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Research Detail

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Soma Dey
Institute of Forestry and Environmental Sciences, University of Chittagong, Chattogram, Bangladesh

Mohammed Kamal Hossain
Institute of Forestry and Environmental Sciences, University of Chittagong, Chattogram, Bangladesh

Md. Danesh Miah
Institute of Forestry and Environmental Sciences, University of Chittagong, Chattogram, Bangladesh

The study was investigated to inspect the effect of different pre-sowing treatments on germination and initial growth performance of Ehretia serrata seeds at the nursery of Chittagong University, Bangladesh. Seeds were treated to six pre-sowing treatments e.g. Control (T0), seeds immersed in hot water for 1 min (T1), seeds treated with sandpaper rubbing (T2), immersed in hot water for 30 sec (T3), immersed in cold water 24 hours (T4), immersed in H2SO4 for 30 sec (10% solution) (T5). Forest topsoil and fine Sylhet sand were used as growing media. As seeds were very tiny in size, germination trays were used to make the experiment convenient. Germination percentage (78.43 %), germination uniformity (0.0235), and germination value (4.8630) were recorded highest in T5 treatment (immersed in 10% H2SO4 and significantly (P< 0.05) different from other treatments. Maximum shoot height (11.34 cm) and root height (9.96 cm) were revealed in T0 treatment. Finally, seeds immersed in H2SO4 for 30 sec (10% solution) were found suitable for maximum seedlings production. In the case of vigor seedling production, seedlings raised in forest soil were revealed suitable for Ehretia serrata.

  Pre-sowing treatments, Germination, Seedling growth, Germination tray, Growing media, Germination value
  Forestry and Environmental Sciences, University of Chittagong, Chattogram, Bangladesh
  00-10-2018
  00-03-2019
  Development of Host and Medicinal Plants
  Medicinal Plants

To study the effect of pre-sowing treatments on seed germination to identify appropriate pre-sowing treatment for Ehretia serrata.

Study site- The present study was carried out during October 2018 to March 2019 in the nursery of Institute of Forestry and Environmental Sciences, University of Chittagong, Chattogram, Bangladesh (lies between 91°50´E longitude and 22°30´N latitude). The climate is tropical monsoon with an average monthly highest temperature of 29.75°C and a monthly lowest of 21.24 °C. The maximum temperature usually occurs in May as 32.60 °C and the minimum in January as 14.10 °C (Peel et al., 2007). Seed collection- E. serrata fruits were collected from Bohaddarhat, Bangladesh during October 2018. Phenotypic characteristics of seeds were measured. Fruits were immersed in water for overnight and extracted seeds, dried in the open sun for three days. Randomly selected seeds length and width are respectively 3.22±0.07 mm and 0.0056±0.002 mm. About 178,000 seeds were found in one kg. Experimental design As the seeds were tiny and hard to make the experiment convenient, seeds were sown in germination tray. The media of the germination tray was forest topsoil collected from the forest floor and fine Sylhet sand. The investigation is made up of six pre-sowing treatments with 3 replications (15 seed per replication) in a randomized complete block design. Forty-five (45) healthy seeds were chosen randomly from each treatment. Daily germination was recorded as soon as the seeds start germination. Seedlings raised in the germination tray were transferred to polybag after 2 months of the last germination of seeds. The pricked out seedlings were kept in shade for 2 weeks and then transferred to sunlight. Proper care and maintenance were done regularly. Six seedlings from each treatment were selected randomly and shoot height and root height of the seedlings were recorded. The pre-sowing treatments are : T0- Seeds without treatment and sown in germination tray (soil media), Control; T1- Seeds immersed in hot water for 1 min and sown in germination tray (fine sand media); T2-Seeds treated with sandpaper rubbing and sown in germination tray (fine sand media); T3- Seeds immersed in hot water for 30 sec and sown in germination tray (fine sand media); T4- Seeds immersed in cold water for 24 hours and sown in germination tray (fine sand media); T5- Seeds immersed in H2SO4 for 30 sec (10% solution) and sown in germination tray (fine sand media). Germination capacity: It is the percentage of seeds germinated in an experiment from the starting to end. It was classified as follows: a) 90-100%-very good, b)70-90%-good, c)50-70%-average, d) 30- 50%-poor e) 20-30%-very poor, and f)(<) less than 10% extremely poor (Kumar, 1999). Statistical analysis- All the recorded data were analyzed statistically by using computer package software SPSS ver. 23. Duncan’s Multiple Range Test (DMRT) was employed to define the statistical significance and it was shown by different letters in the tables.

  International Journal of Forestry, Ecology and Environment, 02(02), 79-86. 30 August 2020.
  https://doi.org/10.18801/ijfee.020220.09
Funding Source:
1.   Budget:  
  

It can be concluded from the study that seeds immersed in H2SO4 for 30 sec (10% solution) and sown in fine sand media are recommended for the best germination performance of E. serrata. After 2 months of germination, seedlings need to transfer to polybags filled with soil media.

  Journal
  


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