Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Brood Development and Seed Production of Commercially Important Marine Fishes

Dr. Ehsanul Karim
Senior Scientific Officer
Bangladesh Fisheries Research Institute

Abstract/ Executive Summary:

Artificial breeding trial of Mugil cephalus L. using hormones was conducted from August, 2014 to February, 2015 in a fish hatchery, Cox’s Bazar, Bangladesh. In this experimental trial, average one kg sized M. cephalus were collected from wild and reared in saline-water ponds to breed in captivity through hormone induction. The salinity of rearing ponds was maintained between 20 – 25ppt and in hatchery 24 – 25ppt with 22 – 25ºC temperature. Carp pituitary glands (CPG), HCG (Human chorionic gonadotropin) and LRH-A2 (Luteinizing releasing hormone) were used for hypohydration. The effective dosages of CPG 30mg/kg body weight, LRH-A2 150μg/fish with a combination of 0.3 mL Domperidone and 0.5 mL Calcium injection, and HCG dose of 30,000 IU in case of female and 5,000 IU in case of male resulted spawning success. The GSI value of fecund fishes ranged from 7.92 to 12.38, egg diameter of matured fish between 550 to 600μm and that of a fertilized egg from 650 to 700μm. Fecundity was calculated as 735 to 900 nos/g. The fish started spawning between 44 – 48 h and cell division was observed after the first hour of spawning but severe mortality occurred after 6 h.

Keywords: Mullet, Mugil cephalus, Breeding

Location: Cox’s Bazar, Bangladesh

Start Date: 00-08-2014

End Date: 00-02-2015

Program Area: Variety and Species

Commodity: Marine fish

Objectives:

To develop broods through rearing of wild fish and reproductive biology study of mullet (Mugil cephalus)
To develop induced breeding techniques of mullets using different hormonal doses.
To refine live feed culture technique in hatchery for larval rearing of mullet.

Methodology:

The experiment was conducted from August, 2014 to February, 2015 in a fish hatchery at Rejukhal under Cox’s Bazar district, Bangladesh. Adult/sub-adults of mullets, M. cephalus were collected from the wild and stocked in the ponds of Niribili Fish Farm, Rejukhal in the month of August, 2014. The fish were fed with commercial floating feed (Mega Feed) twice daily at 2 – 2.5 per cent of their body weight and Vitamin-E (Selvitdex) was added to feed during the month of October to November for gonadal maturation. After transportation of broods, they were treated with Furacin (50ppm) to avoid possible infection, females and males were separated and oocytes were sampled following LOB (Live Ovarian Biopsy) method. In this method, catheter (no. 6) gently pushed into the gonad then sucked by mouth for collecting gonad and collected sample observed under microscope to check the development of gonads. Hormone injections were initiated within 48 h after transportation and acclimatization for 24 h. Interval between injections varied from 24 to 36 h. Dry carp pituitaries (CPG) for 1st dose and LRH-A2 (Luteinizing releasing hormone) for 2nd dose with the combinations of Domperidone and Calcium injections were injected in varied doses. HCG was also used for the 1st dose of male in the first trial and both male/female in the second trial. In case of both female and male, hormone was injected in deep muscle at base of the dorsal fin. Natural spawning spawning with two un-injected males in holding tanks was also performed. Spawning behavior in holding tank was closely monitored. The released eggs were floating and drifting in nature. In case of fecundity study, the spent females were dissected and eggs retaining in the abdomen were counted volumetrically for the measurement of fecundity. Released eggs were continuously monitored by using ocular and stage micrometer to estimate eggs diameter. Fertilized eggs were kept in the spawning tank for incubation after treated with streptomycin solution at 0.5ml/L and observation continued through microscope until the starting of cell division.

Source of Information: Annual Report 2017-19. 232 p.& Bangladesh J. Zoology, 43(2): 279-288.

Article Link (Online Journal):

Funding Source:

1.

Budget:

Total Budget (Tk.) :

Achievement/Findings:

This was the first attempt of establishing induced breeding protocol of Mullet fish in coastal areas of Bangladesh. It was reavled that induced breeding of M. cephalus is possible in our environment and also it could be successfully hypophyzed with exact hormonal doses. Further study should be conducted using quality broods for fine tuning of hormone induced artificial breeding. After transportation of broods, they were treatmented with Furacin (50ppm) and females and males were separated and oocytes were sampled by following Live Ovarian Biopsy (LOB) method. Injections were initiated within 48 hrs after transportation and acclimatization. Interval between injections varied from 24 to 36 hrs. Dry carp pituitaries (FAO) for 1^st dose and LRH A₂ (2^nd dose) with the combinations of Domperidone and Calcium injections were injected in varied total dose and also used HCG for the 1^st dose of male and both male/female in the second trial. In case of both female and male, hormone was injected in deep muscle at base of the dorsal fin. Spawning behavior was closely observed visually. Eight pairs kept in eight tanks but not found any positive response. In case of fecundity study, the females were dissected and eggs retaining in the abdomen were counted for the measurement of fecundity volumetrically. Eggs were continuously monitored by using ocular and stage micrometer to estimate eggs diameter and also checked oil globules diameter.

Knowledge Management: Journal