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Development of Culture Technique for Live Feed Isolation from the Bay of Bengal

Jakia Hasan
Senior Scientific Officer
Marine Fisheries & Technology Station, BFRI, Cox's Bazar

Abstract/ Executive Summary:

From Bay of Bengal 6 genus of Dinophyceae, 3 genus of Bacillariophyceae, 6 genus of Coscinodiscophyceae, 3 genus of Cyanophyceae, 2 genus ofFragilariophyceae, 2 genus of Mediophyceae and 1 genus of Eustigmatophyceae (total 23 genus) were identified. For the isolation of rotifer (Brachionus rotundiformes) firstly, 5ml plankton sample (250µm zooplankton net) were collected from Bakkhali River at Nuniarchara, in Cox’s Bazar. A micro pipette were used to separate individual cells from crude plankton sample through a series of sterile washes under a microscope. Single cells were transferred from the first drop to the next drop until only one single cell was present in a drop of sample. Finally, the single-cell was transferred to a glass jar containing Nanno chloropsis culture water. This microalgae and rotifer mixed culture water kept in a chamber of live feed lab was no light and maintaining 25 ppt and 24 hours aeration.

Keywords: Live Food, Algae, Bay of Bengal

Location: Bay of Bengal, Bangladesh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Algae

Objectives:

To identify the commercially important microalgae from the Bay of Bengal
To scale up the identified microalgae in vitro condition
To investigate the nutritional status of identified microalgae
To use identified microalgae as feed additives in fish larvae feeds

Methodology:

Firstly collected plankton using 20 µm mesh size netting material in open sea and Bakhkhali River and kept it in sampling bottles. Then the sample was transported sampling bottles in Styrofoam box with ice to maintain 20-25⁰C temperature. Examined the sample under microscope and found our desirable some species based on pigmentation, morphology (coccoid, filamentous), size, the presence or absence of flagella and other distinctive features (Kumar 1990; John 2012a,b) using an electric light microscope (Leica DM1000 Microscope made by Switzerland)) at 10x, 20xand 40x. Various types of microalgae were observed. Then the sample was enriched with Gillard and Ryther’s Modified f/₂ medium and cultured it with suitable lab condition (Aeration and Light). For the pure culture development of Nannochloropsis sp. in 50 ml firstly10 ml sample of the serial dilution test tube (10^-14) mixed with 40 ml autoclaved seawater in sterilized 50 ml conical flask. Then added 100 µl f media part without sodium silicate. The culture was kept in light with a photoperiod of 24 hours and gently shaken twice in daily at morning and afternoon in a fixed time. This process was carried on up 8days and cell density was observed after every day with drawing a sterilized micropipette through a microscope.

Source of Information: Annual Report 2017-19. 232 p.

Article Link (Online Journal):

Funding Source:

1. Government

Budget: 700,000

Total Budget (Tk.) : NaN

Achievement/Findings:

Six genus of Dinophyceae, 3 genus of Bacillariophyceae, 6 genus of Coscinodiscophyceae, 3 genus of Cyanophyceae, 2 genus ofFragilariophyceae, 2 genus of Mediophyceae and 1 genus of Eustigmatophyceae (total 23 genus) were identified. Two phytoplankton species (Skeletonema sp., Nannochloropsis sp.) and one Zooplankton (Brachionus rotundiformes) were isolated from the Bay of Bangle. This Phytoplankton species showed maximum growth in 28 ppt than that of other salinity.

Knowledge Management: Report/Proceedings