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Transformation of Tomato for Broad-spectrum Resistance Against Leaf Curl Viruses (2015-2016)

M.A.Y. Akhond
Horticulture Research Centre, BARI, Joydebpur, Gazipur, Bangladesh

M. R. Kabir
Horticulture Research Centre, BARI, Joydebpur, Gazipur, Bangladesh

Abstract/ Executive Summary:

Several experiments were conducted with a view to genetically transform tomato plants for broad-spectrum resistance against leaf curl viruses. Based on the genome sequence of various ToLCV strains, DNA fragments from three diverse ToLCV species were amplified and cloned. Two plasmid vectors were constructed for transformation work. Shoot regeneration has been observed from tomato explants following co-cultivation with Agrobacterium harbouring one of the binary vectors. Infectious clones of the most virulent leaf curl virus species were constructed and Nicotiana benthamiana indicator plants were agroinoculated resulting in the expression of leaf curl disease symptoms.

Keywords: Leaf curl viruses, Tomato, Agrobacterium

Location: Horticulture Research Centre, BARI, Joydebpur, Gazipur, Bangladesh

Start Date: 00-00-2015

End Date: 00-00-2016

Program Area: Pest Management

Commodity: Virus

Objectives:

To genetically transform tomato plants for broad-spectrum resistance against leaf curl viruses.

Methodology:

Specific primers were designed and specific sequence fragments from the three most virulent species were amplified by PCR and cloned into Escherichia coli. Two intron sequences were amplified and cloned from tomato following the same protocol. Various DNA fragments were assembled for making two binary vectors and confirmed by sequencing. One confirmed vector was mobilized into Agrobacterium and subsequently used for agroinoculation and genetic transformation. Infectious clones of the most virulent leaf curl virus species were constructed and Nicotiana benthamiana indicator plants were agroinoculated.

Source of Information: Annual Research Report 2015-2016, Horticulture Research Centre, BARI, Joydebpur, Gazipur, Bangladesh

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Shoot regeneration has been observed from tomato explants following co-cultivation with Agrobacterium harbouring one of the binary vectors. Infectious clones of the most virulent leaf curl virus species were constructed and Nicotiana benthamiana indicator plants were agroinoculated resulting in the expression of leaf curl disease symptoms.

Knowledge Management: Report/Proceedings