Md. Ahsan Shahriar Tohfa
Department of Zoology, Brahmonbaria Govt. Mohila College, Brahmonbaria, Bangladesh
Tahmina Akter
Department of Zoology, Jahangirnagar University, Savar, Dhaka, Bangladesh
Md. Junayed
Forest Protection Division, Bangladesh Forest Research Institute, Chattogram-4211, Bangladesh
Saadia Ahmad
Department of Zoology, Jahangirnagar University, Savar, Dhaka, Bangladesh
Aedes aegypti, Dengue vector, Fresh seed, Mosquito.
Medical Entomology Laboratory, Department of Zoology Jahangirnagar University, Savar, Dhaka.
Pest Management
Extract (plant, seed)
This experiment was conducted to evaluate larvicidal activity of aqueous fresh seed extracts of 6 indigenous plants against late 3rd instar larvae of Dengue vector mosquito Aedes aegypti (L.) under laboratory conditions (air temperature 270-35°C, water temperature 260-34°C and relative humidity 68%- 88%) from June 2009 to May 2010 in the Medical Entomology Laboratory, Department of Zoology Jahangirnagar University, Savar, Dhaka. To conduct this research work required equipments and other materials were-rearing cage, sweeping net with iron frame, ovitrap, earthen bowl, petri dish, dropper, brush, mosquito net, plastic cup, pipette, cotton, glucose tube, tap water, test plants, Cerelac Baby Food, yeast powder, glucose, pigeon (for blood feeding the adult female mosquitoes), glass beaker, dropper, measuring cylinder, petri dish, thermometer, hygrometer, magnifying glass, plastic cup, funnel, filter paper, conical flask, mortar-pestle etc. For the rearing of Aedes aegypti wild eggs were collected by placing ovitrap in different areas of Jahangirnagar University campus. Ovitraps were made by inserting a long strip of filter paper wrapped inside a black colored glass jar. A little amount of water was kept in the bottom of glass jar so that some portion of the filter paper became wetted and moistened. Aedes mosquito laid eggs on the moist surface of the filter paper. After collecting the eggs, the egg strips were dried in the air for 1-2 days. Then the egg strip was placed in normal tap water for hatching. The hatched larvae were reared in normal laboratory condition. They were kept in an earthen jar and provided Cerelac baby food and yeast granules as larval food daily. In order to prevent egg laying by other mosquito species the bowl were kept in mosquito rearing cage. The plant seeds were collected from Jahangirnagar University campus and surrounding areas. Fresh seeds were crushed and powdered with the help of mortar pestle, grinder and blender. Then the powdered seeds were weighted with the help of electric balance for different concentrations (5%, 4%, 3%, 2%, 1%). Fresh seed extract was prepared by mixing the powdered seeds (weighed 100gm) in a beaker filled with 1900ml of distilled water. Then the total amount of solution was 2000 ml and it was left for 24 hours for extraction to settle down. After a successful extraction the solution was filtered by filter paper. This is the stock solution (concentration 5%). The dose response data were analyzed by using Probit Analysis Program Version 1.5 developed by the ‘Ecological Monitoring Research Division’, Environmental Monitoring Systems Laboratory, U. S. Environmental Protection Agency (EPA), Cincinnati, Ohio 45268. The program is used to determine LC50, LC90 and LC95 values. LC values were determined to compare the larvicidal effects of various seed extract. For multiple group comparisons, differences of means among groups were compared using one way analysis of variance (ANOVA). DMRT (Duncan Multiple Range Test) was done using SPSS (Statistical Package for Social Science) program (version 12). Graphical representations were done using Microsoft Office Excel 2007.
International Journal of Biosciences; ISSN: 2220-6655 (Print), 2222-5234 (Online); Vol. 17, No. 4, p. 46-59, 2020
Journal