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Pcr-based Detection and Characterization of Tomato Leaf Curl and Other Related Geminiviruses in Bangladesh (2015-2016)

M.A.Y. Akhond
Horticulture Research Centre, BARI, Joydebpur, Gazipur, Bangladesh

M. R. Kabir
Horticulture Research Centre, BARI, Joydebpur, Gazipur, Bangladesh

Abstract/ Executive Summary:

The experiment was conducted with a view to characterise Geminivirus strains in different crops beginning with tomato. Phylogenetic analysis was carried out with sequence data of the complete ’A’ genome of 32 isolates that have been submitted to NCBI database and compared with related viruses from different parts of the world. Virus infected cucurbit leaf samples from five districts of Bangladesh were collected and DNA from the infected leaf samples along with the virus particles was isolated. Virus infected leaf samples of several other plants including okra, yardlong bean, bean, soybean, mungbean and blackgram have also been collected and DNA extracted for further analysis.

Keywords: Characterize, Geminivirus, Crops, Tomato.

Location: Horticulture Research Centre, BARI, Joydebpur, Gazipur, Bangladesh

Start Date: 00-00-2015

End Date: 00-00-2016

Program Area: Pest Management

Commodity: Virus, Tomato

Objectives:

To characterize geminivirus strains in different crops with emphasis to tomato.

Methodology:

Virus-specific primers were designed on the basis of the initial sequences and the whole ‘A’ genome of 32 isolates were sequenced. Sequence fragments for each sample were assembled in computer using ‘Geneious’ software. Identities of the isolates were confirmed by using pairwise identitiy analysis. All the sequences were aligned using the ‘Muscle’ software and a phylogenetic tree was constructed for comparison. Sequence alignment and the tree construction (using Maximum likelihood method with 1000 replications) were carried out from within the sequence analysis software (MEGA). DNA of 60 cucurbit samples collected from 5 districts of the country was extracted. Infected leaf samples from okra, soybean, mungbean and blackgram has also been collected and DNA was extracted for further analysis.

Source of Information: Annual Research Report 2015-2016, Horticulture Research Centre, BARI, Joydebpur, Gazipur, Bangladesh

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Virus infected cucurbit leaf samples from five districts of Bangladesh were collected and DNA from the infected leaf samples along with the virus particles was isolated. Virus infected leaf samples of several other plants including okra, yardlong bean, bean, soybean, mungbean and blackgram have also been collected and DNA extracted for further analysis.

Knowledge Management: Report/Proceedings