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First Report of White Mould Caused by Sclerotinia Sclerotiorum on Pea in Bangladesh

Md. Rabiul Islam
Department of Plant Pathology, Bangabandhu Sheikh MujiburRahman Agricultural University, Gazipur 1706, Bangladesh

Ananya Prova
Department of Plant Pathology, EXIM Bank Agricultural University, Chapainawabganj 6300, Bangladesh

Abdul Mannan Akanda
Department of Plant Pathology, Bangabandhu Sheikh MujiburRahman Agricultural University, Gazipur 1706, Bangladesh

Md. Motaher Hossain
Department of Plant Pathology, Bangabandhu Sheikh MujiburRahman Agricultural University, Gazipur 1706, Bangladesh

Abstract/ Executive Summary:

Pea (Pisum sativum L.) is one of the important winter-season legume crops in Bangladesh. In January 2018, farmers’ fields in Chapainawabganj district were surveyed for pea diseases.Tan to light brown blighted stems and pods were detected in 2.6% of plants with an average disease severity rating of 3.7 (Little and Hills1978). Prominent dark sclerotia (3.5–14.8 ×1.9–5.3 mm) were found on the surface of the infected tissues or were embedded within the tissues. Surface-sterilized symptomatic tissues were placed on potato dextrose agar (PDA)and incubated at 25 °C for 4 days. A white appressed mycelial growth radiated over the entire plate, with a ring of sclerotia forming on the edge of the plate by 7 days. Pathogenicity of the isolate was tested on 3-week-old pea plants (cv. BARI Motor shuti-3) by placing a mycelial plug of the fungus on to the superficially wounded stem above the soil (Prova et al.2018).

Keywords: Blighted stems and pods, Disease severity, Dark sclerotia, White appressed mycelia, Necrotic lesions

Location: Farmers’ fields in Chapainawabganj district

Start Date: 00-01-2018

End Date:

Program Area: Pest Management

Commodity: Diseases, Pea

Objectives:

In January 2018, farmers’ fields in Chapainawabganj district were surveyed for pea diseases.

Methodology:

Surface-sterilized symptomatic tissues were placed on potato dextrose agar (PDA)and incubated at 25 °C for 4 days. A White appressed mycelial growth radiated over the entire plate, with a ring of sclerotia forming on the edge of the plate by 7 days. Pathogenicity of the isolate was tested on 3-week-old pea plants (cv. BARI Motor shuti-3) by placing a mycelial plug of the fungus on to the superficially wounded stem above the soil (Prova et al.2018). Plants were incubated in a moist chamber for 2 days prior to being transferred to a greenhouse. Necrotic lesions appeared on the stems by 4–5 days after inoculation. The pathogen was re-isolated and the re-isolated fungal cultures appeared morphologically to be Sclerotinia sclerotiorum (Lib.) de Bary. Identification of the fungus was further con-firmed by extracting DNA from the mycelium of three representative cultures. The DNA samples were subjected to PCR using ITS (ITS4, 5′-TCCTCCGCTTATTGATATGC-3′;ITS1, 5′- TCCGTAGGTGAACC TGCGG-3′) primers, which produced 572-bp amplicons that were purified and sequenced using the same primers (Prova et al.2017).

Source of Information: Journal of Plant Pathology (2020) 102:941

Article Link (Online Journal): https://doi.org/10.1007/s42161-020-00518-1

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The best BLASThits for the ITS region (GenBank accession No. MN216247) had 100% identity to strains of S. sclerotiorum. In Bangladesh, S. sclerotiorum has been found on different hosts (Prova et al.2018) but, to our knowledge, this is the first evidence of pea infection in the field.

Knowledge Management: Journal