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Surveillance and Distribution of Pathogenic Agents (virus/bacteria) of Shrimp/prawn Disease in Bangladesh

H. M. Rakibul Islam
Senior Scientific Officer
Shrimp Research Station, Bangladesh Fisheries Research Institute

Abstract/ Executive Summary:

Shrimp gher and hatcheries including SRS golda hatchery, BRAC hatchery and Kakchira hatchery were investigated for mass larval mortality issue. Bacterial colony count and MrNV viral test using PCR were carried out. Vibrio load was under the allowable limit, however, bacterial load for enteric Bacilli was 1.34 x 10⁴, 7.84x10⁴ and 1.78 x 10⁵ cfu for SRS golda hatchery, BRAC hatchery and Kakchira hatchery respectively. PCR showed few inspecific bands resembling MrNV positive. WSSV, AHPND, NHP and EHP. Samples were tested using self-developed gene specific primer covering 1^st step and Nested PCR protocol.

Keywords: Shrimp, Diseases, PCR

Location: Bangladesh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Shrimp

Objectives:

To Investigate the emerging diseases of shrimp and prawn
To identify the pathogenic agent of NHP, EMS /AHPND and EHP using PCR
To identify the Risk-factors of the disease invasion
To identify the available strains of White Spot Syndrome Virus (WSSV) causing shrimp (P. monodon) mortality

Methodology:

From the sampling area samples were collected for PCR identification. From every sample, one set of Hepatopancreas (HP) was crushed and incubate over night at TCBS Agar Media. After overnight culture, greenish colonies collected and make pellet. Then these pellets were stored use for PCR identification by using IQ2000 toxin kit suggested method. Along with the bacterial pellets, raw HP and gill sample were used for PCR identification for EMS and WSSV detection. Identification EHP was carried out using the PCR test kit.

Source of Information: Annual Report 2017-19. 232 p.

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) : 23,00,000

Achievement/Findings:

About 50 shrimp ghers and 9 hatcheries of Bagerhat, Khulna, Satkhira district were investigated randomly in context to aqua ecology and pathogens. From the sampling area samples were collected for PCR identification. Among those, 67% of them are EMS and WSSV positive where 32% found only EMS Positive, 16% found only EMS Toxin Positive, 12% found both EMS and WSSV Positive & 7% WSSV Positive. Among the investigated ghers, 32 were Extensive ghers, 17 were Semi-intensive ghers and 9 were Bahda Hatchery. In Satkhira district, 16 samples found EMS positive out of 30 samples where 19 samples out of 33 were positive from Khulna district. In case of Bagerhat district, 4 samples were EMS positive out of 28 samples

Knowledge Management: Report/Proceedings