Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Development of Callus Initiation and Regeneration System of Different Indigenous Indica Rice Varieties

Kazi Muhammad Ahasanul Hoque*
Depart. of Genetic Engineering and Biotechnology, Shahjalal University of Sci. and Tech., Sylhet 3114, Bangladesh

Z.A. Azdi
Depart. of Genetic Engineering and Biotechnology, Shahjalal University of Sci. and Tech., Sylhet 3114, Bangladesh

Shamsul H. Prodhan
Depart. of Genetic Engineering and Biotechnology, Shahjalal University of Sci. and Tech., Sylhet 3114, Bangladesh

Abstract/ Executive Summary:

The aim of the present study was to develop an efficient protocol for best callus induction and complete plant regeneration for varieties of rice (Oryza sativa) i.e. Pakhi Biroin, Hati Baromashi, Kacha Biroin, Badal Boro, Porichok Amon, Khoiya Boro, Joria Aman and BRRI Dhan53. Pakhi Biroin, HatiBaromashi, BadalBoro, Porichok Amon, KhoiyaBoro and Joria Aman cultivate mainly in Sylhet & Sunamgonj district of Bangladesh. For callus induction from mature dehusked rice seeds MS (Murashige & Skoog, 1962) media supplemented with different concentrations i.e. (1.5, 2.0, 2.5, 3.0 mg/L of 2,4-D (2,4 dichlorophenoxyacetic acid)) were used. Khoiya Boro and Joria Aman did not show any efficiency in callus induction. Maximum percentage of callus induction efficiency that is 100% was noted for Pakhi Biroin, Hati Baromashi and BRRI Dhan53, but 75% was the highest percentage of callus for Kacha Biroin. For plantlet regeneration of Pakhi Biroin, Hati Baromashi and Kacha Biroin, MS media with 0.5 mg/L NAA (1-Napthaleneacetic acid), 0.5 mg/L Kinetin and various concentrations of BA (6-benzyladenine) (1.0, 2.0, 3.0, 4.0 mg/L) were employed. On the other hand, MS media with 1.0 mg/L NAA, 1.0 mg/L Kinetin and various concentrations of BA (1.0, 2.0, 3.0, 4.0 mg/L) were used for BRRI Dhan53 regeneration. Different result was recorded for different varieties at various hormone concentrations.

Keywords: Mature Embryos, Genotypic Variability, Embryogenic Callus, Plant Regeneration, Indica Rice

Location: Department of Genetic Engineering and Biotechnology, Shahjalal University of Science & Technology (SUST), Sylhet, Bangladesh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Rice

Objectives:

Objective of the present research was to study the potentiality of the varieties in tissue culture as well as to determine the most suitable concentration and combination of growth regulators for excellent callus induction and regeneration which is of great impotence for gene transformation to create high yielding varieties.

Methodology:

This research work was conducted at the plant genetic engineering lab of Department of Genetic Engineering and Biotechnology, Shahjalal University of Science & Technology (SUST), Sylhet, Bangladesh. Mature Dehusked rice seeds were taken as source of explant. Dehusked seeds were first washed in distilled water mixed with Tween 20 (one drop/30 ml of water) for ten minutes, steeped in 70% (v/v) ethanol for two minutes with gentle agitation followed by rinsing three times with sterile distilled water. The seeds were surface sterilized in 0.1% (w/v) HgCl2 for 15 minutes with gentle agitation and rinsed five times with sterile distilled water. Surface sterilized seeds were thoroughly washed six to ten times with autoclaved sterile distilled water. The seeds were finally placed on the sterilized petriplate having sterile filter papers with the help of forceps to remove excess water. After removing the water from the seeds surface, these seeds were inoculated into culture tubes containing MS (Murashige & Skoog, 1962) basal media supplemented with different concentrations of 2, 4-dichlorophenoxyac-etic acid (2,4-D) culture tubes were transferred and maintained in an environmentally controlled growth room for 4 weeks for callus and subcultured in the same medium for 3 weeks. Regeneration efficacy was observed with MS media supplemented with different combination and concentration of NAA, Kn (Kinetin) and BA. The pH of the media was adjusted to 5-8 with acid and alkali. The media was autoclaved at 15 pound square inch (psi) for 20 minutes at 121°C. Inoculation was carried out under a sterilized environment in a laminar air flow cabinet. All cultures were incubated at 25±1 °C with a photoperiod of 12 hours at 2000 lux light intensity of cool white fluorescent light. The response of all varieties of rice was determined in terms of callus induction, callus growth and regeneration frequencies. The data of callus growth was subjected to ANOVA (Analysis of Variance) testing and Standard error (SE) for 5% of means was calculated by using standard statistical MSTAT-C software.

Source of Information: Journal of Biology (2013), Vol. 01, Issue 02, pp. 46-51 ISSN 2050-0751

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The investigation was conducted for establishment of callus initiation and regeneration system for these local rice varieties using mature seed embryo as explants. This highly efficient, reproducible system will be used to develop genetic transformation techniques for these important rice cultivars.

Knowledge Management: Journal