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Suppression Subtractive Hybridization Technique in Wheat for the Identification of Disease Resistance Differentially Expressed Genes

Md. Aktar Uz Zaman
Senior Scientific Officer
Pulses Research Centre, BARI, Ishurdi, Pabna

Mst. Tuhina-Khatun
Senior Scientific Officer
Bangladesh Rice Research Institute

Abstract/ Executive Summary:

A resistant variety with high yielding potential is key for increasing crop production to fulfill the food requirement of the ever-increasing world populations. Consequently, the aim of plant breeders is to develop high yielding varieties or cultivars that are resistant or tolerant to specific diseases or insects. For developing a resistant variety, it is enormously indispensable to incorporate or introgress the specific resistant genes of that particular disease into the recipient. Suppression subtractive hybridization (SSH) is a powerful technique for the identification of disease-specific differentially expressed genes that are expressed in a resistant or susceptible variety. This paper presents a brief review on the SSH technique with examples focusing on the identification of the wheat disease-specific differentially expressed genes and their defense mechanisms against fungal pathogens in global wheat cultivars. This review is helpful for wheat researchers for the updated information on the SSH technique for the identification of differentially expressed genes in the global wheat cultivars and varieties. Eventually, the identified genes could be used to develop the disease resistance variety through marker-assisted backcrossing programme or conventional breeding.

Keywords: Differentially expressed genes, expressed sequence tags, Resistance variety, Suppression subtractive hybridization, Triticum aestivum

Location: Universiti Putra Malaysia

Start Date: 03-03-2015

End Date: 12-06-2015

Program Area: Pest Management

Commodity: Durum wheat, Diseases

Objectives:

This paper presents a brief review on the SSH technique with examples focusing on the identification of the wheat disease specific differentially expressed genes and their defense mechanisms against fungal pathogens in global wheat cultivars.

Methodology:

This is a review paper and this article was written based on the secondary sources.

Source of Information: mmhanafi@agri.upm.edu.my

Article Link (Online Journal): Pertanika Journal of Scholarly Research Reviews http://www.pjsrr.upm.edu.my/

Funding Source:

1. Islamic Development Bank

Budget:

Total Budget (Tk.) :

Achievement/Findings:

This review presents a good number of differentially expressed genes identified by wheat researchers using the SSH technique against different fungal diseases of wheat. The updated information will be helpful to further design the experiments to develop durable disease-resistant varieties against fungal pathogen through marker-assisted backcrossing (MABC) or gene transformation through genetic engineering. These could be planned against a specific pathogen, or a combination of pathogens using gene pyramiding.

Knowledge Management: Journal