TM Rahsin Kabir
Assistant Commissioner and Executive Magistrate, Present : DC Office Lalmonirhat, Bangladesh
Nahid Nawrin Sultana
Veterinary Surgeon, Department of Livestock Services, Ministry of Fisheries and Livestock, People's Republic of Bangladesh, Bangladesh
Tangila Ferdausi
Veterinary Surgeon, Department of Livestock Services, Ministry of Fisheries and Livestock, People's Republic of Bangladesh, Bangladesh
Muhammad Rakibul Hasan
Livestock Extension Officer, National Agricultural Technology Programme (NATP; Phase II), Department of Livestock Services, Ministry of Fisheries and Livestock, People's Republic of Bangladesh, Bangladesh
Nayamul Bashar
Major, Bangladesh Army, Bangladesh
Nazim Ahmad
Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
Shoti, Broiler, Growth performance, Serum, Blood parameters
Department of Physiology, Bangladesh Agricultural University, Mymensingh.
Animal Health and Management
The experiment was conducted to investigate the effect of Shoti in Cobb -500 Broiler chicks during February 2013 to March 2013 in the department of Physiology, Bangladesh Agricultural University, Mymensingh. A 22 days feeding study was carried out to assess the effects of raw Curcuma zedoaria (Shoti) meal upon overall growth, hematological (total erythrocyte count, hemoglobin concentration, erythrocyte sedimentation rate, packed cell volume) and biochemical (cholesterol, triglyceride, high density lipoprotein, low density lipoprotein) parameters. Shoti meal preparation- Curcuma zedoaria (Shoti) was collected from Bangladesh Agricultural University (BAU) campus and were cleaned and chopped into pieces, sun dried and pulverized in a flour meal. The procedure followed was exactly as that of (Latif et al., 1979). The crude protein content of the shoti meal thus prepared was found to be 83 gm/ Kg feed. Maize starch, corn oil, soybean meal, amino acids, minerals and vitamins were bought locally and were of general purpose grade. Test and control diets were formulated by substitution of maize starch.
Experimental design-Twenty broiler chicks, aging 16 days were randomly divided into five equal groups (n=4). Two groups were considered as control (Protein and Non protein) and rest were considered as treated groups. Body weight of individual bird was recorded at the beginning and at the end of experiment. The birds were sacrificed to collect blood sample for hematological and biochemical analysis. Live weight, weight of skin, dressed carcass, legs, liver, intestine with its length were recorded. Preparation of the experimental house and equipment- Experimental house was brushed, sweeping properly and cleaned with tap water. After washing with clean water, the pens were disinfected by using chlorine solution (50 ppm). The room was left vacant for 7 days. Later, it was again disinfected with Timsen solution (1 gm/litre water) left to dry up properly. During this time, all the feederers, waterers and other necessary equipment were properly cleaned, washed and disinfected with Finis solution and dried before use. Management- The commercial management procedures were followed during the whole experimental period. Fresh and dried rice husk was used as a litter at a depth of 2 cm. The litter was stirred three times a week to prevent cake formation. As per need old litter material was changed using new rice husk to prevent fungal or coccidian invasion. Each pen was 2.5 ft x 2 ft and was allotted for four birds. Therefore, the space given for each bird was 1 square ft. The birds were always exposed to a continuous lighting of 12 hours a day. During night electric bulbs were used to provide necessary light. In order to maintain required temperature and humidity inside the pens, all the windows of the laboratory were kept open during day and during night a 100 watt bulb was provided as a source of heat. One trough feeder and one round waterier were provided for each replicate pen. Proper hygienic and sanitation programs were followed during the experimental period. To prevent the outbreak of disease strict biosecurity was maintained during the experimental period by visitors restriction and cleaning. Measurement of body weight- The body weight of each bird was measured with the help of electric balance on the 16 of age (0 day of experiment) and at the end of the experiment. The birds were sacrificed, processed and then weights (Live weight, weights of dressed carcass, liver, skin, legs, intestine) were taken by electric balance to study the meat yield and intestinal length was also recorded. Collection of blood samples Blood from each bird was collected at slaughter. A number of sterile test tubes containing anticoagulant (3.8% Trisodium citrate solution) at a ratio of 1:10 were taken. About 5.0 ml of blood was collected for hematological studies. The hematological studies were performed with in two hours of collection. Preparation of serum samples- About 3 ml of blood was collected in the sterile glass test tube. The blood containing tubes were placed in a slanting position at room temperature for clotting. Then the tubes were incubated overnight in refrigerator (40C) and the serum was collected. The sample was centrifuged at 1000 rpm for 15 minutes to have a more clear serum. The serum samples were separated and stored at -200C till analysis. Statistical analysis All data were expressed as mean ± SE, and differences among the groups of birds were compared using one-way ANOVA with post-hoc Duncans test. Paired t- tests were used to compare pre-treatment and post treatment value of different groups. Statistical significance was set at p < 0.05. Statistical analysis was performed using SPSS software version 17 (SPSS Inc., Chicago, IL, USA).
Res. Agric., Livest. Fish.7(2): 293-301, August 2020
Journal