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A Comparative Analysis on Mitotic Interphase and Prophase Among Twelve Varieties of Brassica L. from Bangladesh: Brassicaceae

Susmita Saha
Department of Botany, Faculty of Life and Earth Sciences, Jagannath University, Dhaka, Bangladesh

Kazi Nahida Begum
Department of Botany, Faculty of Life and Earth Sciences, Jagannath University, Dhaka, Bangladesh

Abstract/ Executive Summary:

Brassica L. is an agronomical and economical important crop belonging to Brassicaceae family. The research was conducted to interrelate interphase nuclei and prophase chromosome of Brassica L. varieties. In the current analysis, the nature of interphase nuclei and prophase chromosome of twelve BARI varieties of Brassica L. was investigated based on orcein-staining properties. In the interphase nuclei only ‘Diffuse type’ and ‘Simple Chromocenter type’ were found in Tori-7, Dawlat, BS-11, BS-14 and SS-75, Rai-5, BS-6, BS-7, BS-8, BS-10, BS-12, BS-15, respectively. Considering prophase chromosome among these studied twelve varieties, ‘Continuous type’ showed in Dawlat, Rai-5, BS-7, Bs-8, BS-11, BS-14 while BS-6, BS-10, BS-12 and BS-15 observed ‘Interstitial type’ and ‘Gradient type’ showed in Tori-7, and SS-75. Therefore, the orcein staining property of interphase nuclei and prophase chromosome can provide essential information as cytological implement to discriminate the twelve analyzed Brassica L. varieties.

Keywords: Brassica., Interphase, Prophase, Orcein-staining.

Location: Oil Research Center (ORC) of Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Mustard

Objectives:

To interrelate interphase nuclei and prophase chromosome of Brassica L. varieties.

Methodology:

Plant materials- To conduct this current investigation, all the twelve varieties of Brassica L. were assembled from Oil Research Center (ORC) of Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur and then maintained in the Botanical garden of Jagannath University, Dhaka, Bangladesh. Sample preparation- At first the wet filter papers were placed in a Petri plate to germinate seeds for 36 h and 15 min at room temperature in the dark and Bulb tips (RTs) were gently taken from germinated seeds. Then, by using 8-hydroxyquinoline (0.002 M) for the RTs were pretreated 50 min at room temperature and fixed in Carnoy’s fluid (Glacial acetic acid Ethanol 1: 3) at 4°C for 24 hours and then preserved in 70% alcohol for later use. Next, a mixture of 1% aceto-orcein and 1 N HCl was used to heat the pre-treated RTs through a spirit burner. Afterwards, the prepared aceto-orcein stained RTs were mildly squashed and finally prepared slides were examined under the Optika electric microscope and microscopic images were captured by the Euromax camera (CMEX 10, DC 10000C) with 100X magnification in Auto mode. Analytic form- For considering the behavior of interphase and prophase of inspected twelve Brassica L. varieties, from 40 to 50 interphase nuclei and prophase chromosome were examined and then categorized on the basis of Tanaka's (1971) classification.

Source of Information: International Journal of Biosciences; Vol. 17, No. 4, p. 73-82, 2020

Article Link (Online Journal): http://dx.doi.org/10.12692/ijb/17.4.73-82

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It may be concluded that the present investigation correlated the nature of mitotic interphase nuclei and prophase chromosome of twelve BARI varieties of Brassica L. Moreover, this investigation might be a contribution to count the nature of mitotic interphase nuclei and prophase chromosome as a cytological tool and provided sufficient information to categorize as well as further investigation of investigated twelve varieties of Brassica L. from Bangladesh.

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