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Integrated Use of Arbuscular Mycorrhiza, Cowdung and Phosphorus in Producing Sweet Gourd Seedling (2016-2017)

M. F. A. Anik
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh

M. E. Ali
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh.

M. A. H. Bhuiyan
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh.

Abstract/ Executive Summary:

A pot experiment on the integrated use of arbuscular mycorrhiza, cowdung and phosphorus in producing sweet gourd seedlings was conducted in the Net house of Soil Science Division, BARI, Gazipur during rabi season of 2015-2016 and 2016-2017. Seven fertilizer treatments viz. T₁: Cowdung (CD), T₂: Arbuscular mycorrhiza (AM), T₃: phosphorus (P), T₄: CD + AM, T₅: P + AM, T₆: CD + P and T₇: CD + AM + P were studied along with T₈: Control for producing seedlings of this crop. Cowdung and TSP were used at the rate of 100 g and 1.0 g pot^-1, respectively. Soil based AM inoculum containing about 200 spores and infected root pieces were used in the pot of about 3 cm depth. Dry weight of seedlings, seedling height was found significantly higher with combined application of cowdung, arbuscular mycorrhiza (AM) and phosphorus compared to the remaining treatments.

Keywords: Arbuscular mycorrhiza, Cowdung, Phosphorus, Fertilizer, Sweet gourd, Seedling

Location: The net house of Soil Science Division, BARI, Gazipur, Bangladesh

Start Date: 00-00-2015

End Date: 00-00-2017

Program Area: Crop-Soil-Water Management

Commodity: Cowdung, Fertilizer, Sweet gourd

Objectives:

To observe the effect of combined application of arbuscular mycorrhiza, cowdung and phosphorus fertilizer on the performance of sweet gourd seedlings.

Methodology:

An experiment on the integrated use of arbuscular mycorrhiza, cowdung and phosphorus in producing sweet gourd seedlings was conducted in the pot at the net house of Soil Science Division, BARI, Gazipur during rabi season of 2015-2016 and 2016-2017. Soil from the bank of Turag River at Kodda, Gazipur was used in the pot. Chemical properties of the initial soil are presented. The soil was slightly alkaline in reaction. Organic matter, major nutrients and copper contents of the soil were low, while zinc and manganese levels were quite high. The pH of cowdung was 6.7 and the nutrient contents were: organic matter 14.1%, N 0.8%, P 1.26 %, K 0.88%, Ca 1.55%, Mg 0.82%, S 0.62%, Fe 0.25% and Mn 0.112%. The soil contained 18 AM spores of indigenous mixed AM fungal species and the experiment was conducted under non-sterilized soil condition.

Methods of chemical analysis:

Soil pH was measured by a combined glass calomel electrode (Jackson, 1958). Organic carbon was determined by wet oxidation method (Walkley and Black). Total N was determined by modified Kjeldahl method. Calcium, K and Mg were determined by NH₄OAc extraction method. Copper, Fe, Mn and Zn were determined by DTPA extraction followed by AAS reading. Boron was determined by CaCl₂ extraction method. Phosphorus was determined by Modified Olsen method (Neutral + Calcareous soils). Sulphur was determined by CaH₄(PO₄)₂.H₂O extraction followed by turbidimetric turbidity method with BaCl_2.

The experiments were laid out in randomized complete block design (RCBD) with four replications. Seven fertilizer treatments viz. T₁: Cowdung (CD), T₂: Arbuscular mycorrhiza (AM), T₃: Phosphorus (P), T₄: CD + AM, T₅: P + AM, T₆: CD + P and T₇: CD + AM + P were studied along with T₈: Control for producing sweet gourd seedlings. Cowdung and TSP were used at the rate of 100 g and 1.0 g pot^-1, respectively. No other fertilizers were used. The seeds were sown in 10 cm apart solid lines during 30 November in 2015-2016 and 2016-2017, respectively. Soil based AM inoculum containing about 200 spores and infected root pieces was used in the seed furrows of about 3 cm depth. A soil layer of about 1 cm thickness was spread on the inoculum layer on which the seeds were sown. BARI Misti kumra-1 was used as the varieties of sweet gourd. The seedlings were thinned down to about 3 cm from seedling to seedling within a week of germination. Watering, weeding and other intercultural operation were done as and when necessary. The seedlings were harvested on 05 and 08 January in 2015-2016 and 2016-2017. Data on dry weight of seedlings and yield components were recorded. Plant samples were oven dried at 70^oC until constant weight. Chemical analyses of the samples were done. Data were analyzed using the statistics-10 software.

Source of Information: Annual Research Report 2016-2017, Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh.

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It is evident from the results that performance of sweet gourd seedlings was greatly improved by combined application of cowdung, AM and P fertilizer. The biomass yield of this crop was improved with this treatment.

Knowledge Management: Report/Proceedings