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Genetic Mapping for Quantitative Trait Loci for Maturity of Mungbean Vigna Radiata L. Wilczek.

A.K.M. Mahbubul Alam
Pulses Research Centre, BARI, Ishurdi, Pubna, Bangladesh

Abstract/ Executive Summary:

Mungbean (Vigna radiate (L) Wilczek), varieties with synchronous maturity is generally preferred for cultivation. In this study, we report quantitative trait locus (QTL) mapping in mungbean using an F₂ population of 142 lines derived from a cross between KPS-I (75-80 days) and BARI mung 6 (55-60 days). F_2:3 lines grown under field condition at Gazipur 2016. Single marker analysis suggested at least five loci controlling maturity. Composite interval mapping consistently identified four QTLs, qMAT1, qMAT2, qMAT3, qMAT6 and qMAT8, on linkage groups 1, 2, 3,6 and 8. These QTLs accounted for 7.54 to 18.83% variation depending on different linkage group.

Keywords: Pulse, Mungbean, Lentil, BARI mung 6, Grain yield

Location: PRSS, Gazipur, Bangladesh

Start Date: 00-00-2015

End Date: 00-00-2016

Program Area: Crop-Soil-Water Management

Commodity: Mungbean

Objectives:

To determine the Genetic Mapping of quantitative trait loci for Maturity of Mungbean

Methodology:

The F_2:3 progenies and parents were evaluated for synchrous maturity at of PRSS, Gazipur 2016. 142 F_2:3 lines were sown together with their parents in randomized complete block design (RCBD) with two replications. Each entry was sown in two rows with 2 m long. Row to row distance was 40 cm with spacing between adjacent plants of 10 cm. Fertilizers were applied at 40kg/ha urea, 90kg/ha triple super phosphate, 60 kg/ha muriate of potash and 40kg/ha gypsum prior to soil preparation following recommendation guide of BARI for mungbean. Weeds were controlled manually. Harvesting was done by hand. The average data for maturity in each entry were used for statistical and QTL analysis in each of the experiments. Analysis of variance (ANOVA) was carried out for the disease score data from individual experiment and combined data using the WinQTL Cartpgrapher and JoinMap 4.0 (Van Ooijen 2006) software. The linkage groups were calculated with the minimum logarithm of the odds (LOD score) at 3.0, the maximum recombination frequency (r) at 0.5. and Kosambi mapping function (Kosambi 1944). Marker scores were subjected to goodness of fit test using chi-square analysis (1:2:1 and 1:1 ratios in the F_2:3 populations.

Source of Information: Annual Research Report-2015-2016, Pulses Research Centre, BARI, Ishurdi, Pubna, Bangladesh

Article Link (Online Journal):

Funding Source:

1.

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The F_2:3 progenies and parents were evaluated for synchrous maturity at of PRSS, Gazipur2016. 142 F2:3 lines were sown together with their parents in randomized complete block design (RCBD) with two replications. Each entry was sown in two rows with 2 m long. Row to row distance was 40 cm with spacing between adjacent plants of 10 cm. Fertilizers were applied at 40kg/ha urea, 90kg/ha triple super phosphate, 60 kg/ha muriate of potash and 40kg/ha gypsum prior to soil preparation following recommendation guide of BARI for mungbean. Weeds were controlled manually. Harvesting was done by hand. The average data for maturity in each entry were used for statistical and QTL analysis in each of the experiments.

Knowledge Management: Report/Proceedings