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Production of Breeder’s Propagule of Transgenic Late Blight Resistant Potato Clones Through Tissue Culture (2015-2016)

M.M. Khatun
Horticulture Research Centre, BARI, Joydebpur, Gazipur, Bangladesh

S. C. Halder
Horticulture Research Centre, BARI, Joydebpur, Gazipur, Bangladesh

D. Khanam
Horticulture Research Centre, BARI, Joydebpur, Gazipur, Bangladesh

Abstract/ Executive Summary:

Transgenic SP 951(137) was used as parent materials. Sprouts of this clone were used as explant. Sprouts were cultured on PROP medium and multiplied in vitro. Initially, 30 tubers were used as a source of explants after in vitro multiplication these materials went through an ELISA test to detect the virus-free explant source and we had found 10 explants plant sources free from virus. Subsequent subculture was done only of the virus-free materials to produce the virus-free plantlet. This virus-free plantlet will be maintained in vitro as well as it will be grown under greenhouse conditions to produce mini tubers as breeder’s propagule.

Keywords: Breeder’s propagule, Transgenic, Potato clones, Tissue culture

Location: Horticulture Research Centre, BARI, Joydebpur, Gazipur, Bangladesh

Start Date: 00-00-2015

End Date: 00-00-2016

Program Area: Pest Management

Commodity: Virus, Potato

Objectives:

To the production of breeder’s propagule of transgenic late blight resistant potato clones through tissue culture.

Methodology:

Sprouts of transgenic SP 951(137) was used as an explant source, For inoculation of explants firstly potato sprouts were surface-sterilized under running tap water for 30 min. with jet and trix to remove the surface contamination outside the clean bench. After that the sprouts were taken inside the laminar airflow cabinet in an autoclaved jar and dipped in 99% ethanol plus 2 drops of Tween - 20 for 20 sec. and washed with autoclaved distilled water 2 to 3 times. Finally, sprouts were sterilized with clorox solution (60 ml Clorox: 40 ml distilled water) for 1 min. and then washed for 3 to 4 times with autoclaved distilled water. After that the sterilized sprouts were excised with the help of the sterilized scalpel and forceps. Then the prepared explants were placed in text tubes/baby jars containing previously prepared media. The inoculated cultures were placed in growth room at 25 ± 1⁰C under approx. 2000 lux by cool white fluorescent tubes for 16/8 light/dark cycle.

Source of Information: Annual Research Report 2015-2016, Horticulture Research Centre, BARI, Joydebpur, Gazipur, Bangladesh

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The last year trialed six transgenic clones only one transgenic clone SP 951(137) especially is now maintained in our lab and greenhouse to produce virus-free breeder propagule but it is very limited scale.

Knowledge Management: Report/Proceedings