The experiment was conducted at the Poultry Research Shed of Chattogram Veterinary and Animal Sciences University (CVASU), and all the experimental bird’s care, handling, and management of this study were approved by the Animal Ethics Committees of CVASU, Bangladesh [Approval No. CVASU/Dir(R&E). EC/2019/94(4)]. All expenditures, such as chick cost, feed ingredients, and others were recorded during the experimental period. Two birds per replicate cage were selected randomly and then blood samples were taken for serum analysis [triglyceride (TG), glucose, total protein (TP), albumin (Alb), uric acid, and creatinine] at day 33. These birds were then weighed and killed humanely to record the carcass yield traits (breast weight, thigh weight, wing weight, shank weight, drumstick weight, neck weight, and abdominal fat content). Right tibial samples were also collected to assess the leg bone traits (bone weight, length, width, head width, Ca% and P%). Formulated feed samples were also collected before providing the birds to determine the chemical composition of the test diets. Samples of about 500 gm from each of the starter and finisher diets were taken and ground by a coffee grinder. After that, the samples were sent to the Poultry Research and Training Center lab for testing dry matter (DM%), moisture %, crude protein (CP%), crude fiber (CF%), ether extract (EE%), ash, calcium (Ca%), and phosphorus (P%) using standard laboratory procedure [19]. Metabolizable energy (ME) was estimated indirectly on the basis of true metabolizable energy (TME) contents of the feed samples, assuming that TME was 8% higher than the ME, as it is reported that TME is 5–10% higher than ME [20]. On day 33, two birds per replicate were selected randomly for collecting blood samples for assessing blood serum profiles. Blood samples were collected from the wing veins into sterile vacutainers (4 ml) without anticoagulant. Collected blood samples were sent to the Department of Physiology, Biochemistry, and Pharmacology, CVASU, for measuring blood metabolites. Samples were kept undis- turbed in a vertical position (using test tube rack) for 4 h into the refrigerator to allow the separation of serum. Then, the samples were centrifuged at 3,000 rpm for 10 min to separate the serum. Afterward, the serum samples from each tube (accumulated as supernatant) were collected carefully by using a micropipette and taken into the sterile Eppendorf tube (2 ml) followed by stored at −20°C till analysis. Glucose, TP, Alb, TG, creatinine, and uric acid were analyzed using standard kits (Randox Laboratories Ltd., UK) and automatic analyzer (Humalyzer 300, Merck, Germany, semi-automated Benchtop chemistry photometer) according to the manufacturers’ instructions. After collecting blood samples birds were then weighed and slaughtered humanely to collect different meat parts on the same day. After slaughtering, the birds were bled properly followed by skinning. Afterward, different meat yield parameters, such as carcass weight, dressed weight, weights of different meat cuts (neck, thigh, wings, breast, back, drumstick, shank and abdominal fat) were recorded. Right tibia bone samples were also collected on the last day of the trial period to assess the leg bone quality of broiler chicken. Collected bones were processed by boiling 10 min in deionized water to facilitate the removal of the attached soft tissues and defatted. After that, bone length and head width were taken by using digital calipers (Insize, Japan) and the weight was recorded by digital balance. After that, the bone samples were assessed for bone mineral con- centration, particularly Ca and P. At first, the samples were dried properly and then ground and later put the sample on a Muffle Furnace at a temperature of 600°C for 4 h to make ash. After taking bone ash weight, the samples later analyzed for Ca and P by atomic absorption and spectrophotometry. Minitab statistical software was used to analyze all collected data of this study (Minitab Version 16, 2000). The data were subjected to one-way analysis of variance for CRD and tested for significance between the dietary treatment means by using Duncan’s Multiple Range Test (DMRT), and statistical significance was considered at p ≤ 0.05.