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Effect of Nacl on Germination and Seedling Growth of Some Cotton Genotypes

Kamelia Mondal
Agrotechnology Discipline, Khulna University, Khulna- 9208, Bangladesh

Joyanti Ray
Agrotechnology Discipline, Khulna University, Khulna- 9208, Bangladesh

Md Yasin Ali
Agrotechnology Discipline, Khulna University, Khulna- 9208, Bangladesh

Abstract/ Executive Summary:

Salinity is an emerging problem among the abiotic stresses of plants in Bangladesh. One easy way to cope with the problem of salinity is to exploit the genetic potential of plants for their adaptability to adverse soil conditions. Salt tolerance improvement might be achieved through selection from already existing germplasm which can be done by quick method particularly in early growth stages of crops. A laboratory experiment was conducted to determine the effect of salinity on germination and the seedling growth of five cotton genotypes. Two factors include genotypes (CB-9, CB-10, CB-12, CB-14 and CB-15) and salt solution of different concentrations (control -0 dS m^-1, 4 dS m^-1, 8 dS m^-1, 12 dS m^-1 and 16 dS m^-1) were used. Relative to control treatment, germination energy and germination capacity at 16 dS m^-1 was maximum in CB-12 (0.72 and 0.77 respectively) whereas germination percentage (0.99) was maximum in CB-9 and the relative value of all the above parameters was minimum in CB-15 (0.02, 0.13, 0.41 and 0.09, respectively). In case of seedling growth parameters, shoot length, seedling vigor index and dry weight at 16 dS m-1 were maximum in CB-9 (0.67, 0.64 and 0.77 respectively) relative to control whereas the relative value of all those parameters was minimum in CB-15 (0.5, 0.17, 0.31 and 0.59 respectively). Considering all germination and seedling growth parameters, CB-9 was supposed to be superior genotype followed by CB-12, CB-10 and CB-14 whereas genotype CB-15 was more susceptible than other genotypes against salinity stress.

Keywords: Cotton, Germination, Seedling growth, Salinity

Location: Agronomy Laboratory of Agrotechnology Discipline, Khulna University, Khulna, Bangladesh

Start Date: 00-07-2019

End Date: 00-08-2019

Program Area: Crop-Soil-Water Management

Commodity: Soil salinity, Cotton

Objectives:

To examine the effect of salinity (NaCl) on germination and seedling growth of some cotton genotypes.

Methodology:

The experiment was conducted in Agronomy Laboratory of Agrotechnology Discipline, Khulna University, Khulna, Bangladesh from 24 July to 12 August, 2019 to determine the effect of salinity on germination and the seedling growth of five cotton genotypes. The experiment was conducted by using two factorial completely randomized designs (CRD) with four replications. The treatments were genotypes (CB-9, CB-10, CB-12, CB- 14 and CB-15) and salinity levels (T₁= Control (0 dS m^-1), T₂= 4 dS m^-1, T₃= 8 dS m^-1, T₄= 12 dS m^-1 and T₅= 16 dS m^-1). Seeds of all the genotypes were collected from Cotton Research, Training and Seed Multiplication Firm, Sreepur, Gazipur. Glass petridishes with a tight fitting lid were used in conducting this experiment. Filter papers were used as a matrix for seed germination. Three filter papers were soaked with respective salt solutions (Control, 4, 8, 12 and 16 dS m^-1 respectively). For preparing different salt solution, 1000 ml distilled water was kept in a beaker and then respective amount of salt (NaCl) was gradually added to this water. To assure the concentrations of solution, an electrical conductivity meter was used to measure the salinity levels. Before placing the seeds to petridishes, seeds were treated with Vitavex 200 @ 3 gm/ kg seed and kept them in a air tight packet for 24 hours. The number of petridishes that used in laboratory were = {5 (variety) × 5 (treatments) × 4 (replication)} = 100 A seed has considered germinated at the emergence of the radical (2 mm) (Chartzoulakis and Klapaki, 2000). After germination, seedlings were watered as and when necessary. The data were taken on following parameters- (i) germination energy [% of seeds germinated at 72 h]; (ii) germination capacity [% of seeds germination at 168 h] (Bam et. al., 2006); (iii) germination percentage [(No. of seed germinated/total no. of seed in petridish) ×100]; (iv) germination speed (%) [(No. of seed germinated at 72 h/ No. of seed germinated at 168 h) ×100]; (v) root length (cm); (vi) shoot length (cm); (vii) seedling vigor index [(average shoot length+ average root length) × germination percentage] (Podder et al., 2020) and (viii) dry weight of seedling (mg) [after 10 days of setting the seed for germination, seedlings of each petridish were dried in oven at 600C until a fixed weight which was measured by an electrical balance]. Relative performance was measured by using the following formula- Relative performance = Variable measured under stress condition/ Variable measured under normal condition Then the recorded data were analyzed with the help of statistical package program MSTAT-C, Microsoft Excel and the mean differences were adjusted with Duncan’s New Multiple Ranges Test at 5% level of significance (Gomez and Gomez, 1984).

Source of Information: Res. Agric., Livest. Fish.7(2): 199-207, August 2020

Article Link (Online Journal): DOI: https://doi.org/10.3329/ralf.v7i2.48860

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Considering germination and seedling growth parameters, CB-9 was supposed to be superior genotype followed by CB-12, CB-10 and CB-14 whereas genotype CB-15 was more susceptible than other genotypes against salinity stress. Similar study should be conducted in the field to better understand the effects of salinity stress on growth and yield performance of cotton.

Knowledge Management: Journal