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Effects of Drought Stress on Growth and Accumulation of Proline in Five Rice Varieties (oryza Sativa L.)

Shukanta Saha
Department of Botany, Jagannath University, Dhaka, Bangladesh

Hasna Hena Begum
Department of Botany, Jagannath University, Dhaka, Bangladesh

Shamima Nasrin
Department of Botany, Jagannath University, Dhaka, Bangladesh

Abstract/ Executive Summary:

A pot experiment was conducted to study the effect of drought stress on growth and accumulation of proline in five rice varieties namely BRRI dhan-30, BRRI dhan-32, BRRI dhan-34, BRRI dhan-38 and BRRI dhan-56 and to characterize them on the basis of their behavior of drought tolerance. Drought stress caused the decrease of growth like root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, shoot dry weight, the ratio of root-shoot length. Among the rice varieties, BRRI dhan-56 showed the lowest decrease of growth of plant. BRRI dhan-56 showed the least decrease of water content in both root and shoot. On the other hand, the accumulation of proline was increased in five rice varieties under stress. BRRI dhan-56 showed the highest (3.7- folds) increase in the accumulation of proline in leaf under stress. This study suggests that BRRI dhan-56 may possess drought tolerance characteristics while BRRI dhan-30, BRRI dhan-32, BRRI dhan-34 and BRRI dhan-38 may be drought sensitive based on their growth and proline accumulation behavior.

Keywords: Drought stress, Growth, Water content, Proline accumulation, Rice

Location: Department of Botany, Jagannath University, Dhaka.

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Rice, Water management

Objectives:

1. To study the effects of drought stress on growth and proline accumulation of five rice varieties; and

2. To characterize their behavior on the basis of drought tolerance.

Methodology:

A pot experiment was carried out at the research garden of the Department of Botany, Jagannath University, Dhaka. Seeds of five rice varieties viz., BRRI dhan-30, BRRI dhan-32, BRRI dhan-34, BRRI dhan-38 and BRRI dhan-56 had been collected from Bangladesh Rice Research Institute (BRRI), Gazipur. Half loamy soil and half compost soil were used by mixing. The pot experiment was set up in net house of the research garden. The seeds were surface sterilized by agitation in 95% ethanol for 1 min followed by five washings with sterile water. Seeds were rinsed in distilled water for about 30 minutes. These seeds were allowed to germinate on a filter paper in Petri dishes, moistened with 4 ml of distilled water. The Petri dishes were arranged randomly and stored at room temperature (24 ± 2°C) under dark conditions. The Petri dishes were covered to prevent the loss of moisture by evaporation under laboratory condition. Within three days the seeds germinated from the date of sowing. Drought stress was imposed by withholding irrigation at 21 days after sowing in 25 pots. Water level in well-watered treatment (control) was maintained at 5 cm above the surface of the soil in the rest 25 pots. The experiment was arranged with CRD with five replications. Plants were collected at 10 days after stress. After taking fresh weight the root and shoot were dried in an oven for 72 hours at 80ºC to a constant weight. Several growth parameters like root and shoot length, length of root/shoot ratio, fresh weight and dry weight of shoot and root were measured. Water contents of root and shoot were determined. Proline accumulation of leaves for each treatment was determined at 16 days after stress following the method of Bates et al. (1973).

Source of Information: Asiat. Soc. Bangladesh, Sci. 45(2): 241-247, December 2019

Article Link (Online Journal): DOI: https://doi.org/10.3329/jasbs.v45i2.46597

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

This study suggests that BRRI dhan-56 variety may possess drought tolerance characteristics while BRRI dhan-30, BRRI dhan-32, BRRI dhan-34 and BRRI dhan-38 varieties may be drought sensitive on the basis of their growth and proline accumulation.

Knowledge Management: Journal