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Response of Lentil Varieties to Elite Strains of Rhizobium (2018–2019)

M.E. Ali
Soil Science Division, BARI, Joydebpur, Gazipur, Bangladesh

M.F.A. Anik
Soil Science Division, BARI, Joydebpur, Gazipur, Bangladesh

F. Alam
Soil Science Division, BARI, Joydebpur, Gazipur, Bangladesh

M. Rahman
Soil Science Division, BARI, Joydebpur, Gazipur, Bangladesh

Abstract/ Executive Summary:

The field experiment was conducted at the research field of Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, during 2018-2019 with the objectives to study the response of Rhizobium inoculation with different varieties of BARI released lentil. Four varieties of lentil viz. BARI Masur-6, BARI Masur-7, BARI Masur-8 and BARI Masur-9, and rhizobial inoculum (Rhizobium strain RLc-10⁴) were used in these experiments. Unit plot size was 4 m x 3 m. The experiment was designed in the randomized complete block having 3 replications in each treatment. Each variety was tested with/without Rhizobium inoculation. Inoculated plants gave significantly higher nodule number, nodule weight, shoot weight and seed yield compared to non-inoculated plants. Among 4 varieties, BARI Masur-8 produced the highest nodule number and nodule weight. The highest seed yield (1.02 t ha^-1 ) was recorded in BARI Masur-7. The interaction effect revealed that the highest seed yield of 1.12 t ha^-1 was recorded by inoculated BARI Masur-7.

Keywords: Varieties, Lentil, Inoculation, Fertilizer, Rhizobium

Location: Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh

Start Date: 00-00-2018

End Date: 00-00-2019

Program Area: Variety and Species

Commodity: Lentil

Objectives:

The present investigation was undertaken to study the response of inoculation with different varieties of lentil.

Methodology:

Lentil was sown on 19 November in 2018 at the research field of Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur. The experiment was designed in the randomized complete block having 3 replications in each treatment. Four varieties of lentil viz. BARI Masur-6, BARI Masur-7, BARI Masur-8 and BARI Masur-9 and rhizobial inoculum (Rhizobium strain RLc-104) were used in these experiments. Each variety was treated with or without Rhizobium inoculant. Unit plot size was 4 m × 3 m. There were 8 treatment combinations. Basic doses of phosphorus @ 22 kg P ha^-1as TSP, potash @ 42 kg K ha^-1 as muriate of potash, sulphur @ 20 kg S ha^-1as gypsum and zinc @ 5 kg Zn ha^-1 as zinc oxide were applied in the field. Urea was applied neither in the inoculated nor in the non-inoculated plots. Peat based rhizobial inoculum (Rhizobium strain RLc-104) @ 1.5 kg ha^-1 was used for seed inoculation. Peat based rhizobial inoculum was used containing about 10⁸ cells g^-1 inoculum. During the course of the experiment growth and development of plants in the field were carefully observed. At the 50% flowering stage, 10 randomly selected plants were uprooted from each unit plot. The dry weight of roots, shoots and nodules including nodule numbers were recorded. The plants were harvested on 20 March 2019. Data on plant height, 1000-seed weight (g), No. of pods plant^-1, No. of seeds pod^-1, seed yield and stover yield was also taken. All data were analyzed statistically. Soil pH was measured by a combined glass calomel electrode (Jackson, 1958). Organic carbon was determined by the wet oxidation method (Walkley and Black, 1934). Total N was determined by modified Kjeldahl method. Calcium, K and Mg were determined by NH₄OAc extraction method. Copper, Fe, Mn and Zn were determined by DTPA extraction followed by AAS reading. Boron was determined by the CaCl₂ extraction method. Phosphorus was determined by the Modified Olsen method (Neutral + Calcareous soils). Sulphur was determined by CaH₄(PO₄)₂.H₂O extraction followed by turbidimetric turbidity method with BaCl_2.

Source of Information: Annual Research Report 2018–2019, Soil Science Division, BARI, Joydebpur, Gazipur, Bangladesh

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

From the first-year trial, it may be concluded that the highest seed yield of 1.12 t ha^-1 was recorded by inoculated BARI Masur-7. This experiment should be continued for next year.

Knowledge Management: Report/Proceedings