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Research Detail

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F. ALAM
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh

M. J. ALAM
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh

M. A. H. BHUIYAN
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh

A field experiment was conducted at Central Farm, Regional Agricultural Research Station, Jessore on 10 December 2016 on tomato with the objectives to study the effect of combined use of arbuscular mycorrhiza and phosphorus on tomato and to reduce to use of P-fertilizer under field condition. The experiment was designed in factorial RCBD with six treatments and four replications. The tomato variety was BARI Tomato-15 as test crop. Soil-based arbuscular mycorrhizal (AM) inoculum was used in the seedbed for this experiment containing about 100 spores and infected root pieces of the host plant which was used at the rate of 1 kg m-2. The treatment combinations were: T1P1U: 0% P ´ Without AM, T2P2U: 50% P ´ Without AM, T3P3U: 100% P ´ Without AM, T4P1AM: 0% P ´ With AM, T5P2AM: 50% P ´ With AM, T6P3AM: 100% P ´ With AM. Mycorrhizal inoculation increased plant height (cm), collar diameter(cm), root length(cm), shoot dry weight (g), root dry weight (g), root colonization (%), spore population (100g-1 soil), number of fruit plant-1, fruit length (cm), fruit diameter (cm), fruit volume (cm3), fruit weight (g fruit-1) fruit weight (kg plan-1) and fruit yield (t ha-1). The plant that received AM in the nursery bed produced a higher yield than without AM in all phosphorus levels. The highest tomato yield (43.7 t ha-1) was recorded in 50% P plus AM (AM was used in nursery bed). AM inoculated plant with 50% P gave 43.7 t ha-1 yield of tomato while non-AM inoculated with 100% P gave 36.8 t ha-1 yield which indicates that inoculation of AM used in nursery bed can save 50% P in the field. The plant which did not receive AM in the nursery bed produced a lower yield in all phosphorus levels in the field. Further study in P deficient soil and economic analysis is required for final recommendation.

  Arbuscular mycorrhiza, Phosphorus, Tomato, P-fertilizer, Fertilizer
  Central Farm, Regional Agricultural Research Station (RARS), Jessore, Bangladesh
  00-00-2015
  00-00-2016
  Crop-Soil-Water Management
  Fungal Disease, Tomato

To study the effect of combined use of arbuscular mycorrhiza and phosphorus on tomato and to reduce to use of P-fertilizer under field condition. 

A field experiment was carried out on 10 December 2016 for tomato during rabi season at Central Farm, Regional Agricultural Research Station (RARS), Jessore. The experiment was laid out in Factorial Randomized Complete Block Design (RCBD) with four replications. There were six treatment combinations viz. T1P1U: 0% P ´ Without AM, T2P2U: 50% P ´ Without AM, T3P3U: 100%  P ´ Without AM, T4P1AM: 0% P ´ With AM, T5P2AM: 50% P ´ With AM, T6P3AM: 100% P ´ With AM. The tested crop was tomato (cv. BARI Tomato-15). The unit plots measured 3 m × 3 m in size. Soil-based arbuscular mycorrhizal (AM) inoculum was used in the seedbed for producing tomato seedlings containing about 100 spores and infected root pieces of the host plant was used at the rate of 1 kg m-2 in the seedbed. A layer of AM inoculum was firstly placed in each bed and was covered with a thin soil layer of 1 cm. Seeds were sown in seedbed and transplanted after 30 days in the field. The physical and chemical properties of the initial soil sample from seedbed and experimental fields at a depth of 0-15 cm were collected and analyzed following standard methods and presented. 

Soil pH was measured by a combined glass calomel electrode (Jackson, 1958). Organic carbon was determined by the wet oxidation method (Walkley and Black, 1947). Total N was determined by modified Kjeldahl method. Calcium, K and Mg were determined by NH4OAc extraction method. Copper, Fe, Mn and Zn were determined by DTPA extraction followed by AAS reading. Boron was determined by CaCl2 extraction method. Phosphorus was determined by Modified Olsen method (Neutral + Calcareous soils). Sulphur was determined by CaH4(PO4)2.H2O extraction followed by turbidimetric turbidity method with BaCl2. Nitrogen, phosphorus, potassium, sulphur, zinc and boron were used in the form of urea, TSP, MoP, gypsum, zinc sulphate and boric acid, respectively. Recommended fertilizer dose (BARC, 2012) for tomato (160 kg N, 30 kg P, 60 kg K, 13 kg S, 1.5 kg Zn and 0.5 kg B kg ha-1) were used. Phosphorus was used in three levels (0% P, 50% P and 100% P). All K, S, Zn, B and 1/3rd amount urea were applied at the time of final land preparation and the remaining 2/3rd amount of urea-N were applied in two equal installments at 25 and 45 days of transplanting. All the intercultural operations such as irrigation, sticking, weeding, insect control, etc. were done as and when necessary. Data on yield and yield components were recorded at maturity. The tomato was harvested from 16 March to 20 April 2016. The initial soil samples at a depth of 0-15 cm from the experimental fields were collected and analyzed following standard methods. 

Statistical analyses were conducted using standard statistical procedures (Gomez and Gomez, 1984) implemented in Statistix 10. The data were examined by analysis of variance (ANOVA). Differences between the treatments were determined by ANOVA, and Fisher’s protected least significant difference (LSD) was calculated at the 0.05 probability level for treatment mean comparisons.

  Annual Research Report 2015-2016, Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh
  
Funding Source:
1.   Budget:  
  

Mycorrhizal inoculated plant with 50% phosphorus produced higher root colonization, growth parameters, yield, and yield attributes compared to the non-inoculated plant. AM inoculated tomato plant with 50% phosphorus gave 43.7 t ha-1 yield while non-AM inoculated with 100% phosphorus gave 36.8 t ha-1 which indicates that inoculation of AM in a nursery bed and transplanted to the field can save about 50% P in the field. 

  Report/Proceedings
  


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