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Genetic Diversity Based on Coat Protein of Papaya Ringspot Virus (pathotype P) Isolates from Bangladesh

M. S. Akhter
Fruit Research Station, Bangladesh Agricultural Research Institute, Rajshahi, 6206 Bangladesh

Y. B. Basavaraj
Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012 India

A. M. Akanda
Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 1706 Bangladesh

B. Mandal
Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012 India

R. K. Jain
Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012 India

Abstract/ Executive Summary:

The coat protein (CP) sequences of twelve Papaya ringspot virus (PRSV) (pathotype-P) isolates from six major papaya growing areas were determined and compared with those of published PRSV. The CP coding region varied in size from 846–852 nucleotides, encoding a protein of 282–284 amino acids. Comparative CP sequence analysis revealed that the PRSV-P isolates originating from Bangladesh were divergent up to 14 % at amino acids level. Further, the isolates from Bangladesh shared 86–95 % amino acid sequence identity with those reported from rest 21 of the Asia and 83–93 % amino acid sequence identity with isolates from the other parts of the world. A number of KE repeats were observed in the N terminus of the CP coding region of all Bangladesh isolates. Phylogenetic branching pattern revealed that the PRSV-P isolates originating from Bangladesh formed a distinct clade from those from the rest of the world. This forms the first report on the genetic diversity of PRSV-P isolates from Bangladesh.

Keywords: Papaya ringspot virus, Pathotype-P, Bangladesh isolates, Coat protein

Location: December 2010 to February 2011 in four different districts in the northern and central parts of Bangladesh—Rajshahi, Chapai Nowabgong, Pabna and Gazipur

Start Date: 00-12-2010

End Date: 00-02-2011

Program Area: Variety and Species

Commodity: Papaya, Virus

Objectives:

The present study was thus undertaken to characterize the genetic diversity of PRSV-P isolates occurring in the major papaya producing regions in Bangladesh.

Methodology:

A field survey was conducted during December 2010 to February 2011 in four different districts in the northern and central parts of Bangladesh—Rajshahi, Chapai Nowabgong, Pabna and Gazipur. In Gazipur district, survey was conducted on three locations, Bangladesh Agricultural Development Corporation (BADC) farm, Kashimpur; Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU) farm, Salna; and farmer papaya orchards at Mirzapur. Percent disease incidence and reduction in plant height and number of fruits/plant in comparison to the healthy plants were calculated using standard statistical tools. Suspected PRSV affected papaya samples (95) from six different locations were collected and lyophilized for further study. Presence of the virus in lyophilized samples was confirmed by direct antigen-coated enzyme-linked immuno-sorbent assay (DAC-ELISA) using polyclonal antibodies (PAb) to PRSV developed at IARI, New Delhi as described by Clark and Bar-Joseph [3]. Leaf tissue (500 mg) was ground in 1 ml extraction buffer and 200 μl samples were used in duplicates for ELISA. The PAb to PRSV was used at 1:2000 dilutions. The reaction was recorded 1 h after adding substrate by an ELISA reader (BIO-TEK Instruments, USA). ELISA positive samples (12, two from each location) were subjected to reverse transcription and polymerase chain reaction (RT-PCR) [4].

Source of Information: Indian J Virol. 2013 Jun; 24(1): 70–73.

Article Link (Online Journal): doi: 10.1007/s13337-013-0127-2

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The present study provides a partial profile of PRSV-P population in Bangladesh. More sequence data need to be generated. The existing sequence diversity will have significant impact on devising PRSV management strategy in Bangladesh through CP gene derived transgenic resistance.

Knowledge Management: Journal