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Research Detail

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M.E. Ali
Soil Science Division, BARI, Joydebpur, Gazipur, Bangladesh

M.F.A. Anik
Soil Science Division, BARI, Joydebpur, Gazipur, Bangladesh

M. Rahman
Soil Science Division, BARI, Joydebpur, Gazipur, Bangladesh

F. Alam
Soil Science Division, BARI, Joydebpur, Gazipur, Bangladesh

S. Akhter
Soil Science Division, BARI, Joydebpur, Gazipur, Bangladesh

The experiment was carried out during the Rabi season of 2019-2020 in the research field of Soil Science Division, BARI, Joydebpur, Gazipur to find out the effect of Azotobacter inoculum along with different doses of N fertilizer on the growth and yield of Onion. The experiment was designed in RCBD with 6 treatments and 4 replications. Onion (BARI piaj-4) was used as a test crop. Liquid azotobacter inoculum was used in this experiment. The population density of the used inoculum was more than 108 cells ml-1 liquid inoculant. There were six treatments viz. T1: 100% N of Recommended Dose, T2: 90% N + Azotobacter inoculum, T3: 80% N + Azotobacter inoculum, T4: 70% N + Azotobacter inoculum, T5: Azotobacter inoculum and T6: Control. Results of the experiment revealed that the highest bulb yield of onion (23.75 t ha-1) was found in T3 treatments which were statistically identical with T1 (22.18 t ha-1) and T2 (23.60 t ha-1) treatments. This result suggested that the use of azotobacter inoculum in combination with a reduced dose of N fertilizer was beneficial for onion in the Grey Terrace soils of Gazipur (AEZ 28) and we could reduce 20% of nitrogenous fertilizer. The experiment should be continued for confirming the findings.

  Azotobacter, Growth, Yield, Onion
  Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh
  00-00-2018
  00-00-2019
  Crop-Soil-Water Management
  Micronutrient

To find out the most effective dose of Azotobacter for growth and yield of onion (Allium cepa L.).

The experiment was carried out during the Rabi season of 2018-2019 in the research field of Soil Science Division, BARI, Joydebpur, Gazipur. Seedlings of onion (BARI piaj-4) were raised on the net house of Soil Science Division, BARI, Joydebpur, Gazipur. The silted (sandy clay loam) soils were collected from the bank of Turag river at Kodda, Gazipur was used in the seedbed. Cowdung was used at 1 kg m-2 in the seedbed. The pH of cow dung was 6.7 and the nutrient contents were: organic matter 14.1%, N 0.8%, P 1.26%, K 0.88%, Ca 1.55%, Mg 0.82%, S 0.62%, Fe 0.25% and Mn 0.112%. The soil contained 49 AM (100-1 g soil) spores of indigenous mixed AM fungal species and each gram of soil contain a population of rhizobium, azotobacter and phosphate solubilizing bacteria (PSB) was 4.5 x 104, 5.0 x 104 and 3.5 x 104, respectively. The experiment was conducted under unsterilized soil conditions. Soil pH was measured by a combined glass calomel electrode. Organic carbon was determined by the Wet Oxidation Method. Total N was determined by the modified Kjeldahl method. Calcium, K and Mg were determined by NH4OAc extraction method. Copper, Fe, Mn and Zn were determined by DTPA extraction followed by AAS reading. Boron was determined by the CaCl2 extraction method. Phosphorus was determined by the Modified Olsen method (Neutral + Calcareous soils) according to Olsen et al. (1954). Sulphur was determined by CaH4(PO4)2.H2O extraction followed by turbidimetric turbidity method with BaCl2. Chemical fertilizers @ 100 kg N ha-1 from urea, 55 kg P ha-1 from TSP, 75 kg K ha-1 from MoP, 22 kg S ha-1 from gypsum, 5 kg Zn ha-1 from ZnSO4 for onion were applied (FRG, 2018). All other fertilizers except urea-N and 2/3rd of MoP was applied as basal during final land preparation and urea-N was applied at three equal splits and 2/3rd of MoP was applied as two equal splits. Azotobacter inoculum was prepared following standard microbiological procedure. Azotobzcter strains were inoculated on Jensen’s N-free medium (Jensen 1951) slants. From the slants, the subculture was done by N-free Jensen’s medium (Jensen 1951). From the subculture plate, the pure culture of Azotobacter was inoculated in conical flux containing Jensen’s N-free broth (Jensen 1951). The broth was shaking in a horizontal shaker for 3-4 days for sufficient growth of the bacterial cells. Bacterial growth was determined by the plate count dilution method. After proper growth of azotobacter, the broth was used as Azotobacter inoculum. The seedling of the onion was soaked in the liquid inoculum for 2-3 hours. After soaking the seedling, the seedlings were transplanted in the field. The experiment was designed in RCBD with 6 treatments and 4 replications. There were six treatments viz. T1: 100% N of Recommended Dose, T2: 90% N + Azotobacter inoculum, T3: 80% N + Azotobacter inoculum, T4: 70% N + Azotobacter inoculum, T5: Azotobacter inoculum and T6: Control. A blanket dose of PKSZn was applied for each treatment. Healthy seedlings of onion (BARI piaj-4) were collected from the seedbed, net house of soil science division, BARI, Joydebpur, Gazipur. Six square meters (2m × 3m) plot was prepared in the experimental field of soil science division, BARI, Joydebpur, Gazipur. After preparing the experimental plot, seedlings were transplanted at a spacing of 20 cm × 10 cm. Onion seedlings were transplanted on 6th December 2019. Intercultural operations like watering, weeding, insect pests’ management etc, were done when necessary. Topdressing of urea and MoP fertilizer was done as per schedule. Five plants of onion from each plot were collected after 45 days after transplanting for recording some growth parameters like plant height, the number of leaves plant-1, shoot weight and root weight. Onion was harvested on 25th March 2020. Some parameters like plant height, individual bulb weight, bulb length, bulb diameter and bulb yield plot-1 were recorded. Data were statistically analyzed using Analysis of Variance (ANOVA) following CropStat package while all pair comparisons were done by Statistix 10.

  Annual Research Report 2019–2020, Soil Science Division, BARI, Joydebpur, Gazipur, Bangladesh
  
Funding Source:
1.   Budget:  
  

The use of Azotobacter inoculum in combination with nitrogenous fertilizer had a positive effect on the growth and yield attributes of onion. It also helps to reduce the use the chemical nitrogenous fertilizers which save money and maintain a safe soil environment. It should be continued for confirming the results.

  Report/Proceedings
  


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