M. E. Ali
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh
M.M. Masud
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh.
M. Rahman
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh.
M. F. A. Anik
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh.
F. Alam
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh
M. A. H. Bhuiyan
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh
Biochar, Biofertilizer, Peat, Plants
The central research field of Bangladesh Agricultural Research Institute and sawdust from sawmill, Gazipur, Bangladesh
Crop-Soil-Water Management
Bio fertilizer, Coal
Collection of organic materials and preparation of Biochar:
Six organic materials were selected for biochar preparation namely sawdust, rice straw, wheat straw, groundnut straw, shon grass and mimosa. The plants materials were collected from the central research field of Bangladesh Agricultural Research Institute and sawdust from sawmill. All the organic materials were air dried first. The plant materials were chopped into small pieces. The materials were burned separately using biochar preparation device. The biochar were ground and sieved. Fifty grams of each type of biochar materials were packed using polythene packet.
Treatments details:
T1: Rice straw based biochar
T2: Grass based biochar
T3: Sawdust based biochar
T4: Groundnut straw based biochar
T5: Mimosa based biochar
T6: Chickpea straw based biochar
T7: Peat
Characteristics of carrier materials:
All the carrier materials were analyzed for chemical compositions using standard chemical procedure. The chemical composition of different biochar materials and peat are given. The organic carbon content of biochar materials was higher over peat. The highest organic carbon content was found in sawdust based biochar and the highest N content was observed in lozzaboti based biochar. The pH value was around 8 in all the biochar materials where the peat shown the pH value of less than 5.
Preparation of Rhizobium broth culture and inoculation into the carrier materials:
We selected BARI RGm-901 (a Rhizobium strain isolated for soybean, Glycine max L.) as a Rhizobium strain for this experiment. Yeast manitol agar media was prepared and autoclaved. Then Rhizobium strain was inoculated to the media and shake it by using rotary shaker for 3-5 days. After sufficient growth of Rhizobium in the broth, the broth was injected in the carrier materials packet. Twenty mL of broth culture was injected in each of the packet. The bags were thoroughly kneeded to ensure absorption of liquid culture into the carrier. After injection of broth culture, the packets were kept in the incubator at 28°C temperature for 7 days. Then we stored the inoculums packet into two parts, one part in room temperature and the other was in fridge at 4°C. The inoculum packets were analyzed using standard microbiological procedure for rhizobial population count at 15, 30, 45, 60, 75 and 90 days after inoculation
Estimation of Rhizobium population:
The population of Rhizobium was enumerated by serial dilution of the samples upto 10-7, and plating in yeast manitol agar medium by following drop plate technique. Dilutions from 10-2 and 10-7 were plated by dividing the petriplates and labeling the dilutions accordingly. The population of the inoculant in the inoculum packets was estimated at periodic intervals upto 90 days. The results were expressed as cfu g-1 dry weight of the samples.
Annual Research Report 2016-2017, Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh.
Report/Proceedings