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Shamim Shamsi
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Sarowar Hosen
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Custard apple (Annona squamosa L.) showing fruit rot symptom was studied. A total of four fungal species were found to be associated with fruit rot of custard apple. The associated fungi were Alternaria alternata (Fr.) Kerissler, Colletotrichum orbiculare (Berk. & Mont.) Arx., Fusarium Link. and Lasidiodiplodia theobromae (Pat.) Griff. & Maubl. Out of four fungal species Lasidiodiplodia theobromae showed the highest (34) frequency whereas Alternaria alternata showed the lowest (10) frequency percentage of association.  

  Custard apple, Tissue planting method, Fruit, Fungal species, Rotted fruits
  Kachukhet Bazar, Dhaka Cantonment, Dhaka, Bangladesh
  00-01-2016
  00-03-2016
  Crop-Soil-Water Management
  Custard apple, Fungal Disease

To find out the association of mycoflora with fruit rot of custard apple. 

Annona squamosa showing fruit rot symptom was collected from the Kachukhet Bazar, Dhaka Cantonment, Dhaka during January to March, 2016. Samples were collected in separate sterile polyethylene bags, labeled properly and then brought to the laboratory. The fungi associated with samples were isolated following “Tissue planting method” on PDA medium. Percentage frequency of the occurrence of the fungal isolate was calculated by adopting the formula of Spurr and Wetly (1972): % frequency = {(Total no. of inocula from which a fungal isolate was observed) / (Total no. of inocula)} x 100. Morphological structures of the fungi were drawn in detail by the aid of Camera Lucida. Identification of the isolates was determined following the standard literatures (Booth 1971, Ellis1971, Barnet and Hunter 1972, Sutton 1980). 

  Bangladesh J. Sci. Res. 29(2): 173-176, 2016 (December)
  
Funding Source:
  

A total of four species of fungi were found to be associated with rotted fruits of custard apple. The isolated fungi were Alternaria alternata (Fr.) Kerissler, Colletotrichum orbiculare (Berk. & Mont.) Arx., Fusarium Link. and Lasidiodiplodia theobromae (Pat.) Griff. & Maubl. (= Botryodiplodia theobromae). 

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