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Rescue of Amritsagar Banana from Extinction Through Biotechnological Approaches (2015-2016)

S. Islam
Horticulture Research Centre, BARI, Joydebpur, Gazipur, Bangladesh

D. Khanam
Horticulture Research Centre, BARI, Joydebpur, Gazipur, Bangladesh

M.R. Kabir
Horticulture Research Centre, BARI, Joydebpur, Gazipur, Bangladesh

M.A.Y. Akhond
Horticulture Research Centre, BARI, Joydebpur, Gazipur, Bangladesh

Abstract/ Executive Summary:

Amritsar banana collected from Goforgoan and Kapasia were compared with each other by following the standard Descriptor for Banana (Musa spp.). This comparison demonstrate that Amritsagar banana (Goforgoan) is more superior than Amritsagar banana (Kapasia) in respect of fruit length, diameter, weight and total fruit number. Shoot tips of Amritsagar banana (Goforgoan & Kapasia) were cultured on MS medium supplemented with different concentrations of BAP. Five treatments were tested for this study. Among the treatments, maximum no. of shoots was observed in T₃treatment 50.5 and 44.0 in both lines. Well-developed shoots were transferred into ½ MS medium supplemented with different conc. of IBA for root induction. Five different treatments were (T_0,T_1,T₂, T₃and T4) tested maximum numbers of longest roots were observed in both lines in treatment T_3. The plantlets were transferred into the polybag containing soil and cowdung. Banana variety Amritsagar was collected from growing at different location of Bangladesh. Among them Amritsagar banana collected from Goforgaon and Kapasia were selected for taking morphological data and for in vitro propagation preliminary according to its performance. In this experiment Descriptor for Banana (Musa spp.) recommended by the International Network for the Improvement of Banana and Plantain (INIBAP), International Plant Genetic Resources Institute (IPGRI) and Centre for International Cooperation in Agronomic Research for Development (CIRAD) was used to describe the morphological features of different Banana varieties and to make a comparison between them. In this experiment Small sucker of Amritsagar banana were carefully removed from banana plants showing no virus symptoms. The explants were prepared by removing the outer layers of tissue from suckers with a clean and sharp knife. The pale white tissue blocks (10×15 mm) containing shoot tip and rhizomatous bases were surface sterilized with Ridomil Gold MZ 68 WG for 5 min. to remove the fungal contamination. Then shoot tips were washed with Trix and detergent for 10 min. After the shoot tips were sterilized with few drops of Tween 20 in addition with 40% Sodium Hypoclorite >5.25% (clorox) aqueous solution for 15 min. followed by five times washing with sterile distilled water. The dead tissue of sterilized block was cut off to leave a 5 × 8 mm portion containing an intact apex and one or more pairs of leaf primordia together with 3-4 mm of rhizomatous bases. The prepared explants were placed on MS medium supplemented with five conc. of BAP. All cultures were incubated at 25 ± 10⁰C with a 16 h photoperiod (2000 lux) provided by cool white fluorescent tubes. The pH of the medium was adjusted to 5.8. The regenerated shoots were cultured in rooting medium containing half MS supplemented with different conc. of IBA for root induction. After root initiation the plantlets were transferred into poly bags containing soil and cowdung (1:1) and kept in net house.

Keywords: Banana, Biotechnology, Develop

Location: Horticulture Research Centre, BARI, Joydebpur, Gazipur, Bangladesh

Start Date: 00-00-2015

End Date: 00-00-2016

Program Area: Variety and Species

Commodity: Banana

Objectives:

The present research was undertaken to develop the rescue of amritsagar banana from extinction through biotechnological approaches.

Methodology:

Banana variety Amritsagar was collected from growing at different location of Bangladesh. Among them Amritsagar banana collected from Goforgaon and Kapasia were selected for taking morphological data and for in vitro propagation preliminary according to its performance. In this experiment Descriptor for Banana (Musa spp.) recommended by International Network for the Improvement of Banana and Plantain (INIBAP), International Plant Genetic Resources Institute (IPGRI) and Centre for International Cooperation in Agronomic Research for Development (CIRAD) was used to describe the morphological features of different Banana varieties and to make a comparison between them. In this experiment Small sucker of Amritsagar banana were carefully removed from banana plants showing no virus symptoms. The explants were prepared by removing the outer layers of tissue from suckers with a clean and sharp knife. The pale white tissue blocks (10×15 mm) containing shoot tip and rhizomatous bases were surface sterilized with Ridomil Gold MZ 68 WG for 5 min. to remove the fungal contamination. Then shoot tips were washed with Trix and detergent for 10 min. After then shoot tips were sterilized with few drops of Tween 20 in addition with 40% Sodium Hypoclorite >5.25% (clorox) aqueous solution for 15 min. followed by five times washing with sterile distilled water. The dead tissue of sterilized block was cut off to leave a 5 × 8 mm portion containing and intact apex and one or more pairs of leaf primordia together with 3-4 mm of rhizomatious bases. The prepared explants were placed on MS medium supplemented with five conc. of BAP. All cultures were incubated at 25 ± 10⁰C with a 16 h photoperiod (2000 lux) provided by cool white fluorescent tubes. The pH of the medium was adjusted to 5.8. The regenerated shoots were cultured in rooting medium containing half MS supplemented with different conc. of IBA for root induction. After root initiation the plantlets were transferred into poly bags containing soil and cowdung (1:1) and kept in net house.

Source of Information: Annual Research Report 2015-2016, Horticulture Research Centre, BARI, Joydebpur, Gazipur, Bangladesh

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Five treatments were tested for this study. Among the treatments, maximum no. of shoots was observed in T₃ treatment 50.5 and 44.0 in both lines. Well-developed shoots were transferred into ½ MS medium supplemented with different conc. of IBA for root induction. Five different treatments were (T₀, T₁, T₂, T₃ and T₄) tested maximum numbers of longest roots were observed in both lines in treatment T3. The plantlets were transferred into the poly bag containing soil and cowdung.

Knowledge Management: Report/Proceedings