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Responses of Naphthalene Acetic Acid and Pseudomonas Inoculum on Growth, Yield and Some Biochemical Parameters of Sesame (sesamum Indicum L. Var. BARI Til-4)

Shawline Ema
Department of Botany, Jagannath University, Dhaka-1100, Bangladesh

AMM Golam Adam
Department of Botany, Jagannath University, Dhaka-1100, Bangladesh

Farhana Islam Khan
Department of Botany, Jagannath University, Dhaka-1100, Bangladesh

Hasna Hena Begum
Department of Botany, Jagannath University, Dhaka-1100, Bangladesh

Abstract/ Executive Summary:

An experiment was conducted to evaluate the responses of various concentrations of NAA (25, 50, 75 ppm) and Pseudomonas inoculum on growth, yield and some biochemical parameters of sesame (Sesamum indicum L. var. BARI Til-4). Results revealed that application of NAA treatments and Pseudomonas inoculum reduced plant height non-significantly. Significant result was recorded on number of branches per plants from 50 ppm NAA treated plants. Plants treated with 75 ppm NAA produced maximum number of leaves, fresh and dry weight of leaves and roots, leaf area per plant and specific leaf weight whereas significant variation was found on leaf area. Seeds treated with Pseudomonas inoculum exhibited maximum shoot and root ratio which showed non-significant variation with the results of NAA treated plants. Yield contributing parameters viz., number of pods per plant, fresh and dry weight of pods per plant, 1000-seed weight were recorded maximum from 75 ppm NAA which were statistically similar to control. Maximum length of pod and number of seeds per pod were also recorded from 75 ppm NAA treated plants which eventually produced 32.54% higher yield than control. Harvest index was significantly influenced by all the treatments. Foliar NAA treatments and Pseudomonas inoculum had stimulatory effects on pigment contents of fresh leaves at vegetative stage where, Pseudomonas inoculum treated plants produced significantly higher chlorophyll a and carotenoids content than control. Significantly higher protein content of fresh leaves and seeds were also recorded from 75 ppm NAA treated plants. Out of four treatments, 75 ppm NAA produced better responses in most of the parameters.

Keywords: Sesame, NAA, Pseudomonas, Growth, Yield, Biochemical response

Location: Botanical garden of the Department of Botany, Jagannath University, Dhaka

Start Date: 00-00-2015

End Date: 00-00-2016

Program Area: Crop-Soil-Water Management

Commodity: Sesame

Objectives:

To investigate the effect of foliar application of NAA and Pseudomonas inoculum on growth, yield and biochemical parameters of sesame.

Methodology:

A field experiment was carried out at the Botanical garden of the Department of Botany, Jagannath University, Dhaka during the period of 2015 - 2016. Experimental soil was sandy loam and according to Fertilizer Recommendation Guide (2012) soil contains very low amount of nitrogen (0.069%), very high amount of phosphorus (80.17 μg/g) and very low amount of potassium (0.065 meq/100g). A modern variety of sesame, BARI Til-4 was used as an experimental material. The experiment was laid out in RBD with three replications. Seeds of BARI Til-4 and the strain of Pseudomonas sp. were collected from Bangladesh Agricultural Research Institute (BARI). The total area of the experimental field was 36 m² (6 m × 6 m). The experiment consisted of five treatments viz., T₀ = Control (distilled water), T₁ = Seed inoculated with Pseudomonas sp. T₂ = 25 ppm NAA, T₃ = 50 ppm NAA and T₄= 75 ppm NAA. Seeds were surface-sterilized with 95% ethanol for 5 min and rinsed 6 times with sterile water, then dried overnight in a sterile blotting paper (Atici et al. 2005). Surface sterilized seeds were treated with 1 ml Pseudomonas sp. cell suspension (108 cfu/ml). The seeds of sesame were sown on May 2, 2016 maintaining row to row distance of 30 cm and seed to seed distance of 5 cm. Cultural practices and fertilizer application were done following the Hand Book of Agricultural Technology (Chowdhury and Hassan 2013). Urea as a source of nitrogen was not used in case of bacterial treatment. Foliar application of NAA was done in sunny early morning at the age of 25 days. Growth parameters viz., plant height, number of branches and leaves per plant, fresh and dry weight of leaves and root per plant, shoot-root ratio, leaf area, specific leaf weight and net assimilation rate were recorded at harvest. Pigment and protein contents of fresh leaves were determined at vegetative and flowering stages. Protein contents of fresh seeds was also determined after harvest. The amount of chlorophyll a and b were estimated by using specific absorption co- efficient of Mckinney (1940) and the formulae of Maclachalan and Zalik (1963). The amount of carotenoid was calculated using the equation of von Wettstein (1957). The method of Lowry et al. (1951) was employed for the determination of protein content of leaves. Plants were harvested at the age of 90 days. Nine plants from each treatment were harvested separately to record data on different growth and yield parameters. Data were analyzed statistically, and treatment means were compared by LSD test at 5% level of significance (Steel et al. 1997).

Source of Information: Bangladesh Journal of Botany, 49(2), 335-341; 2020

Article Link (Online Journal): https://doi.org/10.3329/bjb.v49i2.49314

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Results also indicated that 50 ppm NAA had resulted significantly higher amount of seed protein than control but statistically similar to 75 ppm NAA treatment. Protein content of sesame leaves progressively increased with the increasing in concentration of NAA. By applying Pseudomonas aeruginosa LES4,

Knowledge Management: Journal