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Response of Chickpea Varieties to Elite Strains of Rhizobium (2016-2017)

M. F. A. Anik
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh

M. H. R. Sheik
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh.

M. A. H. Bhuiyan
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh.

M. E. Ali
Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh.

Abstract/ Executive Summary:

Field experiments were conducted at Regional Agricultural Research Station, BARI, Ishurdi during 2015-2016 and 2016-2017 with the objectives to study the response of inoculation with different varieties and to study the effect of different sites (Agro-ecological zones) with inoculation and varieties. Four varieties/advance lines of chickpea viz. BARI Chola-9, BCX-06001-11, BCX-05001-4, BCX-05008-11 and rhizobial inoculum (Rhizobium strain RCa-220) were used in these experiments. Unit plot size was 4 m ´ 3 m. The experiment was designed in randomized complete block having 3 replications in each treatment. Each variety was tested with/without Rhizobium inoculation. Inoculated plants gave significantly higher nodule number, nodule weight, shoot weight, seed yield and stover yield compared to non-inoculated plants. Among 4 varieties/advance lines, BCX-06001-11 advance line produced the highest nodule number both in 2015-2016 and 2016-2017. The highest nodule weight recorded by BARI Chola-9 and BCX-05001-4 advance line in 2015-2016 and 2016-2017, respectively. The highest seed yield (2.22 t ha^-1) and stover yield (3.13 t ha^-1) was recorded at BCX-05008-11 advance line in 2015-2016 but in 2016-2017 growing season, the highest seed yield (1.84 t ha^-1) and stover yield (3.86 t ha^-1) were observed at BCX-05001-4 advance line. The interaction effect revealed that the highest seed yield of 2.62 and 1.98t ha^-1 and stover yield 3.60 and 4.16 t ha^-1 were recorded by inoculated BCX-05008-11 and BCX-05001-4 advance line of chickpea in 2015-2016 and 2016-2017 respectively.

Keywords: Inoculation, Agro-ecological zones, AEZ, Muriate of potash, Sulphur, Gypsum, Zinc

Location: Regional Agricultural Research Station, Ishurdi, Bangladesh

Start Date: 24-11-2015

End Date: 27-11-2016

Program Area: Crop-Soil-Water Management

Commodity: Chickpea, Bio fertilizer

Objectives:

To study the response of inoculation with different varieties and to study the effect of different sites (Agro-ecological zones) with inoculation and varieties.

Methodology:

Chickpea was sown on 24 November in 2015 and 27 November in 2016 at Regional Agricultural Research Station, Ishurdi. The experiment was designed in randomized complete block having 3 replications in each treatment. Four varieties/advance lines of chickpea viz. BARI Chola-9, BCX-06001-11, BCX-05001-4 and BCX-05008-11 were tested. Each variety was treated with or without Rhizobium inoculant. Unit plot size was 4 m × 3 m. There were 8 treatment combinations. Basic doses of phosphorus @ 22 kg P ha^-1as TSP, potash @ 42 kg K ha^-1 as muriate of potash, sulphur @ 20 kg S ha^-1as gypsum and zinc @ 5 kg Zn ha^-1 as zinc oxide were applied in the field. Urea was applied neither in the inoculated nor in the non-inoculated plots. Peat based rhizobial inoculum (Rhizobium strain RCa-220) @ 1.5 kg ha^-1 was used for seed inoculation. Peat based rhizobial inoculum was used containing about 10⁸ cells g^-1 inoculum.

During the course of the experiment growth and development of plants in the field were carefully observed. At 50% flowering stage, 10 randomly selected plants were uprooted from each unit plot. Dry weight of roots, shoots and nodules including nodule numbers were recorded. The plants were harvested on 20 and 25 March in 2015 and 2016, respectively. Data on plant height, 1000-seed weight (g), No. of pods per plant, No. of seeds per pod, seed yield and stover yield were also taken. All data were analyzed statistically.

Methods of chemical analysis:

_{Soil pH was measured by a combined glass calomel electrode (Jackson, 1958). Organic carbon was determined by wet oxidation method (Walkley and Black). Total N was determined by modified Kjeldahl method. Calcium, K and Mg were determined by NH₄OAc extraction method. Copper, Fe, Mn and Zn were determined by DTPA extraction followed by AAS reading. Boron was determined by CaCl₂ extraction method. Phosphorus was determined by Modified Olsen method (Neutral + Calcareous soils). Sulphur was determined by CaH₄(PO₄)₂.H₂O extraction followed by turbidimetric turbidity method with BaCl_2.}

Source of Information: Annual Research Report 2016-2017, Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh.

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

From the second year trial it may be concluded that the highest seed yield of 2.62 and 1.98 t ha^-1 and stover yield 3.60 and 4.16 t ha^-1 were recorded by inoculated BCX-05008-11 and BCX-05001-4 advance line of chickpea in 2015-2016 and 2016-2017, respectively.

Knowledge Management: Report/Proceedings