Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Microbiological Quality Assessment for Drug Resistant Pathogenic Microorganisms from the Fresh Vended Fruit Juices

Nurunnahar Akter Lucky
Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

Ifra Tun Nur
Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

Tasnia Ahmed*
Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

Abstract/ Executive Summary:

Fruit juices are very popular due to their freshness and taste. These juices contain all the nutritive values those were present in whole fruits. Fresh juices are healthful drink for people of all ages. But the condition is quite opposite if they become contaminated with pathogenic bacteria during the juice processing. Because of the nutritive properties juice can give enough opportunities to the microorganisms to survive and multiply. In our current study we included eight types of fresh juices to study the microbial load. All the samples were heavily contaminated. Total bacterial and fungal count was found to be up to 3.0×107 cfu/ml and 2.6×106 cfu/ml, respectively. Salmonella spp. and Shigella spp. was present only in one sample. Staphylococcus aureus and Pseudomonas spp. were the most prominent in all of the eight samples. Vibrio spp. was found in six samples ranging from 2.0×102 cfu/ml to 2.9×104 cfu/ml. All the pathogenic isolates showed drug resistance towards the most commonly prescribed antibiotics indicating the risk of difficulty in eradicating diseases.

Keywords: Fruit juices; Contamination; Drug resistance

Location: Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

Start Date: 00-02-2015

End Date: 00-04-2015

Program Area: Quality and Nutrition

Commodity: Juice

Objectives:

The current study was designed to determine the presence of pathogenic bacteria found in fresh vended fruit juices and their drug resistance traits sold in different areas of Dhaka city to identify the risk of public health.

Methodology:

Study area and sample processing. Fruit juices sold in different areas were subjected to microbiological analysis. The study included eight different juices such as sour mango, apple, wood apple, papaya, mixed fruit, banana-milk, sugarcane and orange juice. Fresh fruit juices from vendors were collected during the time span of February 2015 to April 2015. Samples were collected aseptically and transferred to the microbiological laboratory for microbial assay. The juices were homogenized in normal saline and serially diluted up to 10-5 (24). Microbiological assay. 0.1 ml of homogenized fruit juices were inoculated onto nutrient agar (NA), Sabouraud dextrose agar (SDA), Pseudomonas agar, MacConkey agar, mannitol salt agar to detect total viable bacterial count, total fungal count, Pseudomonas spp., Escherichia coli, Klebsiella spp., Staphylococcus aureus respectively. For detection of VBNC (viable but non culturable) bacteria, 1ml of fruit juice was introduced into 9ml of alkaline peptone water (APW) and selenite cysteine broth (SCB) for enrichment and after that inoculated onto Thiosulfate Citrate Bile Salt Sucrose (TCBS) agar and Salmonella-Shigella (SS) agar for the detection of Vibrio spp., Salmonella spp., and Shigella spp. (25, 26). SDA plates were incubated at 25 ^oC for 48 hours and all the other plates were incubated at 37 ^oC for 24 hours. Study for antibiotic susceptibility. To demonstrate the drug resistance pattern of the pathogenic isolates found in the fresh fruit juices were subjected to observe their drug resistance traits against most commonly used antibiotics. Suspensions were prepared using the isolates and after getting the desired turbidity inoculated onto Muller-Hinton agar (MHA) plates using a sterile cotton swab. After that some pre-selected antibiotic discs were placed over the media and incubated at 37 ^oC for 24 hours. After the incubation period plates were observed to determine the zone of inhibition indicating the susceptibility against the antibiotic. Antibiotics which were used for this segment of our study include Streptomycin (10 µg), Vancomycin (30µg), Ciprofloxacin (5µg), Ceftriaxone (30µg), Gentamicin (10µg), Kanamycin (30µg), Amoxicillin (10µg), Nalidixic acid (30µg), Ampicillin (10µg), Erythromycin (15µg) and Chloramphenicol (10µg). Normal saline was used as negative control.

Source of Information: Stamford Journal of Microbiology, 2016. Vol. 6, Issue 1, 7-10 ISSN: 2074-5346 (Print); 2408-8846 (Online)

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Fruit juices are very popular drink with adequate amount of vitamins and minerals which is very important for our health. But this nutritious substance can also be harmful for the health if harbors adequate amount of pathogenic bacteria. Pathogenic bacteria can be introduces from the raw fruits, ingredients, equipments and multiply rapidly using the nutrition of a the fruit juice. Food law enforcement should be strict enough to control the condition specially in case of street vending.

Knowledge Management: Journal