M Moniruzzaman
Horticulture Research Centre, BARI, Joydebpur, Gazipur Bangladesh
N. Ara
Horticulture Research Centre, BARI, Joydebpur, Gazipur Bangladesh
M. Moniruzzaman
Horticulture Research Centre, BARI, Joydebpur, Gazipur Bangladesh
M.D. Hossain
Horticulture Research Centre, BARI, Joydebpur, Gazipur-1701
M A Goffar
Horticulture Research Centre, BARI, Joydebpur, Gazipur Bangladesh
R. Khatoon
Horticulture Research Centre, BARI, Joydebpur, Gazipur Bangladesh
Drought stress, Growth, Yield, Tomato
Plant Physiology Section, Horticulture Research Center, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh
Variety and Species
Tomato, Drought
The experiment was conducted at the field of Plant Physiology Section, Horticulture Research Center, Bangladesh Agricultural Research Institute (BARI) (23.99307 N, 90.41094 E) during October 2017 to March 2018. Ten varieties of tomato (V1 = BARI Tomato-2, V2 = BARI Tomato-3, V4 = BARI Tomato-8, V5 = BARI Tomato-11, V6 = BARI Tomato-14, V7 = BARI Tomato-15, V8 = BARI Tomato-16, V9 = BARI Tomato-17, V10 = BARI Tomato-18 and V11 = BARI Tomato-19) collected from Olericulture Division of HRC, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur, were evaluated at drought stress-i) non stress- water applied upto 100% of field capacity, ii) drought stress-water applied up to 40% of field capacity. The moisture percent of soil was monitored up to experimentation by Soil Moisture Meter (Model:PMS-714, Taiwan) (Fig. 1). The field capacity of pot soil was 32%. Water was applied to each pot treatment wise. The study was evaluated under Completely Randomized Design (CRD) with three replications. Thirty day-old seedling was transplanted in plastic pot (height- 29 cm, dia 32 cm at the top and 21 cm at the bottom; capacity 14 L) which contained around 11.0 kg soil collected from Kodda, Kaliakoir, Upazilla, Gazipur district. Fertilizers were applied to each pot @ Urea 2.25 g, TSP 1.5 g, MoP 1.70 g, Gypsum 1.5 g and Boric Acid (medicated) 1.0 g during pot filling mixing with air dried soil (12.0 % moisture). Fertilizer was applied 3 times, at the rate of 4.0 -2.0-2.0 g of Urea, TSP and MoP per pot on 14 December 2017, 08 January 2018 and 04 December 2018 at 15 days interval. Weeding was done as per requirements. Ridomyl Gold was sprayed @ 0.2% two times when symptom of late blight tomato was seen. Zinc deficiency symptom was seen and zinc sulphate was sprayed @ 5.0 g/L H2O on 3rd January 2018. Two times rainfall occurred during experimentation on 09 December 2017 and 26 February 2018. Final harvest of tomato was done 26 March, 2018. Data (from 5 plants) were taken on plant height, chlorophyll content (SPAD value), Fv/Fm value, % relative water content, no. of fruits/plant, individual fruit weight, %fruit set and fruityield/pot. The SPAD value was taken by SPAD meter (SPAD-500 plus, Minolta Konica Inc, Japan). The changes in fluorescence yield reflect changes in photochemical efficiency and heat dissipation. The polyphasic rise of fluorescence transients was measured by an ADC Infrared Gas Analysis plant Efficiency Analyzer (PEA, Handsatech Instruments Ltd., King’s Lynn, UK). The initial fluorescence (F0), maximum fluorescence (Fm) were analyzed and quantum efficiency of open photosystem II centres-quantum yield (Fv/ Fm) was calculated. The leaf discs were previously adapted to the dark for 20 minutes. The fluorescence data were collected every day at 10.00 am to 12.00 pm from initial to end of the experiment. Fo is the amount of light absorbed initially to raise the fluorescence from a low level to maximum value Fm after dark adaptation. Fv = Fm-F0 which is the variable. Ratio of Fv/Fm is a dark adapted test used to determine maximum quantum yield. This ratio is also an estimate of the maximum portion of absorbed quanta used in PSII reaction centers. Relative water content (RWC) was measured using leaves (8th no. leaf from the tip of the plant) after imposing drought conditions. Immediately after cutting at the base of lamina, leaves were sealed within plastic bags and quickly transferred to the laboratory. Fresh weights were determined within 2 h after excision. Turgid weights were obtained after soaking leaves in distilled water in plastic bowls for 16 to 18 h at room temperature (about 25°C) and under the low light conditions of the laboratory. During soaking bowls are covered with polythene. After soaking, leaves were quickly and carefully blotted dry with tissue paper in preparation for determining turgid weight. Dry weights were obtained after oven drying the leaf samples for 72 h at 70°C.
Annual Research Report 2017 – 2018, Horticulture Research Centre, BARI, Joydebpur, Gazipur Bangladesh
Report/Proceedings