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Identification, Prevalence and Antimicrobial Susceptibility of Major Pathogenic Bacteria in Exportable Shrimp (penaeus Monodon) of Southern Bangladesh

Bibhas Talukder
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Flora Chakma
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abdullah Al Momen Sabuj
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Zobayda Farzana Haque
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Md. Tanvir Rahman
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Sukumar Saha
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

A total of 30 samples of shrimp were collected from two local sea food processing plant of Rupsha and Mongla Bazar of Khulna district to investigate the microbiological quality. Microbial quality was assessed by total viable count (TVC), total Vibrio count (TViC), total Staphylococcal count (TSC) and total E. coli count (TEC) by inoculating into specific media and the values of all the samples were ranged from log 2.92 to 3.41cfu/gm, log 2.06 to 2.11 cfu/gm, log 2.21 to 2.49 cfu/gm and log 2.14 to 2.22 cfu/gm respectively. Out of 30 samples 10, 11 and 5 samples were found positive for Vibrio spp., Staphylococcus spp. and. E. coli respectively. Results of antibiotic susceptibility test against eight commercially available antibiotics showed all three bacteria were multidrug-resistant. This study revealed that the shrimp samples of Mongla contain a higher load of bacteria than the samples from Rupsha.

Keywords: Shrimp, Microbial quality, Antibiotic sensitivity

Location: Southern Bangladesh

Start Date:

End Date:

Program Area: Pest Management

Commodity: Shrimp, Bacteria

Objectives:

The present study was designed to assess the microbial load and identification of the bacteria with their antimicrobial susceptibility status from shrimp processed at Shrimp processing plant at Khulna region.

Methodology:

Thirty samples were collected from two Shrimps processing plant (Rupsha and Mongla) of Khulna Region. After transporting to the laboratory in ice samples were processed and total viable count (TVC), total Staphylococcal count (TSC), total E. coli count (TEC) and total Vibrio count (TViC) were performed three times for each sample according to ISO. The representative bacterial colonies were characterized morphologically using Gram’s staining. Catalase test, Coagulase test, Sugar fermentation test, Indole test, Methyl red test (MR) and Voges-Prouskauer (VP) test were conducted according to the method described by Cheesbrough. All the isolates of Staphylococcus spp., Vibrio spp. and E. coli were tested for antimicrobial susceptibility test against eight commonly used antibiotics by disc diffusion or Kirby-Bauer method [8] and the results were compared with the standard. The data on total viable count (TVC), total Vibrio count (TViC), total Staphylococcal count (TSC) and total E. coli count obtained from the bacteriological examination were analyzed in a completely randomized design (CRD) using computer package subjected to analysis of variance using SPSS Software (Version 16, 2007). 2.2 Isolation and identification of bacteria In the current study, three different genera of bacteria including 10 of Vibrio spp., 5 of E. coli and 11 of Staphylococcus spp. were isolated and identified. Isolation was performed on their selective agar media. Thiosulphate citrate bile salt sucrose (TCBS) agar was used to isolate the Vibrio spp. where it appears as yellow, round and smooth colony that is characteristically similar to findings of Islam et al. The colony characteristics of E. coli observed on eosin methylene blue (EMB) agar were smooth, circular, black color with metallic sheen and Staphylococcus spp. on mannitol salt (MS) agar showed yellow, round and convex colony respectively. The cultural characteristics of E. coli and Staphylococcus spp. observed by Konuku et al. and Sharada et al. was similar to the study. Gram’s staining and biochemical test were used to identify the bacteria. In Gram’s staining, Vibrio spp. appeared as single curved shaped rod, E. coli showed pink color, small shaped gram negative and Staphylococcus spp. was cocci shape arranged in grapes like cluster. Islam et al. Thomas et al. and Brooks et al. also recorded similar staining characteristics of Vibrio spp., E. coli and Staphylococcus spp. All the isolates of Vibrio spp. produced acid by fermenting dextrose, maltose, sucrose and mannitol except lactose. Vibrio spp. also gave positive reaction to methyl red (MR) and indole production tests and negative reaction to Voges– Proskauer (VP) test. Staphylococcus spp. was found catalase and coagulase positive whereas E. coli fermented dextrose, lactose, maltose, sucrose and mannitol with the production of gas and acid. The results of biochemical test agreed with the findings of the previous researcher.

Source of Information: International Journal of Fisheries and Aquatic Studies 2019; 7(1): 96-99

Article Link (Online Journal):

Funding Source:

1.

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Considering the findings of this research work, it may be concluded that Vibrio spp.; Staphylococcus spp. and E. coli were successfully isolated and confirmed by different cultural media, biochemical tests. Isolated bacteria showing multidrug resistance for commonly used antibiotics are alarming. The concentrations of bacteria in shrimp of Rupsha were lower than the shrimp of Mongla. The presence of E. coli in shrimp indicated the maintenance of low hygienic conditions during processing. So, the processing of shrimp must be performed in a standard hygienic way from the onset of the handling to minimize the incidence of microbial contamination and the effect of multidrug resistance on human health.

Knowledge Management: Journal