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Mycoflora Associated with Symptomatic Leaves of Rauvolfia Serpentina (l.) Benth. Ex Kurz. in Bangladesh

Zuhra Yasmin
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Shamim Shamsi
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Abstract/ Executive Summary:

Fifteen fungal species representing 12 genera were found to be associated with leaf spot and anthracnose diseases of Rauvolfia serpentina (L.) Benth. ex Kurz. which were collected from different habitats of Bangladesh. Fungi were isolated following ‘tissue planting’ and ‘blotter’ method. The isolated fungi were Alternaria alternata (Fr.) Keissler, Aspergillus flavus Link ex Fr., A. niger van Tieghm, Colletotrichum gloeosporioides (Penz.) Sacc., Curvularia lunata (Wakker) Boedijn, Fusarium sp.1. Fusarium sp. 2, Macrophoma sp., Nigrospora sphaerica (Sacc.) Mason, Penicillium digitatum Saccardo, Penicillium italicum Whemer, Pestalotiopsis guepinii (Desm.) Stay, Pseudocercospora libenbergii (Syd.) Deighton, Rhizopus stolonifera Vuillemin. Toney, Trichoderma viride Pers. During the present investigation, Pseudocercospora libenbergii is a new record for Bangladesh.

Keywords: Mycoflora; Symptomatic leaves; Rauvolfia serpentina; Bangladesh.

Location: Botanical garden, Curzon Hall Campus, Dhaka University; Lawachara, Sylhet; Botanic garden, Chittagong University campus and Mymensingh of Bangladesh.

Start Date: 00-00-2007

End Date: 00-00-2013

Program Area: Conservation and Biodiversity

Commodity: Plant

Objectives:

The main purpose of the present study was conducted to find out the existence and identification of mycoflora living on infected R. serpentina phylloplane.

Methodology:

For the present investigation sampling area were field plots of Botanical garden, Curzon Hall Campus, Dhaka University; Lawachara, Sylhet; Botanic garden, Chittagong University campus and Mymensingh of Bangladesh. Two types of symptoms viz., anthracnose and leaf spot were recorded from leaves of R. serpentina during the tenure of 2007 to 2013. Infected leaf samples were placed in clean brown paper bag, labeled properly and preserved at 4ºC in refrigerator for future studies. The fungi associated with the collected samples were isolated following ‘Tissue planting’ method on PDA (Potato Dextrose Agar) medium and ‘Blotter method’. Isolation procedure was followed by (Yasmin and Shamsi, 2015). Detailed morphological studies of the fungal isolates were made in order to determine their identification. The microscopic structural characters of the isolated fungi were recorded under Nikon D 5000 digital camera. Then isolates fungi were identified based on following standard literatures (Barnett and Hunter, 1972; Booth 1971; Ellis 1971, 1976; Ellis and Ellis 1997; Thom and Raper 1945; Raper et al. 1949 and Sutton 1980). The experiment was conducted in the Laboratory of Mycology and Plant Pathology, Department of Botany, University of Dhaka and all the specimens were preserved in this laboratory.

Source of Information: Bangladesh J. Plant Taxon. 27(1): 129?136, 2020 (June)

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

A detailed survey of literature revealed that Pseudocercospora libenbergii has not been reported in any relevant literature of Bangladesh (Talukar 1974, Siddiqui et al., 2007; Shamsi 2017 and 2018). Hence, Pseudocercospora libenbergii Deighton reported here as a new fungal record for Bangladesh.

Knowledge Management: Journal