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In Vitro Study of Antiamoebic Effect of Methanol Extract of Mature Seeds of Carica Papaya on Trophozoites of Entamoeba Histolytica

Sujit Kumar Sarker
Department of Pharmacology and Therapeutics, Dhaka Medical College, Dhaka 1000, Bangladesh

Nilufar Begum
Department of Pharmacology and Therapeutics, Shahabuddin Medical College, Dhaka 1212, Bangladesh

Dinesh Mondal
Parasitology Laboratory, ICDDR,B Dhaka 1212, Bangladesh

Md. Abdullah Siddique
Parasitology Laboratory, ICDDR,B Dhaka 1212, Bangladesh

Mohammad A. Rashid
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka 1000, Bangladesh

Abstract/ Executive Summary:

Antiamoebic activity of methanol extract of mature seeds of Carica papaya was tested in vitro on axenic culture of Entamoeba histolytica using metronidazole as a reference amoebicidal agent. The MIC of seed extract was >62.5 pg/mL as compared to <0.8 pg/mL for metronidazole. The present study suggests that the mature seeds of C. papaya have antiamoebic effect but less pronounced than metronidazole.

Keywords: Amoebiasis, Entamoeba histolytica, Carica papaya

Location: Manikgonj, Bangladesh

Start Date: 00-11-2006

End Date:

Program Area: Resource Development and Management

Commodity: Papaya

Objectives:

The present study was conducted to evaluate the effectiveness of mature seeds of papaya against E. histolytica, using metronidazole as a reference standard.

Methodology:

Plant materials

Dried mature seeds of C. papaya were collected from Manikgonj in November, 2006. The plants were identified at Bangladesh National Herbarium, Dhaka where a voucher specimen has been deposited (DACB accession number was 31374) for this collection.

Preparation of extract

Dried papaya seeds were first ground to a coarse powder and then methanol extract was prepared by macerating powdered seeds in methanol for a week. Then the slurry was first filtered and solvent was evaporated under reduced pressure by using a rotary evaporator. Then the extract thus obtained was stored until in vitro antiamoebic test.

Culture of E. histolytica

DS 4 868 strain of E. histolytica was used in the present study. Here, established axenic culture of E. histolytica and LYI-S-2 culture media was kindly provided by Dr. Rashidul Haque, Head of Parasitology Laboratory, ICDDR,B Dhaka.

Serial dilutions

Serial dilutions of stock solution in culture medium were conducted in 24 well culture plates. For the extract, serial dilutions were conducted to get 2000 to 15.6 pg/mL and for metronidazole solutions were made from 200 to 0.8 pg/mL.

Trials of the study

Several trials were conducted for the study. Each trial included blank (only culture medium), control (culture medium and E. histolytica) and test (extract or metronidazole, culture medium and E. histolytica).

Count of E. histolytica

After incubation for 48 hours at 37°C viable trophozoites of E. histolytica were counted with the aid of Neubauer's cell of Hemocytometer.

Source of Information: Bangladesh Journal of Pharmacology (Bangladesh) Vol 5 No 1 (2010) /

Article Link (Online Journal): DOI: 10.3329/bjp.v5i1.5003

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The present study suggests that metronidazole is more potent than the methanol extract of C. papaya.

Knowledge Management: Journal