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Variation of Semen Quality Among Three Generations (f1, F2 and F3) of Holstein FRIesian Upgraded Breeding Bulls of Bangladesh

Faruk Hossain
Lal Teer Livestock Development (BD) Limited, Mymensingh, Dhaka, Bangladesh

Md Golam Sorowar
Lal Teer Livestock Development (BD) Limited, Mymensingh, Dhaka, Bangladesh

Sharmin Akter Suma
Faculty of Animal Husbandry, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Abdullah Al Mansur
Lal Teer Livestock Development (BD) Limited, Mymensingh, Dhaka, Bangladesh

Md Mahbubul Hoque
Lal Teer Livestock Development (BD) Limited, Mymensingh, Dhaka, Bangladesh

Quazi M Emdadul Huque
Lal Teer Livestock Development (BD) Limited, Mymensingh, Dhaka, Bangladesh

Abstract/ Executive Summary:

The experiment was conducted in a well-known private livestock research and development farm in Bangladesh to find out the variation of semen quality parameters among three generations (F₁, F₂ and F₃) of Holstein Friesian upgraded breeding bulls. A total of 312 ejaculates were collected from 6 upgraded breeding bulls throughout the experimental year. The recorded data were summarized using Microsoft Excel 2010 and analyzed using Graph Pad Prism 5 software. Out of the 312 ejaculates, 273 (87.50%) were found to be creamy in color followed by 26 (8.33%) and 13 (4.17%) as yellowish and watery, respectively. A generation had a significant (P<0.05) effect on ejaculate volume, consistency, mass activity, sperm concentration, initial and post freezing motility. The highest (7.389±0.19ml) and the lowest (5.156±0.13ml) volume of semen were found in third (F₃) and first-generation (F₁), respectively. The mass activity ranges from 3.74±0.04 to 4.30±0.05. Sperm concentration and pH varied insignificantly (p>0.05) but initial motility and post-freezing motility had significant differences among the three generations. Initial motility ranges from 75.87±0.32 to 78.40±0.38 percent and the post freezing motility ranges from 50.38±0.41 to 52.16±0.43 percent. It could be concluded that most of the semen quality parameters were influenced by generation and freezing. Semen characteristics were better in F₂ followed by F₃ and F₁ generation in upgraded Holstein Friesian bulls.

Keywords: Breeding Bulls, Generation, Semen Quality, Holstein Friesian

Location: Lal Teer Livestock Development (BD) Limited, Mymensingh, Dhaka, Bangladesh

Start Date: 00-00-2019

End Date: 00-00-2019

Program Area: Animal Health and Management

Commodity: Bull

Objectives:

To assess the variation of semen quality among generations within the genotype (Holstein Friesian).

Methodology:

The study was undertaken at the research and development unit of a renowned research-based animal breeding organization of Bangladesh, known as “Lal Teer Livestock Development Bangladesh Limited”, located at Mymensingh district, around 90 kilometers away from Dhaka City. The experiment was carried out throughout the year 2019. Total 6 (six) breeding bulls from three generations (F₁, F₂ and F₃) of upgraded Holstein Friesian (nearly 28 to 52 months of age and bodyweight of 426.00 to 683.50 kg) were selected for this study. Out of 6 bulls, 2 were Holstein Friesian × Local (HF×L), 2 were Holstein Friesian× Holstein Friesian × Local (HF₁ ×L) and 2 were Holstein Friesian × Holstein Friesian × Holstein Friesian × Local (HF₂ × L) bulls. The breeding bulls were maintained under optimal feeding and management during the whole period of the experiment. The bulls were physically fit, free from diseases, clinically normal and sound in breeding. All the bulls were vaccinated against Anthrax, FMD, BQ, and HS according to the schedule. The bulls were allowed ad libitum green grass supplemented with good quality concentrate mixture prepared with maize grain, rice polish, wheat bran, soybean meal, mastered oil cake, DCP, vitamin-mineral premix and common salt. Semen was collected early in the morning twice a week from the bulls using sterilized bovine artificial vagina (IMV model-005417) maintaining proper temperature (42°- 45 °C), pressure and softness (Arthur et al., 1982). A male dummy was used for jumping the bulls and after 2 to 3 false jumps semen was collected from each bull by a skilled semen collector. Just after collection, each ejaculate was placed into a hot water bath at 37°C. Ejaculate volume of semen was measured directly in milliliter (ml) from the graduated centrifuge collection tube. Color and consistency of semen were observed with the naked eye. Semen pH was determined by indicator paper strips (Salisbury et al., 1978). The mass activity of semen was recorded by placing a small drop of fresh semen on the glass slide without coverslip under low magnification (10x) of a digital microscope and graded from 0 to 5 grades. The concentration of sperm per ml of semen was estimated through bovine sperm photometer (IMV technologies, France). Individual motility of semen was assessed by placing a small drop of semen on the glass slide and covering by coverslip under high magnification (40x) using a phase-contrast microscope. Semen with motility of more than or equal to 70% was diluted with an egg yolk-citrate-glycerol semen extender. The diluted semen was subsequently loaded in 0.25 ml/straw (IMV technologies, France), cooled at 4°C and equilibrated for 3.5 to 5 hours. Semen straw was then frozen using IMV bio-freezer following the standard procedure of IMV technologies. After that, frozen straws were transferred into liquid nitrogen until used for insemination. Post-freezing motility of semen was assessed as individual motility. The recorded data was compiled by Microsoft Excel 2010. Compiled data was then analyzed using GraphPad Prism 5 software. Column statistics were done for mean and standard error. Tukey test was performed for multiple comparison tests and level of significance.

Source of Information: Res. Agric., Livest. Fish.7(3): 457-463, December 2020

Article Link (Online Journal): DOI: https://doi.org/10.3329/ralf.v7i3.51365

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

In conclusion, it was evident that ejaculate volume, concentration, p^H and motility of sperm were influenced by freezing and generation of the genotype. Semen characteristics were better in F₂ followed by F₃ and F₁ generation of upgraded Holstein Friesian breeding bulls.

Knowledge Management: Journal