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Screening of Organic Composts for Mass Culturing of Trichoderma Harzianum to Be Used Against Soil-borne Pathogen Sclerotium Rolfsii and Fusarium Oxysporum of Lentil

MD. IQBAL FARUK
PSO
Plant Pathology Division, BARI, Gazipur

Abstract/ Executive Summary:

The experiment was conducted to observe the suitability of organic compost and vermicompost for mass culturing of a bio-control agent, Trichoderma harzianum and its effectiveness against foot and root rot disease of lentil caused by Sclerotium rolsfii and Fusarium oxysporum. The formulated T. harzianum cultured in two different compost viz. organic compost and vermin-compost is designated as Tricho-organic-compost and Tricho-vermicompost. The present study revealed that soil amendment with Tricho-vermicompost and Tricho-organic-compost are considered to be the best treatments in reducing seedling mortality and in increasing the yield of lentil. Seed treatment with a chemical fungicide such as Provax showed better performance against the disease and its effect at per the soil amendments with organic compost and vermin-compost in reducing seedling mortality and increasing plant growth and yield of lentil.

Keywords: Vermicompost, Trichoderma harzianum, Mass culture, Foot and root rot disease,. Lentil

Location: The experiment was conducted in the field of Plant Pathology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipu

Start Date: 00-00-2017

End Date: 00-00-2018

Program Area: Pest Management

Commodity: Diseases, Lentil

Objectives:

The present research was under taken to find out the effective formulation of T. harzianum against foot and root rot disease of lentil.

Methodology:

The experiment was conducted in the field of Plant Pathology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur. There were 6 treatments viz. (i) Seed treatment with Provax (ii) Soil amendment with organic compost (iii) Soil amendment with Tricho-organic compost (iv) Soil amendment with vermicompost and (v) Soil amendment with Tricho-vermicompost and (vi) Untreated control. The unit plot size was 2 m x 3m. RCB design was followed with 3 replications. T. harzianum was multiplied in the mixture of Grasspea and wheat bran with mustard oilcake substrates. The formulated Trichoderma was used to mass multiplication in two different compost viz. organic compost and vermin-compost and its designated Tricho-organic-compost and Tricho-vermicompost. T. harzianum was cultured in PDA media and spores were harvested 10 days old cultured and were used for seed treatments. The soil was inoculated with S. rolfsii colonized substrates @ 100g/m² soil before seed sowing. Tricho-composts were added @ 3 t/ha after inoculation with pathogens. Seeds were treated with Provax @ 2.5 g/kg seeds before seed sowing. BARI Masur-5 variety was used in this study. Data were collected on seedling mortality; shoot height, shoot weight, root length and root weight. The yield of lentil was also recorded. Data were analyzed statistically by using the MSTATC program. The treatment effects were compared following the least significant difference (LSD) test (P=0.05).

Source of Information: Annual Research Report 2017-18, Plant Pathology Division, BARI, Gazipur

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

From this, it may be concluded that soil amendment with Tricho-organic-compost and Tricho-vermicompost is to be the best treatment for management foot and root rot disease of lentil and getting a higher yield of lentil than control. Seed treatment with chemical fungicide Provax also a better treatment for management foot and root rot disease of lentil.

Knowledge Management: Report/Proceedings