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Phytochemical Investigation and Evaluation of Antioxidant Activities of Methanolic Extract of Globba Marantina Leaves

Shahnaz Naznin
Department of Pharmacy, Noakhali Science and Technology University, Noakhali-3814, Bangladesh

Mahmuda Ferdous
Department of Pharmacy, Noakhali Science and Technology University, Noakhali-3814, Bangladesh

Mohammad Sarowar Uddin
Department of Pharmacy, Noakhali Science and Technology University, Noakhali-3814, Bangladesh

Farjana Yeasmin
Department of Pharmacy, Noakhali Science and Technology University, Noakhali-3814, Bangladesh

AFM Shahid Ud Daula
Department of Pharmacy, Noakhali Science and Technology University, Noakhali-3814, Bangladesh

Mohammad Anwarul Basher
Department of Pharmacy, Noakhali Science and Technology University, Noakhali-3814, Bangladesh

Abstract/ Executive Summary:

Globba marantina is a herbaceous, perennial plant with erect, unbranched stems up to 50cm tall. The plant is harvested from the wild for local use as food and medicine. The plant used to cure earache and as eye, drops to cure conjunctivitis. The small bulbils are used to stimulate the appetite. So, the present study is designed to investigate the possible chemical groups, total phenolic content and antioxidant activities of the crude methanolic extracts of Globba marantina leaves belonging to the family Zingiberaceae. The investigation revealed the presence of alkaloids, cardiac glycosides & steroids. Three complimentary test systems, namely total phenolic content, total flavonoid content and total flavonol content were used for the analysis of the quantitative determination of phenolic compounds. The presence of high phenolic, flavonoid and flavonol content in the extracts have contributed directly to the antioxidant activity. The antioxidant ability and radical scavenging properties of plants were also measured using three different assays, namely DPPH, RPA and FRAP. The leaves extract also exhibited remarkable antioxidant potentials as compared to the reference standard- ascorbic acid (AA) and butylated hydroxytoluene (BHT). Based on the results obtained in this study, the G. marantina leaves are the most effective antioxidants as determined through different in vitro assays which could be a potential source of natural antioxidants for use in food, cosmetics, and pharmaceutical industries.

Keywords: Globba marantina, Phytochemical screening, Phenolic compounds, Antioxidant activity.

Location: Chattogram University, Chattogram, Bangladesh and Bangladesh National Herbarium, Mirpur, Dhaka.

Start Date:

End Date:

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants

Objectives:

The present study is to examine the preliminary phytochemical screening and to evaluate the antioxidant potentials of the methanolic extract of Globba marantina leaves.

Methodology:

For this present investigation, the aforementioned medicinal plant was collected from Chattogram University, Chattogram, Bangladesh and was identified by Bangladesh National Herbarium, Mirpur, Dhaka. The accession number of, G. marantina is DACB-43650. The collected leaves were rinsed, cut into small pieces and dried under at room temperature for 10to12 days. The leaves were ground into a fine powder using an electric blender. About 400 gm of powdered material of plant was taken in a clean, flat bottomed glass container and soaked in 1600 ml of 90% methanol. The container with its contents was sealed and kept for a period of 15 days accompanying occasional shaking and stirring. The whole mixture then underwent a coarse filtration by a piece of clean, white cotton material. Then it was filtered through Whatman filter paper. The filtrate (methanol extracts) obtained were concentrated under reduced pressure at 40 ºC by using a rotary evaporator. The crude extracts were then dried in a freeze dryer, weighed and stored in desiccators until further use. Testing of different chemical groups present in the extract represents the preliminary phytochemical studies. A small quantity of partition of methanolic extract of leaves of Globba marantina were subjected to preliminary qualitative phytochemical investigation for detection of phytochemicals such as alkaloids, cardiac glycosides, anthraquinone, steroids, saponins, using the standard methods ^[13]. The detection was carried out based on visual observations of a color change or the formation of precipitates after the addition of specific reagents. In this study, the results were expressed as mean ± standard deviation of at least three replicates. Statistical analyses were carried out using Statistical Package for the Social Sciences (SPSS, IBM Corp., Armonk, NY, USA). Tukey’s pairwise comparison (HSD) and least significant difference (LSD) tests were performed to analyze variance and determine significant differences among mean values (P<0.05) ^[10].

Source of Information: Journal of Medicinal Plants Studies 2019; 7(6): 147-153

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Achievement/Findings:

The result of this study shows the presence of potent phytochemicals such as alkaloids, steroids, and cardiac glycosides in methanolic leaf extracts of G. marantina. The leaves extract of G. marantina also possesses a high content of phenolic compounds. The present study also revealed that the leaves extracts have moderate antioxidant activity in comparison with the reference standard. Thus, it can be concluded that its antioxidant properties might be due to the presence of high content of phenolic compounds and other phytochemicals present in this plant. So, the isolation of bioactive constituents from this plant might be a potential alternative for synthetic antioxidants.

Knowledge Management: Journal