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Effect of BAU-biofungicide and Plant Extracts Against Root-knot (meloidogyne Javanica) of Okra

S. M. E. Hassan
1Department of Plant Pathology, Hajee Mohammad Danesh Science and Technology University, Dinajpur (5200), Bangladesh.

S. M. M. Hossain
Department of Plant Pathology, Hajee Mohammad Danesh Science and Technology University, Dinajpur (5200), Bangladesh.

A. K. Azad
Department of Genetics and Plant Breeding, Department of Crop Botany, 4Department of Entomology, Hajee Mohammad Danesh Science and Technology University, Dinajpur (5200), Bangladesh.

M. A. Hasan
Department of Crop Botany, ajee Mohammad Danesh Science and Technology University, Dinajpur (5200), Bangladesh.

H. F. El Taj
4Department of Entomology, Hajee Mohammad Danesh Science and Technology University, Dinajpur (5200), Bangladesh.

M. A.Hossain*
Department of Entomology, Hajee Mohammad Danesh Science and Technology University, Dinajpur (5200), Bangladesh.

Abstract/ Executive Summary:

The pot experiments were conducted from October 2009 to March 2010 in the Department of Plant Pathology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh to study the effect of BAU-Biofungicide (Trichoderma spp.), Bermuda grass (Cynodon dactylon), Water spinach (Ipomoea aquatica) and Ivy leaf morninglory (Ipomoea hederacea) against root-knot of okra caused by Meloidogyne javanica using seed treatment and soil drenching methods. Among the treatments, BAU-Biofungicide and Bermuda grass leaf extract (S) gave superior result as it had increased length of shoot and root, weight of shoot and root, number of fruit and weight of fruit plant-1 correspondingly with the lowest galling incidence in okra in all methods. Better effect on plant growth characters with lower galling incidence and development of the nematode was observed with Water spinach extract (S) and Ivy leaf morninglory extract (S) compared to control. Lower effect of plant growth character was found with extract (S) of Ivy leaf morninglory followed by Water spinach leaf extract (S).

Keywords: BAU-Biofungicide, Plant extracts, Root-knot (Meloidogyne javanica), Okra

Location: October 2009 to March 2010 in the Department of Plant Pathology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.

Start Date: 00-10-2009

End Date: 00-03-2010

Program Area: Pest Management

Commodity: Fungicide, Extract (plant, seed)

Objectives:

this experiment has been carried out with BAU-Biofungicide, standard leaf extract of Bermuda grass (Cynodon dactylon), Water spinach (Ipomoea aquatica) and Ivy leaf morninglory (Ipomoea hederocea) to evaluate the efficacy and superiority of BAU-Biofungicide and plant extract against root-knot of okra caused by Meloidogyne javanica.

Methodology:

The pot experiments were conducted from October 2009 to March 2010 in the Department of Plant Pathology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh. Two sets of pot experiments namely; seed treatment and soil drenching with BAU-Biofungicide and plant extracts on growth of 60, 90 and 120 days old okra plant inoculated at 30 days of growth stage with M. javanicabefore 7 days of drenching. BAU-Biofungicide, standard aqueous leaf extracts of Bermuda grass (Cynodon dactylon), Water spinach (Ipomoea aquatica) and Ivy leaf morninglory (Ipomoea hed-eracea) were used in the following treatments:T0 = Control (without extract/ BAU-Biofungicide) T1 = BAU-Biofungicide solution (2%), T2 = Bermuda grass (Cynodon dactylon) extract (S), T3 = Water spinach (Ipomoea aquatica) leaf extract (S), T4 = Ivy leaf morninglory (Ipomoea hederacea) leaf extract (S) arranged in completely randomized design.Sandy loam soil, sand and well decomposed cowdung were mixed properly at the ratio of 2:2:1 followed by sterilization with formalin at the rate of 3% ft-3 soil. After 72 hours, the sterilized soil was exposed to air-drying for 48 hours. Earthen pots (30 cm diameters) were washed with water and sterilized with formalin, then filled with 5 kg sterilized dried soil. Disease free okra seeds cv. BARI-1 were surface sterilized with low concentration of mercuric chloride solution (0.001%) for 1 minute followed by washing thrice with water. Petridish was taken and surface sterilized with methylated spirit before placing seeds for treating with BAU-Biofungicide @ 1:40 w w-1 (BAU-Biofungicide: seed) collected from Disease Resistance Laboratory, Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh. The seeds were thoroughly mixed with the BAU-Biofungicides by shaking the Petridish and placed for shade drying, followed by sowing in the pot on the same day in the afternoon. Leaf (20 g) of Bermuda grass was collected and macerated in an electric blender and soaked separately in l00 ml distilled water in several conical flasks. The filtrate collected was considered as standard concentration of Bermuda grass extract. The same procedure was followed for the preparation of leaf extracts of Water spinach and Ivy leaf morning glory. The seeds were thoroughly soaked in the standard Bermuda grass extract (30 ml) for 30 minutes @ 1:10 w w-1 (extract : seed). Seed treatments and sowing were followed as before. Soil drenching was done following normal procedure. Egg masses were collected from the brinjal roots which was inoculated with a single egg mass of M. javanica before the experiment. Ten reddish brown mature egg masses pot-1 were placed around the both sides of plant root at 2.5 cm deep, five egg masses on each side of the plant. Inoculation was done on the 30 days old seedlings in pots. After 30, 60 and 90 days of inoculation, data on the length of shoot (cm), length of root (cm), weight of shoot (g), weight of root (g), number of galls g^-1 of root, number of fruits plant^-1, weight of fruits plant^-1. The recorded parameters were analyzed by F-test for the treatment means and replication means using Duncan’s New Multiple Range Test at 0.05 level.

Source of Information: International Journal of Bio-resource and Stress Management 2011, 2(4):432-436

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Seed treatment and soil drenching of BAU-biofungicide solution (2%) as well as Bermuda grass leaf extract recorded the highest shoot and root length as well as weight, number of galls g-1, number of fruit plant-1 and weight of fruit (g) plant-1. Therefore, BAU-biofungicide solution (2%) and Bermuda grass leaf extract may be recommended for the control of root knot of Okra.

Knowledge Management: Journal