Dhaka, Bangladesh

Abstract/ Executive Summary:

Locomotion behavior of Drosophilamelanogaster, D. ananassaeand D. bipectinata was analyzed to know the pattern of locomotion for the respective species and to find interspecific relationship among them. Among the three species,D. bipectinata larvae passed the highest distance. In terms of turning per minute, D. melanogaster differed significantly from other species. Average bending number was also highest in this strain. The relationship of the three species has been discussed in terms of locomotion parameters and available phylogenetic trees relevant to these species. Locomotion is an integral component of most animal behaviors. They rely on this behavior for searching food,mates and escaping from predators and respond to different stresses (Heckscher et al. 2012). Thus, understanding this behavior is helpful. As a fantastic model organism, Drosophila is being utilized in different behavioral studies (Reiter et al. 2001, Mcguire et al. 2005). Inter and intra-population digging behavior of larva have also been studied in Drosophila (Godoy-Herrera 1977). Larval foraging behavior, which includes locomotion, varies from species to species reflecting that this behavior can be differed from species to species within a same group (Godoy-Herrera and Connolly 2007). The richness in Drosophila species diversity also provides a great opportunity for extensive study (Singh 2015). Ten Drosophila species have so far been reported from Bangladesh (Sultana 1998). Among them, D. melanogaster, D. bipectinata and D. ananassae are commonly found. Previous work regarding locomotion behavior has been done on these species separately, not in the same experimental set-up (Green et al. 1983, Bauer and Sokolowski 1984). These three species have been collected, cultured and identified to explore larval locomotion behavior.

Keywords: Larva, Locomotion, Behavior, Drosophila species

Location: Dohar, Dhaka, Satchari, Sylhet and Hiron Point, Sundarbans

Start Date:

End Date:

Program Area: Pest Management

Commodity: Insects

Objectives:

D. melanogaster, D. bipectinata and D. ananassae are commonly found. Previous work regarding locomotion behavior has been done on these species separately, not in the same experimental set-up (Green et al. 1983, Bauer and Sokolowski 1984). These three species have been collected, cultured and identified to explore larval locomotion behavior.

Methodology:

Procedure of the study: Adult flies collected from different places of Bangladesh, namely Dohar, Dhaka (23°37'06.5"N 90°07'10.1"E), Satchari, Sylhet (24°07'28.0"N 91°26'41.5"E) and Hiron Point, Sundarbans (21°48'58.0"N 89°27'46.6"E) were maintained on standard food. Three species of Drosophila were identified by following the established protocol (Markow and O’Grady 2005). Fly strains collected from Dohar, Hiron Point and Satchori were identified and labeled as D. bipectinata (DR), D. melanogaster (HP) and D. ananassae (SC), respectively. Third instar larvae of all the three strains were placed in the middle of 2% agar plate over a background paper where each grid was of 0.5 cm. For each larva, video was captured for about 1 minute. For each species of Drosophila, the whole procedure was repeated for at least 20 times. When a larva changed its direction of movement by less than 90 degree and more than 90 degrees, it was considered as turning and bending, respectively. The number of grids it crossed revealed the distance traveled. Data were analyzed by using Microsoft excel. Pattern of larval locomotion in the three strains of Drosophila species: Three major activities during larval locomotion, namely distance traveled, turning and bending were calculated and compared among these three strains of the Drosophila species.

Source of Information: Bangladesh J. Zool. 47(2): 367-371, 2019 ISSN: 0304-9027 (print) 2408-8455 (online)

Article Link (Online Journal): DOI: https://doi.org/10.3329/bjz.v47i2.44347

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Overall, present observations confirm that larval locomotion behaviors differ from species to species in the Drosophila species. Besides, the behavioral differences can be related to their phylogenetic relationship as well. Thus,analyzing different locomotion behavior would be helpful for species identification, differentiation as well as studying evolution. Present work has provided baseline data that will be valuable in this regard. Further extensive study is needed for better understanding.

Knowledge Management: Journal