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Collection, Isolation and Screening of Indigenous Rhizobial Strains for Different Legumes (2018–2019)

M.E. Ali
Soil Science Division, BARI, Joydebpur, Gazipur, Bangladesh

F. Alam
Soil Science Division, BARI, Joydebpur, Gazipur, Bangladesh

M.F.A. Anik
Soil Science Division, BARI, Joydebpur, Gazipur, Bangladesh

M. Rahman
Soil Science Division, BARI, Joydebpur, Gazipur Bangladesh

S. Akhter
Soil Science Division, BARI, Joydebpur, Gazipur Bangladesh

Abstract/ Executive Summary:

Field experiments on biofertilizer were carried out at Multi-Location Testing site, Tularampur, Narail on lentil during rabi 2016-2017,2017-2018 and 2018-2019; Farming System Research & Development (FSRD) site, Hatgobindapur, Faridpur on lentil and groundnut during rabi 2017-2018 and 2018-2019 with the objectives i) to evaluate the response of lentil and groundnut to biofertilizer under farmers’ field condition, and ii) to motivate uses of biofertilizer instead of N-fertilizer for a pulse and oilseed cultivation. The experiment was laid out in RCBD with 4/6 dispersed replications. Unit plot size was 15 m x 10 m. Three fertilizer treatments viz. T₁: 22-42-20-5 kg P-K-S-Zn ha^-1, T₂: 22-42-20-5 kg P-K-S-Zn ha^-1 + Rhizobium Inoculum and T₃: 50-22-42-20-5 kg N-P-K-S-Zn ha^-1was studied. BARI Masur-6 of lentil, BARI Chinabadam-4 of groundnut and peat-based rhizobial inoculum (strain BARI RLc-104 for lentil and strain BARI RAh-803 for groundnut @ 1.5 kg ha^-1 were used in the demonstration trial. The experimental result revealed that the application of biofertilizer along with PKSZn produced higher seed yield and yield attributes of lentil at Narail, lentil and groundnut at Faridpur. It is evident from the experiment that the application of biofertilizer instead of applying nitrogenous fertilizer can achieve a higher yield of lentil at farmers’ fields in Narail and lentil and groundnut in Faridpur. Higher BCR was noted in T₂ treatment where Rhizobium plus chemical fertilizers (PKSZn) were used.

Keywords: Lentil, Biofertilizer, N-fertilizer, Legumes

Location:

Start Date: 00-00-2018

End Date: 00-00-2019

Program Area: Crop-Soil-Water Management

Commodity: Bio fertilizer, Lentil

Objectives:

The present study was, therefore under taken i) to evaluate the response of some pulse crops to biofertilizer under farmers’ field condition, and ii) to reduce the uses of N-fertilizer for lentil cultivation.

Methodology:

The experiment was laid out in randomized complete block design having six replications with three treatments. The unit plot size was 15m × 10 m. The variety BARI Masur-6 of lentil and BARI Chinabadam-4 of groundnut and peat-based rhizobial inoculum (strain BARI RLc-104 for lentil, strain BARI RAh-803 for groundnut @ 1.5 kg ha^-1 were used in demonstration trial. There were three treatments viz. T₁: 22-42-20-5 kg P-K-S-Zn ha^-1, T₂: 22-42-20-5 kg P-K-S-Zn ha^-1 + Inoculum, T₃: 50-22-42-20-5 kg N-P-K-S-Zn ha^-1 was studied. The above peat-based rhizobial inoculum was used @ 1.5 kg ha^-1 containing about 10⁸ cells g^-1 inoculum. Chemical fertilizers i.e. P, K, S and Zn were applied in all treatments as basal dose @ 22 kg P ha^-1 from TSP, 42 kg K ha^-1 from MP, 20 kg S ha^-1 from gypsum and 5 kg Zn ha^-1 from zinc sulphate for lentil, chickpea. In T₃ treatment, 50-22-42-20-5 kg N-P-K-S-Zn ha^-1 for lentil and 100-22-42-40-5 kg N-P-K-S-Zn ha^-1 for groundnut were used. Lentil seeds were sown in line maintaining 30 cm from line to line distance during on 15-22 November 2016, 2017 and 2018 at Narail, 22 November 2017, 27-28 November 2018 at Faridpur and the groundnut seeds were sown in line maintaining the spacing of 40 cm x 10 cm during 14 March 2019 at Faridpur. The rhizobial population of Rhizobium inoculant was about 10⁸ cells g^-1 of inoculant. During the course of the experiment, the growth and development of plants in the field were carefully observed. Lentil was harvested on 07-15 March 2018 at Narail, 7-9 March 2019 at Faridpur, Groundnut was harvested on 03 July 2019 at Faridpur. All the data were analyzed statistically.

Source of Information: Annual Research Report 2018–2019, Soil Science Division, BARI, Joydebpur, Gazipur Bangladesh

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The result of biofertilizer in lentil production seems to be promising in AEZ # 12. Similar results were observed in the previous year. The highest seed yield obtained from plants treated with P₂₂K₄₂S₄₀Zn₅kg ha^-1chemical fertilizer along with Rhizobium inoculum (BARI Rah-803) might be suitable and economically viable for groundnut cultivation in Faridpur region under the Low Ganges River Floodplain (Agroecological Zone 12).

Knowledge Management: Report/Proceedings